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农学学报

农学学报 ›› 2014, Vol. 4 ›› Issue (12): 30-32.doi: 10.11923/j.issn.2095-4050.2014-xb0213

• 植物保护 • 上一篇    下一篇

山东灰霉病菌对腐霉利的抗药性检测

刘超,张悦丽,张博,李长松,石磊,齐军山   

  1. 曲阜师范大学生命科学学院,山东省农业科学院植物保护研究所,山东省农业科学院植物保护研究所,山东省农业科学院植保所,曲阜师范大学,山东省农业科学院植物保护研究所
  • 收稿日期:2014-03-11 修回日期:2014-11-30 接受日期:2014-04-29 出版日期:2014-12-29 发布日期:2014-12-29
  • 通讯作者: 齐军山 E-mail:liuchao8804
  • 基金资助:
    公益性行业（农业）科研专项经费“保护地果蔬灰霉病绿色防控技术研究与示范”(201303025)；山东省现代农业产业技术体系；山东省农业科学院科技创新重点项目（2014CXZ07）。

Resistance Detection for Botrytis cinerea Against Procymidone in Shandong Province

齐军山   

  • Received:2014-03-11 Revised:2014-11-30 Accepted:2014-04-29 Online:2014-12-29 Published:2014-12-29

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摘要： 为监测灰霉病菌对腐霉利的抗药性，2012 年12 月到2013 年5 月，在山东省内10 个市县，采集灰霉病菌样本150 余份，分离得到灰葡萄孢(Botrytis cinerea)菌株130 株，采用生长速率法对130 株灰霉病菌进行抗药性检测。结果表明，腐霉利对灰霉病菌的EC50的范围为0.1208~12.486 μg/mL，平均EC50为4.03 μg/mL，抗性频率达到67.69%。其中低抗菌株占52.38%，中抗菌株占19.048%，未检测到高抗菌株。本研究可为灰霉病的防治特别是杀菌剂的轮换使用提供依据。

关键词: 种衣剂, 种衣剂, 紫苏, 发芽率, 产量, 品质, 抗性

Abstract: In order to monitor procymidone resistance ofBotrytis cinerea , from December of 2012 to May of 2013, manyBotrytis cinerea samples were collected from 10 cities in Shandong, and 130 isolates were purified from them. The resistances of Botrytis cinerea were determined in 130 isolates by measuring the mycelia growth on the fungicide-amended media. The means of the 50% effective concentration values (EC 50) were 4.03 μg/mL. The resistance frequency was 67.69% , 52.38% of Botrytis cinerea was low resistant strains, 19.048% ofBotrytis cinerea was moderate resistant strains; there was no high resistant strain. The resultsprovided a theoretical basis for using fungicides efficiently to prevent and controlbotrytis cinerea in the field.