欢迎访问《农学学报》，
农学学报

农学学报 ›› 2012, Vol. 2 ›› Issue (12): 37-41.

• 林学 园艺 园林 食用菌 • 上一篇    下一篇

影响蕙兰‘大一品’根状茎芽分化的因素研究

于永畅   

  • 收稿日期:2012-08-24 修回日期:2012-10-12 出版日期:2012-12-20 发布日期:2012-12-20
  • 基金资助:

    国兰新品种培育与产业化生产关键技术研究

Study on the Influencing Factors of Shoot Differentiation of Rhizome in Cymbidium faberi ‘Dayipin’

  • Received:2012-08-24 Revised:2012-10-12 Online:2012-12-20 Published:2012-12-20

PDF (PC)

1289

摘要：

为了解决蕙兰组织培养过程中分化速度慢的问题，以蕙兰名品‘大一品’自交种的根状茎为材料，系统研究基本培养基、植物生长调节剂、有机附加物、P/N比、碳源、蔗糖浓度6个因素对根状茎芽分化的影响。结果表明：MS培养基为蕙兰‘大一品’根状茎芽分化的最佳培养基；植物生长调节剂浓度配比对芽分化率影响显著，在含NAA 0.3 mg/L+ 6-BA 2.0 mg/L的培养基上芽分化率最高；有机附加物对根状茎芽诱导和分化有促进作用，添加100 g/L香蕉泥能显著提高根状茎芽诱导率和分化率；减少培养基中N的含量(1/10N)，增加P含量(5P)，可以提高芽诱导率和分化率；麦芽糖为最适合芽诱导和分化的碳源；20 g/L蔗糖对芽分化有显著的促进作用。

关键词: 人工栽培甘草, 人工栽培甘草, 机械中耕, 生长

Abstract:

In order to study the rapid differentiation of rhizomes of Cymbidium faberi in tissue culture. The effects of basic medium, plant growth regulator, organic additives, P/N, carbon sources and sucrose concentration on the shoot differentiation of rhizomes were studied through the rhizomes from the self-cross seeds of Cymbidium faberi ‘Dayipin’. The results showed that the most appropriate cultivating medium was MS. The plant growth regulator had a significant effect on shoot differentiation. The highest shoot differentiation rate was appeared on 0.3 mg/L NAA+ 2.0 mg/L 6-BA. The organic additives promoted the shoot induction rate and the shoot differentiation rate of the rhizomes, and supplement of 100 g/L mashed bananas in medium significantly improved the shoot induction rate and the differentiation rate. Reduced the content of N (1/10N) and increased the content of P (5P) in the medium, can improved the shoot induction rate and the differentiation rate. Maltose was the most suitable carbon source for improving the shoot induction rate and the differentiation rate of the rhizomes and 20 g/L sucrose promoted the shoot differentiation of the rhizomes significantly.