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农学学报 ›› 2020, Vol. 10 ›› Issue (7): 7-14.doi: 10.11923/j.issn.2095-4050.cjas2020-0079

所属专题: 水稻

• 农艺科学/生理生化 • 上一篇    下一篇

利用重组自交系高密度遗传图谱定位水稻芽期耐冷QTL

刘睿琦1, 邹安东1, 吴光亮1,2, 王鹏1,2, 李才敬1,2, 张翔宇1,2, 程琴1,2, 王燕宁1,2, 黄诗颖1,2, 贺浩华1,2,3(), 边建民1,2,3()   

  1. 1江西农业大学/作物生理生态与遗传育种教育部重点实验室,南昌 330045
    2江西省水稻高水平工程研究中心,南昌 330045
    3江西农业大学/作物生理生态与遗传育种江西省重点实验室,南昌 330045
  • 收稿日期:2020-05-29 出版日期:2020-07-20 发布日期:2020-07-16
  • 通讯作者:
    边建民,男,1981年出生,河北张家口人,教授,博士,主要从事水稻遗传育种方面的研究。通信地址:330045 江西省南昌市青山湖区蛟桥镇江西农业大学,E-mail:
    贺浩华,男,1964年出生,江西莲花人,教授,博士,主要从事水稻遗传育种方面的研究。通信地址:330045 江西省南昌市青山湖区蛟桥镇江西农业大学,E-mail:
  • 作者简介:

    刘睿琦,男,1999年出生,江西吉安人,本科,研究方向:水稻遗传育种研究。通信地址:330045 江西省南昌市青山湖区蛟桥镇江西农业大学北区,E-mail: 1017003246@qq.com

  • 基金资助:
    江西省杰出青年人才资助计划项目“水稻芒长主效基因qAWN4的克隆与功能分析”(20192BCB23010); 江西省教育厅科技研究项目“水稻绿色三系不育系新品种(系)选育”(GJJ170241); 江西省自然科学类重点项目“控制籼稻保持系颖花形成关键基因的解析”(20192ACBL20017); 大学生创新创业训练计划项目“利用重组自交系高密度遗传图谱定位和评价水稻芽期耐冷QTL”(202010410106)

Mapping Cold-Tolerant QTL in Rice at Seedling Stage: Using a High-Density Genetic Map of Recombinant Inbred Lines

Liu Ruiqi1, Zou Andong1, Wu Guangliang1,2, Wang Peng1,2, Li Caijing1,2, Zhang Xiangyu1,2, Cheng Qin1,2, Wang Yanning1,2, Huang Shiying1,2, He Haohua1,2,3(), Bian Jianmin1,2,3()   

  1. 1Jiangxi Agricultural University/Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Nanchang 330045, Jiangxi, China
    2Jiangxi Super Rice Engineering Technology Research Center, Nanchang 330045, Jiangxi, China
    3Jiangxi Agricultural University/Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Jiangxi Province, Nanchang 330045, Jiangxi, China
  • Received:2020-05-29 Online:2020-07-20 Published:2020-07-16

摘要:

为定位水稻芽期耐冷QTL,本实验以双季超级稻品种‘五丰优T025’的双亲‘五丰B’和‘昌恢T025’杂交衍生的重组自交系(recombinant inbred lines, RILs)群体为材料,对10℃低温处理的水稻幼芽的存活率、根数、根长和芽长进行了测定。利用QTL Icimapping v4.2软件,共检测到3个控制芽期耐冷性QTL:qRL1qRL2qBL6,分别位于第1、2、6染色体上,LOD值分别为2.98,2.51和5.26,分别解释表型变异的10.54%,8.67%和14.04%,其增效等位基因均来自于亲本‘昌恢T025’。这些QTL定位在6.75k~40.05 kb染色体区间,为后续利用这些QTL进行分子标记辅助,选育芽期耐冷籼稻新品种奠定了基础。此外,检测到13对影响水稻芽期耐冷上位性互作QTL,分布在所有12条染色体,其中第3染色体与第8染色体之间互作位点可解释的表型变异率达到21.77%,表明上位性互作QTL在调控水稻芽期耐冷过程中也发挥了重要作用。

关键词: 水稻, 重组自交系, 芽期, 耐冷性, QTL分析

Abstract:

To map the cold-tolerant QTL in rice, the recombinant inbred lines (RILs) population derived from the cross between ‘Wufeng B’ and ‘Changhui T025’, the parents of double-cropping super rice ‘Wufengyou T025’, were used as the materials. The survival rate, root number, root length and bud length at young seedling stage treated at low temperature of 10℃ were determined. Three QTL, qRL1, qRL2, and qBL6 controlling the cold tolerance of rice at seedling stage, were detected by QTL Icimapping v4.2 software. These QTL were identified on chromosome 1, 2 and 6, respectively, their LOD values were 2.98, 2.51 and 5.26, respectively, and the phenotypic variation explained by these QTL were 10.54%, 8.67% and 14.04%, respectively, and the positive alleles of the three QTL were all from ‘Changhui T025’. These QTL were mapped at an interval of 6.75k-40.05kb on Nipponbare genome, which laid the foundation for the improvement of indica cold tolerance at young seedling stage by using these favorable QTL via marker-assisted selection. Furthermore, 13 pairs of epistatic interaction QTL were detected on all 12 chromosomes, and the epistatic interaction between chromosome 3 and chromosome 8 accounted for 21.77% of the phenotypic variation. The results indicate that the epistatic interaction effect plays an important role in rice cold tolerance at young seedling stage.

Key words: Rice, Recombinant Inbred Lines (RILs), Seedling Stage, Cold Tolerance, QTL Analysis

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