灵芝三萜液态深层发酵的研究进展

doi:10.11923/j.issn.2095-4050.cjas2018-1-126

农学学报 ›› 2018, Vol. 8 ›› Issue (1): 126-132.doi: 10.11923/j.issn.2095-4050.cjas2018-1-126

所属专题：生物技术；农业学科与产业发展

灵芝三萜液态深层发酵的研究进展

翟双星¹^,², 冯杰¹(), 冯娜¹, 唐庆九¹, 周帅¹, 杨焱¹, 刘艳芳¹, 张劲松¹()

¹ 上海市农业科学院食用菌研究所/国家食用菌工程技术研究中心/农业部南方食用菌资源利用重点实验室/国家食用菌加工分中心/上海市农业遗传育种重点开放实验室,上海 201403
² 上海师范大学生命与环境科学学院,上海 200234

收稿日期:2017-11-27 修回日期:2017-12-28 出版日期:2018-01-20 发布日期:2020-04-22
通讯作者: 冯杰,张劲松 E-mail:sytufengjie@163.com;18918162047@189.cn
作者简介:翟双星,女,1993年生,安徽宿州人,硕士研究生,研究方向：生物化学与分子生物学生物药物学。通信地址：201403 上海市奉贤区南桥镇金齐路1000号上海市农业科学院食用菌研究所413,Tel：021-37196810,E-mail：18016264266@163.com。
基金资助:
灵芝抑制前列腺增生功能因子关键制备技术的研究(沪农科攻字2016第5-4号)

Research Progress on Ganoderma triterpenes Obtained by Liquid Submerged Fermentation

Zhai Shuangxing¹^,², Feng Jie¹(), Feng Na¹, Tang Qingjiu¹, Zhou Shuai¹, Yang Yan¹, Liu Yanfang¹, Zhang Jingsong¹()

¹ Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, P. R. China, National R&D Center for Edible Fungi Processing; Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Shanghai 201403, China;
² College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China

Received:2017-11-27 Revised:2017-12-28 Online:2018-01-20 Published:2020-04-22
Contact: Feng Jie,Zhang Jingsong E-mail:sytufengjie@163.com;18918162047@189.cn

摘要/Abstract

摘要：

三萜类化合物是灵芝中主要的活性化学成分之一,由于其具有多种生理活性,现已成为灵芝研究开发的热点。为了给灵芝三萜液态深层发酵的调控及未来的规模化生产提供参考和借鉴,本研究聚焦通过发酵工程手段获得灵芝三萜的最新研究工作,综述了灵芝发酵方法中三萜的检测方法、发酵技术、液态深层发酵动力学及与灵芝三萜生物合成相关途径等方面的研究进展。在此基础上指出了研究中存在的问题：没有系统性地筛选适合发酵工程生产的灵芝三萜菌株,缺乏对菌丝体中三萜的化学结构和药理活性方面的研究,目前只有较少规模化的利用发酵法生产灵芝三萜的研究,缺少直接利用活性追踪的方法优化灵芝三萜生产工艺等,最后进行了展望。

关键词: 灵芝, 发酵, 发酵动力学, 生物合成

Abstract:

Triterpenes are one of the main active chemical components in Ganoderma, and it has become the hotspot in current research due to multiple physiological activities. In order to provide a reference for the liquid submerged fermentation regulation of Ganoderma triterpenes and large-scale production, we focus on the recent latest research of Ganoderma triterpenes produced by fermentation engineering. In addition, the progress of the detection method of triterpenes, fermentation technology, fermentation kinetics, and the triterpenes biosynthesis pathway was reviewed. On this basis, the problems in research were as follows: we had not screened Ganoderma triterpenes strains systematically suitable for the fermentation production, we lacked the study of triterpenes chemical constitution and pharmacological activity, there was only less study on the Ganoderma triterpenes large-scale production by fermentation, and we lacked the production engineering of Ganoderma triterpenes optimized by activity tracking. At last, the progress was forecasted.

Key words: Ganoderma, Fermentation, Fermentation Kinetics, Biosynthesis

中图分类号:

翟双星, 冯杰, 冯娜, 唐庆九, 周帅, 杨焱, 刘艳芳, 张劲松. 灵芝三萜液态深层发酵的研究进展[J]. 农学学报, 2018, 8(1): 126-132.

Zhai Shuangxing, Feng Jie, Feng Na, Tang Qingjiu, Zhou Shuai, Yang Yan, Liu Yanfang, Zhang Jingsong. Research Progress on Ganoderma triterpenes Obtained by Liquid Submerged Fermentation[J]. Journal of Agriculture, 2018, 8(1): 126-132.

图/表 2

图1. 灵芝三萜的基本骨架结构

图2. 灵芝酸的生物合成途径 MVA：甲羟戊酸;MVK：甲羟戊酸激酶;MVAP：甲羟戊酸磷酸;MPK：甲羟戊酸磷酸激酶;MVPP：甲羟戊酸二磷酸;MVD：焦磷酸甲羟戊酸脱羧酶;IPP：异戊烯基焦磷酸酯;IDI：异戊烯基焦磷酸异构酶;FPP：法呢酰二磷酸酯;FPPS：法呢基二磷酸合成酶;SQS：鲨烯合成酶;GGPP：牻牛儿基焦磷酸;PPP：多聚异戊二烯二磷酸;LSS：羊毛甾醇合成酶

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灵芝三萜液态深层发酵的研究进展

Research Progress on Ganoderma triterpenes Obtained by Liquid Submerged Fermentation

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