RNAi Vector Construction of Lilium GBSS Gene

doi:10.11923/j.issn.2095-4050.cjas2020-0211

Abstract

Abstract:

To study the mechanism of GBSS gene affecting the synthesis and metabolism of bulb starch during the formation and development of lily bulbs, a RNAi vector interfering with GBSS gene was constructed to provide materials for GBSS silencing expression at the transcriptional level. The conserved sequence of 300 bp of GBSS gene was cloned from lily scales. The sequence was positively and inversely connected to the interference vector pJawohl8-RNAi by BP and LR reaction. pDONR221-GBSS entry clone and pJawohl8-RNAi-GBSS expression vector were successfully constructed, which could provide materials and reference for the study of GBSS gene function and its influence on bulb development in the synthesis and metabolism pathway of lily bulb starch.

Key words: Lilium, GBSS gene, bulb starch, metabolism, mechanism, granule-bound starch synthase, RNAi, expression vector, bulb development

CLC Number:

Q812

ZHANG Jinzhong. RNAi Vector Construction of Lilium GBSS Gene[J]. Journal of Agriculture, 2022, 12(7): 69-73.

Figures/Tables 5

References 20

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