Abstract: To analyze the relationship between twenty serum biochemical indexes and Oxytropis glabra DC poisoning in Hetian Sheep, and to provide the basis for the clinical diagnosis and treatment of Oxytropis glabra DC poisoning in animals. The activities of AST (aspertate aminotransferase), ALT (alanine aminotransferase), AKP (alkaline phosphatase), LDH (lactate dehydrogenase), AMA (α-mannosidase)and concentration of BUN (blood urea nitrogen), CR(creatinine), GLU(glucose), TG(triglyceride), TC(total cholesterol), TP(total protein), ALB(albumin), GLO(globulin), HDL-C(high-density lipoprotein cholesterol), LDL-C(low-density lipoprotein cholesterol), VLDL-C(very low-density lipoprotein cholesterol) were detected in sera of 6 sheep cases poisoning of Oxytropis glabra DC and 8 normal sheep as control. The t test was used to analyze differences of the biochemical indexes between the poisoning sheep and control, then the simple correlation method was used to analyze the relationship between indexes and poisoning by Oxytropis glabra DC. The results demonstrated that an increase of AST, ALT, AKP, LDH, BUN, CR, GLU, TC, GLO, LDL-C and VLDL-C in poisoning group, while a decrease of AMA, TG and ALB in poisoning group, with significant differences between the experimental groups (P＜0.05 or P＜0.01), the content of TP and HDL-C were not significantly lower than control group(P＞0.05). The main indexes relating with Oxytropis glabra DC poisoning(OgP) disease were the activity of AMA, GLU, LDH, AKP, TG and CR, and the stepwise regression equation was as follows: OgP=0.681-0.073AMA+0.055GLU+0.036LDH+0.041AKP. The biochemical indexes including the concentrations of AMA, GLU, LDH, GLO, TG and CR were of great importance in the early clinical diagnosis, treatment, curative effect observation and outcome judgments of Oxytropis glabra DC poisoning in Hetian Sheep.