Abstract:

 In order to gain the knowledge of genetic diversity of wheat varieties with resistance to wheat midge (Sitodiplosis mosellana), on the basis of field insect plot appraisal, 47 wheat varieties/lines were involved by 19 SSR markers to explore their genetic diversity. The 19 SSR markers were screened and a total of 104 allelic variants were detected in 47 wheat varieties. Three to eight alleles per marker were detected, with an average of 5.47. The means of alleles of high resistant, moderate resistant, sensitive and high sensitive varieties were 4.11, 4.68, 3.95 and 3.84, respectively. The PIC (polymorphism information content) for each polymorphic primer was varied from 0.35 to 0.86 with an average of 0.74. The PICs of high resistant, moderate, sensitive and high sensitive varieties had an average of 0.64 (0.31~0.81), 0.69 (0.38~0.88), 0.67 (0.40~0.80) and 0.67 (0.20~0.89), respectively. The genetic distance (GD) of 47 varieties was ranged from 0.40 to 0.95, with an average of 0.71. The GDs of high resistant, moderate resistant and high sensitive varieties were varied from 0.40 to 0.95, from 0.46 to 0.90 and from 0.41 to 0.90, with an average of 0.66, 0.69 and 0.68. Based on cluster analysis results, the 47 varieties were clustered into six groups. Resistant variety Jinmai 65 was a group by oneself, indicating that there was a farther genetic relationship between Jinmai 65 and other varieties, and so the Jinmai 65 might be used as resistant parent and might be popularized in production.