Abstract:

The aims are to ensure the success of cultured bombyx batryticatus by artificial feeding, establish the method and quality assessment index of bacteria for bombyx batryticatus. Bacteria of bombyx batryticatus from different sources were tested. The blood counting chamber was used for counting sprouted spore and flat colony counting method was applied to determine the number of mixed fungi and Beauveria bassiana. Both the number of sprouted active spore and active spore in plate of bombyx batryticatus bacteria made by liquid-solid dual-phase fermentation were greater than 10¹⁰ spore/g, while the number of mixed fungi was less than 10⁵ CFU/g, and the number of mixed bacteria was less than 10⁴ CFU/g. Meanwhile, both the number of sprouted active spore and active spore in plate of bombyx batryticatus bacteria produced by nature were less than 10¹⁰ spore/g, while the number of mixed fungi was greater than 10⁵ CFU/g. and the number of mixed bacteria was greater than 10⁸ CFU/g. The test method for bombyx batryticatus bacteria is feasible. The index of bombyx batryticatus bacteria made by liquid-solid dual-phase fermentation is better than that by nature, and could be popularized in the culture base of bombyx batryticatus by artificial feeding.

Key words: bombyx batryticatus, liquid-solid dual-phase fermentation, Beauveria bassiana spore, the number of sprouted spore, the number of mixed fungi and mixed bacteria