Abstract: [Objective] To evaluate soybean rhizosphere micro the allelopathic effect of ecological environment after different concentration of leaching solution of Equisetum arvense treatments, [Method] the changes of soil property、soil microbial quantity and soil enzyme were investigated. [Result]The results showed that the content of alkali nitrogen、available phosphorus and organic matter, the number of bacteria、fungi and actinomycetes were reduce after greater concentration of leaching solution of Equisetum arvense treatment; the activity of catalase were increase, the activity of urease were reduce, the activities of phosphatase and invertase were no apparent change after leaching solution of Equisetum arvense treatment. [Conclusion] Therefore, allelochemicals of Equisetum arvense decreased soil nutrient, and the soil enzyme is cushion for foreign substances.