国内葡萄白粉病菌对戊唑醇的抗药性研究

doi:10.11923/j.issn.2095-4050.cjas2022-0063

农学学报 ›› 2023, Vol. 13 ›› Issue (6): 49-54.doi: 10.11923/j.issn.2095-4050.cjas2022-0063

国内葡萄白粉病菌对戊唑醇的抗药性研究

高琪¹^,²(), 刘梅¹, 谭海芸¹^,², 王琦², 李兴红¹, 张玮¹()

¹ 北方果树病虫害绿色防控北京市重点实验室/北京市农林科学院植物保护研究所，北京 100097
² 中国农业大学植物保护学院，北京 100193

收稿日期:2022-05-24 修回日期:2022-09-29 出版日期:2023-06-20 发布日期:2023-06-15
通讯作者: 张玮，女，1986年出生，山东德州人，副研究员，研究方向：果蔬病害诊断与防控技术。通信地址：100097 北京市海淀区曙光花园中路9号北京市农林科学院植物保护研究所，E-mail：zhwei1125@163.com。
作者简介:
高琪，女，1996年出生，河北邢台人，硕士研究生。通信地址：100097 北京市海淀区曙光花园中路9号北京市农林科学院植物保护研究所，E-mail：gaoqi977@163.com。
基金资助:
财政部和农业农村部国家现代农业产业技术体系“树体病害防控岗位”(CARS-29); 北京市科技计划课题“设施有机生产绿色生防技术研究与示范”(Z201100008020014)

Resistance of Erysiphe necator to Tebuconazole in China

GAO Qi¹^,²(), LIU Mei¹, TAN Haiyun¹^,², WANG Qi², LI Xinghong¹, ZHANG Wei¹()

¹ Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China/ Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
² College of Plant Protection, China Agricultural University, Beijing 100193, China

Received:2022-05-24 Revised:2022-09-29 Online:2023-06-20 Published:2023-06-15

摘要/Abstract

摘要：

为明确国内葡萄白粉病菌(Erysiphe necator)对戊唑醇的抗药性，采用实时荧光定量PCR，基于对葡萄白粉病菌CYP51基因第495位点突变A495T的检测，分析国内5个省份分离获得的134株菌株对戊唑醇的抗药性，利用孢子萌发法测定供试菌株对戊唑醇的敏感性，分析2种方法结果的相关性。结果表明，实时荧光定量PCR方法检测到国内葡萄白粉病菌对戊唑醇已出现抗药性，抗性频率为35.07%，不同地区存在差异。孢子萌发法结果显示，供试菌株对戊唑醇的EC₅₀范围为0.085~280.917 μg/mL，均值为24.208 μg/mL，不同地区菌株对戊唑醇的敏感性差异较大，EC₅₀最大值和最小值之比为2.5~3304。孢子萌发法与实时荧光定量PCR方法间的检测结果趋势一致并显著相关。

关键词: 葡萄白粉病菌, 戊唑醇, 抗药性, 实时荧光定量PCR, 孢子萌发

Abstract:

To clarify the sensitivity of Erysiphe necator isolates to tebuconazole in China, quantitative real-time PCR (qPCR) was used to analyze the resistance of 134 E. necator isolates collected from 5 grape growing provinces, based on the detection of mutation A495T at locus 495 of the CYP51 gene of E. necator. The spore germination method was used to determine the susceptibility of the test isolates to tebuconazole, and the correlation between the results of the two methods was analyzed. The results showed that E. necator isolates had developed resistance to tebuconazole in China, while the resistance frequency was 35.07%, with differences in different regions. The EC₅₀ value of all isolates to tebuconazole determined by spore germination method ranged from 0.085 to 280.917 μg/mL and the average EC₅₀ value was 24.208 μg/mL. The ratio of the lowest and the highest EC₅₀ values for the isolates from different regions ranged from 2.5 to 3304, indicating that the sensitivity of the isolates from different regions to tebuconazole varied greatly. The results of tebuconazole sensitivity detection of the spore germination method and qPCR method were consistent and significantly correlated.

