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农学学报

农学学报 ›› 2012, Vol. 2 ›› Issue (6): 27-30.

所属专题： 生物技术； 小麦

• 农艺科学 作物遗传育种 生理生化 • 上一篇    下一篇

黑粒小麦‘冬黑10号’的细胞学和麦谷蛋白组成分析

郭明慧 裴自友 温辉芹 张立生 程天灵 李雪   

  • 收稿日期:2012-03-06 修回日期:2012-03-25 出版日期:2012-06-20 发布日期:2012-06-20
  • 基金资助:

    山西省回国留学人员科研资助项目;山西省科技攻关项目;山西省农业科学院科研项目

Analysis of Cytology and Glutenin Composition in Black Kernel Wheat ‘Donghei 10’

  • Received:2012-03-06 Revised:2012-03-25 Online:2012-06-20 Published:2012-06-20

PDF (PC)

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摘要：

山西省作为中国优质小麦重要产区，目前已育成6个黑粒小麦品种，然而对这些黑粒小麦的染色体构成、蛋白组分却研究较少。因此，了解其遗传组成对选育黑粒小麦新品种具有指导作用。利用染色体C-分带，减数分裂中期Ⅰ染色体配对构型、基因组原位杂交结合谷蛋白亚基分析，对黑粒小麦‘冬黑10号’的染色体构成进行系统鉴定。结果表明，‘冬黑10号’体细胞染色体数目为2n=42，染色体带型与‘中国春’染色体带型未见明显变化，基因组原位杂交也未检出赖草染色体；其PMC MⅠ染色体平均构型为21Ⅱ，具有良好的细胞学稳定性，与‘中国春’小麦杂交F1的PMC MⅠ染色体构型为21Ⅱ，说明其为六倍体普通小麦。SDS-PAGE分析揭示其高分子量麦谷蛋白亚基组成为Null/7+9/5+10，低分子量麦谷蛋白亚基与对照‘中国春’不同。

关键词: 生物炭, 生物炭, 木醋液, 减量农药, 烟草, 病害

Abstract:

Shanxi Province is a important producing area of quality wheat in China, had been developed and approved six black kernel wheat varieties by Shanxi Crop Variety Approval Committee, however, their chromosome and protein constitutions of these cultivars are less reported. Understanding its genetic composition will provide useful information for breeding. In this paper, chromosome configuration of black kernel wheat Donghei 10 was characterized by chromosome C-banding, genomic in situ hybridization, chromosome pairing configuration and glutenin subunit analysis. The results showed that the chromosomes numbered 42(2n= 42) in its root tip-cells, chromosome C-banding patterns no significant changes from Chinese Spring, in the genomic in situ hybridization were not detected Leymus secalinus chromosomes. The mean chromosome pairing configuration was 2n= 21 II at PMC MⅠ, indicating Donghei 10 with good stability of cytology, and F1 hybrids with Chinese spring PMC MⅠ chromosome configuration of 21 II showed to be hexaploid common wheat. SDS-PAGE analysis revealed that HMW-GS composed of Null/7+9/5+10, in addition to LMW-GS differing from that of Chinese Spring.