Effect of Inoculated Funneliformis mosseae on Root Rot Pathogen Flora Structure in Soybean Continuous Cropping

doi:10.11923/j.issn.2095-4050.cjas15120018

Abstract

Abstract: In this study, the bioremediation method was applied to preventing the root rot in soybean continuous cropping in order to reduce the occurrence of root rot and enhance the quality of soybean. Three soybean varieties were selected and inoculated with Funneliformis mosseae in the trial. The root rot pathogen community structure of the root system and rhizosphere soil in soybean continuous cropping were measured by PCR-DGGE. The results showed that the abundance and diversity index of root rot pathogen of the soybean root system and rhizosphere soil samples in the treatment groups were significantly lower than that in the control groups after inoculated with F. mosseae, and F. mosseae had different effect on different soybean cultivars. The results of cluster analysis also verified that F. mosseae affected the occurrence and distribution of root rot pathogen in soybean continuous cropping.

CLC Number:

S435.651

References

[1] Hartman G L, West E D, Herman T K. Crops that feed the world 2.Soybean-worldwide production, use, and constraints caused by pathogens and pests [J]. Food Security, 2011, 3(1): 5–17.
[2] Han J, Jie W G, Cai, B Y. Effect of phosphorus on the composition and accumulation of 7S and 11S globulin subunits during seed development of three soybean varieties [J]. Journal Soil Science and Plant Nutrition, 2014, 14(4): 769-782.S
[3] Chen M N, Li X, Yang Q L. Dynamic succession of soil bacterial community during continuous cropping of peanut (Arachishypogaea L.) [J]. PLoS One, 2014, 9(7): e101355. doi:10.1371/journal.pone.0101355.
[4] 魏巍, 许艳丽, 朱琳, 等. 长期连作对大豆根际土壤镰孢菌种群的影响 [J]. 应用生态学报, 2014, 25(2); 497-504.
[5] Xiong W, Zhao Q Y, Zhao J, et al. Different continuous cropping spans significantly affect microbial community membership and structure in a vanilla-grown soil as revealed by deep pysosequencing [J]. Microbial Ecology, 2014, doi: 10.1007/s00248-014-0516-0.
[6] Reinhardt D. Programming good relations development of the arbuscular mycorrhial symbiosis [J]. Current Opinion in Plant Biology, 2007, 10(1): 98-105.
[7] Walder F, Niemann H, Natarajan M, et al. Mycorrhizal networks: common goods of plants shared under unequal terms of trade [J]. Plant Physiology, 2012, 159(2): 789-797.
[8] Wei Y, Hou H, Li J N, et al. Molecular diversity of arbuscular mycorrhizal fungi associated with an Mnhyperaccumulator-Phytolacca Americana, in Mn mining area [J]. AppliedSSoilSEcology, 2014, 82: 11-17.
[9] Lehmann A, Kathryn-Barto E, Powell J R, et al. Mycorrhizal responsiveness trends in annual crop plants and their wild relatives-a meta-analysis on studies from 1981 to 2010 [J]. Plant Soil, 2012, 355(1-2): 231-250.
[10]Zhang H S, Li G, Qin F F, et al. Castor bean growth and rhizosphere soil property response to different proportions of arbuscular mycorrhizal and phosphate-solubilizing fungi [J]. Ecological Research, 2014, 29(2): 181-190.
[11]Zhang F G, Zhu Z, Yang X M, et al. Trichodermaharzianum T-E5 significantly affects cucumber root exudates and fungal community in the cucumber rhizosphere [J]. AppliedSSoilSEcology, 2013, 72:41-48.
[12]Huang Y M, Srivastava A K, Zou Y N, et al. Mycorrhizal-induced calmodulin mediated changes in antioxidant enzymes and growth response of drought-stressed trifoliate orange. Frontiers in Microbiology, 2014, 5(682): doi: 10.3389/fmicb.2014.00682.
[13]罗巧玉, 王晓娟, 林双双, 等. AM真菌在植物病虫害防治中的作用机制 [J]. 生态学报, 2013, 33(19); 5997-6005.
[14]Ortiz N, Armada E, Duque E, et al. Contribution of arbuscular mycorrhizal fungi and/or bacteria to enhancing plant drought tolerance under natural soil conditions: effectiveness of autochthonous or allochthonous strains [J]. Journal of Plant Physiology, 2015, 174:87-96.
[15]Gomez-Bellot M J, Ortuno M F, Nortes P A, et al. Protective effects of Glomusiranicum var. tenuihypharum on soil and Viburnum tinus plants irrigated with treated wastewater under field conditions [J]. Mycorrhiza, 2014, doi: 10.1007/s00572-014-0621-4.
[16]Ei-Faiz A, Duponnois R, Winterton P, et al. Effect of different amendments on growing of Canna indica L. inoculated with AMF on mining substrate [J]. International Journal of Phytoremediation, 2015, 17(5): 503-513.
[17]樊宇红, 凌宏文, 朴河春. 桑树(Morus alba)与丛枝菌根的共生对重金属元素吸收的影响 [J].生态环境学报, 2014, 23(3): 477-484.
[18]杨海霞, 李士美, 郭绍霞. 丛枝菌根真菌对紫薇耐盐性的影响 [J]. 植物生理学报, 2014, 50(9): 1379-1386.
[19]周宝利, 郑继东, 毕晓华, 等. 丛枝菌根真菌对茄子黄萎病的防治效果和茄子植株生长的影响 [J]. 生态学杂志, 2015, 34(4): 1026-1030.
[20]Qian L, Yu W J, Cui J Q, et al. Funnelifomis mosseae affects the root rot pathogen Fusarium oxysporum in soybeans [J]. Acta Agriculturae Scandinavica, Section B-Soil Plant Science, 2015, 64(4): 321-321.
[21]Jie W G, Bai L, Yu W J, et al. Analysis of interspecific relationships between Funeliformis mosseae and Fusarium oxysporum in the continuous cropping of soybean rhizosphere soil during the branching period [J]. Biocontrol Science and Technology, 2015, 25(9): 1036-1051.
[22]Yergeau E, Filion M A. PCR-denaturing gradient electrophoresis approach to assess Fusarium diversity in asparagus [J]. Journal of Microbiological Methods,2005, 60: 143-154.
[23]范雪露, 崔佳琦, 蔡柏岩. 接种摩西柄囊霉对连作大豆苗期根腐病病原真菌多样性的影响 [J]. 中国农学通报, 2014, 30(31); 272-279.
[24]魏巍, 许艳丽, 张思佳, 等. 大豆根腐病原镰孢菌种群多样性DGGE分析及其致病性研究 [J].植物病理学报, 2013, 43(5); 500-508.
[25]鞠会艳, 韩丽梅, 王树起, 等. 连作大豆根系分泌物对根腐病病原真菌的化感作用[J]. 应用生态学报, 2002, 13(6); 723-727.
[26]马丹炜, 王亚男, 王煜, 等. 化感胁迫诱导植物细胞损伤研究进展 [J]. 生态学报, 2015, 35(5); 1640-1645.
[27]Jie W G, Liu X R, Cai B Y. Diversity of rhizosphere soil arbuscular mycorrhizal fungi in various soybean cultivars under different continuous cropping regimes [J]. PLoS One, 2013, 8(8): e72898.doi: 10.1371/journal.pone.0072898.