Key words: Erysiphe necator, tebuconazole, fungicide resistance, quantitative real-time PCR, spore germination

高琪, 刘梅, 谭海芸, 王琦, 李兴红, 张玮. 国内葡萄白粉病菌对戊唑醇的抗药性研究[J]. 农学学报, 2023, 13(6): 49-54.

GAO Qi, LIU Mei, TAN Haiyun, WANG Qi, LI Xinghong, ZHANG Wei. Resistance of Erysiphe necator to Tebuconazole in China[J]. Journal of Agriculture, 2023, 13(6): 49-54.

图/表 4

参考文献 32

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采集地点	菌株数/株	抗性菌株数/株	A495T频率/%
北京市密云区	4	4	100
北京市朝阳区	14	5	35.71
北京市平谷区	22	6	27.27
北京市通州区	26	5	19.23
北京市房山区	21	2	9.52
湖南省岳阳市	6	3	50
江苏省张家港市	15	6	40
江苏省昆山市	20	3	15
云南省元谋县	3	1	33.33
宁夏自治区银川市	3	2	66.66
总计	134	47	35.07

采集地点	菌株数/株	抗性菌株数/株	A495T频率/%
北京市密云区	4	4	100
北京市朝阳区	14	5	35.71
北京市平谷区	22	6	27.27
北京市通州区	26	5	19.23
北京市房山区	21	2	9.52
湖南省岳阳市	6	3	50
江苏省张家港市	15	6	40
江苏省昆山市	20	3	15
云南省元谋县	3	1	33.33
宁夏自治区银川市	3	2	66.66
总计	134	47	35.07

采集地点	菌株数/株	EC₅₀/(μg/mL)			敏感性指数
采集地点	菌株数/株	范围	差异倍数/倍	平均数±标准差	敏感性指数
北京市密云区	4	18.377~114.826	6.2	51.164±43.271	2.11
北京市朝阳区	14	3.343~49.603	14.8	21.396±13.361	0.88
北京市平谷区	22	4.358~43.424	9.9	19.247±11.937	0.79
北京市通州区	26	2.969~41.769	14.1	18.139±9.074	0.67
北京市房山区	21	2.580~59.048	22.9	16.549±13.573	0.75
湖南省岳阳市	6	4.851~28.642	5.9	17.471±9.383	0.72
江苏省张家港市	15	7.048~52.695	7.5	23.226±14.797	0.95
江苏省昆山市	20	4.559~70.519	15.5	31.123±14.797	1.29
云南省元谋县	3	7.438~18.314	2.5	13.996±5.774	0.58
宁夏自治区银川市	4	0.085~280.917	3304	195.198±180.968	8.06
总计	134	0.085~280.917	3304	24.208±28.039	1

采集地点	菌株数/株	EC₅₀/(μg/mL)			敏感性指数
采集地点	菌株数/株	范围	差异倍数/倍	平均数±标准差	敏感性指数
北京市密云区	4	18.377~114.826	6.2	51.164±43.271	2.11
北京市朝阳区	14	3.343~49.603	14.8	21.396±13.361	0.88
北京市平谷区	22	4.358~43.424	9.9	19.247±11.937	0.79
北京市通州区	26	2.969~41.769	14.1	18.139±9.074	0.67
北京市房山区	21	2.580~59.048	22.9	16.549±13.573	0.75
湖南省岳阳市	6	4.851~28.642	5.9	17.471±9.383	0.72
江苏省张家港市	15	7.048~52.695	7.5	23.226±14.797	0.95
江苏省昆山市	20	4.559~70.519	15.5	31.123±14.797	1.29
云南省元谋县	3	7.438~18.314	2.5	13.996±5.774	0.58
宁夏自治区银川市	4	0.085~280.917	3304	195.198±180.968	8.06
总计	134	0.085~280.917	3304	24.208±28.039	1

国内葡萄白粉病菌对戊唑醇的抗药性研究

Resistance of Erysiphe necator to Tebuconazole in China

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