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Journal of Agriculture ›› 2021, Vol. 11 ›› Issue (2): 74-78.doi: 10.11923/j.issn.2095-4050.cjas2020-0052

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Identification of Codonopsis pilosula Crown Rot Disease and Its Sensitivity to Fungicides

Qi Yonghong1(), Li Minquan2, Cao Sufang2, Jiang Jingjing1, Li Xueping1, Li Qingqing1, Xu Meirong3, Li Jiping1()   

  1. 1Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou 730070, Gansu, China
    2Institute of Fruit and Floriculture Research, Gansu Academy of Agricultural Sciences, Lanzhou 730070, Gansu, China
    3Institute of Agricultural Quality Standards and Testing Technology, Gansu Academy of Agricultural Sciences, Lanzhou 730070, Gansu, China
  • Received:2020-05-19 Revised:2020-09-21 Online:2021-02-20 Published:2021-02-24
  • Contact: Li Jiping E-mail:qiyonghong920@gsagr.ac.cn;lijiping@gsagr.ac.cn

Abstract:

To clarify the pathogen, pathogenicity and dominant pathogen species of Codonopsis pilosula crown rot disease and their sensitivity to different fungicides, the pathogen was isolated by tissue isolation method and identified according to its culture traits, morphology and molecular biological characteristics. Meanwhile, the sensitivity of different fungicides was determined by mycelium growth inhibition method. The results showed that the disease pathogens were identified as Fusarium oxysporum, F. sonali and F. equiseti. The dominant species was F. oxysporum. Pathogenicity testing showed that the three species could cause C. pilosula crown rot disease, F. oxysporum had the strongest pathogenicity, and its disease rate and disease index had a significant difference from those of the other two. Laboratory toxicity test of different fungicides to the mycelia growth of the three species showed that difenoconazole fungicide had the strongest inhibition activity, and the three Fusarium species had the highest sensitivity to pyraclostrobin fungicide.

Key words: Codonopsis pilosula, Crown Rot Disease, Fusarium spp., Pathogen Identification, Fungicides, Sensitivity Testing

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