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Journal of Agriculture ›› 2021, Vol. 11 ›› Issue (10): 95-101.doi: 10.11923/j.issn.2095-4050.cjas20190600065

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Application of Triglyceride Fingerprint in Identification of Adulterated Edible Oil

Weng Jia(), Huang Xiuli, Zhu Wenjuan, Wang Xiaoqin   

  1. Huizhou Institute for Food and Drug Control, Huizhou 516003, Guangdong, China
  • Received:2019-06-03 Revised:2019-10-08 Online:2021-10-20 Published:2021-10-28

Abstract:

To construct the triglyceride fingerprint of olive oil, soybean oil, peanut oil, corn oil, sunflower oil and rapeseed oil, triglycerides of the six edible vegetable oils were isolated by HPLC-ELSD. The data was imported into the traditional Chinese medicine chromatographic fingerprint similarity evaluation system (version 2012) by the average vector method, and the similarity of the fingerprint was analyzed. The results showed that the fingerprint profile of triglycerides was similar among different brands of the same edible vegetable oil, while was significantly different among various edible vegetable oils. Olive oil had low similarity with corn oil, soybean oil, or sunflower oil, and the similarity was 0.233, 0.086 and 0.073, respectively; however, it had high similarity with rapeseed oil and peanut oil, and the similarity was 0.830 and 0.776, respectively. When olive oil was mixed with 10% corn oil, soybean oil, or sunflower oil, the similarity of triglyceride fingerprint was less than 0.90, but the similarity change was not obvious when mixed with rapeseed oil. Area normalization method was used to analyze the characteristic peak of fingerprint, and the detection limit of adulteration ratio was less than 15%. The combination of triglyceride fingerprint and area normalization method can be an effective method for identification the adulteration of edible vegetable oil.

Key words: edible vegetable oil, triglyceride, fingerprint, identification, adulteration

CLC Number: