Effect of β-glucanase from Trichoderma on Pepper Growth and Resistance to Bacterial Wilt Disease

doi:10.11923/j.issn.2095-4050.cjas2025-0011

Abstract

Abstract:

The purpose of this study is to investigate the growth promoting effect of β-glucanase from Trichoderma harzianum on pepper seedlings and its biological control effect on pepper wilt disease, in order to provide new reference directions for the biological disease control. Pepper seedlings were treated with different dilutions of β-glucanase solution, and after 30 days of transplantation, various growth indicators of peppers were detected and analyzed. Artificial inoculation of pathogenic bacteria was used to study the prevention and control effect of β-glucanase on potted pepper bacterial wilt disease. The incidence of pepper wilt disease during the seedling stage, initial flowering stage, and fruit setting stage was investigated, and the field control effect of β-glucanase on pepper wilt disease was explored. The results showed that the 10 fold solution of β-glucanase had a growth promoting rate of over 80% on the stem diameter, aboveground fresh mass, and underground fresh mass of pepper seedlings, and effectively improved the seedling strength index of pepper seedlings. The treatment of β-glucanase solution had a significant inhibitory effect on the occurrence and prevalence of pepper bacterial wilt. In the fruit setting stage of pepper, the incidence rate of β-glucanase solution treatment with different dilution ratios was 9.33%-15.00% lower than that of water control, and the control effect was 42.11%-68.22% respectively. β-glucanase from Trichoderma has a certain degree of growth promoting effect on pepper seedlings and significantly improves their resistance to bacterial wilt disease. β-glucanase has the potential to be developed into a biological agent for pepper fitness cultivation and prevention of pepper wilt disease.

Key words: β-glucanase, pepper, promoting effect, bacterial wilt disease, biocontrol effect

YANG Chunlin, YANG Zhigang, HU Qiang, XI Yadong, LI Honghao, YANG Ziwei, HAO Lining, YAN Xuehai, TU Rui, YANG Yunmei. Effect of β-glucanase from Trichoderma on Pepper Growth and Resistance to Bacterial Wilt Disease[J]. Journal of Agriculture, 2026, 16(4): 12-19.

Figures/Tables 7

References 40

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级数	症状描述
0	无症状
1	1片叶萎蔫
2	2~3片叶萎蔫
3	除顶部2~3片叶外，其他叶片都萎蔫
4	整株叶片全部萎蔫

级数	症状描述
0	无症状
1	1片叶萎蔫
2	2~3片叶萎蔫
3	除顶部2~3片叶外，其他叶片都萎蔫
4	整株叶片全部萎蔫

处理	株高/cm	促生率/%	基部茎粗/mm	促生率/%	地上鲜质量/g	促生率/%
10倍液	26.74±0.53a	28.43	3.92±0.07a	83.18	5.34±0.19a	105.38
25倍液	25.66±0.68a	23.25	3.39±0.15b	58.41	5.11±0.15a	96.54
50倍液	23.70±0.19b	13.83	2.67±0.13c	24.77	4.17±0.20b	60.38
100倍液	21.53±0.63c	3.41	2.38±0.15cd	11.21	3.45±0.22c	32.69
清水对照	20.82±0.26c	-	2.14±0.20d	-	2.60±0.17d	-

处理	株高/cm	促生率/%	基部茎粗/mm	促生率/%	地上鲜质量/g	促生率/%
10倍液	26.74±0.53a	28.43	3.92±0.07a	83.18	5.34±0.19a	105.38
25倍液	25.66±0.68a	23.25	3.39±0.15b	58.41	5.11±0.15a	96.54
50倍液	23.70±0.19b	13.83	2.67±0.13c	24.77	4.17±0.20b	60.38
100倍液	21.53±0.63c	3.41	2.38±0.15cd	11.21	3.45±0.22c	32.69
清水对照	20.82±0.26c	-	2.14±0.20d	-	2.60±0.17d	-

处理	根长/mm	促生率/%	根体积/mm³	促生率/%	地下鲜质量/g	促生率/%
10倍液	11.29±0.63a	34.09	242.64±5.32a	75.53	0.92±0.05a	80.39
25倍液	9.46±0.32b	12.35	192.09±4.92b	38.96	0.75±0.04b	47.06
50倍液	8.90±0.43b	5.70	154.38±4.88c	11.68	0.62±0.01c	21.57
100倍液	8.70±0.68b	3.33	151.61±7.81cd	9.68	0.61±0.04c	19.61
清水对照	8.42±0.30b	-	138.23±4.44d	-	0.51±0.02c	-