Abstract: The purpose of this study was to clone the chalcone synthase gene from Rosa rugosa and conduct bioinformatics analysis of the gene related information, and to lay a good foundation for changing Rosa rugosa’s color and breeding new varieties by genetic engineering. In this work, a full- length cDNA sequence of chalcone synthase (CHS) gene was obtained from petals of Rosa rugosa‘Tanghong’using RT-PCR and RACE. With the bioinformatics analysis, the cloned CHS gene was 1416 bp in full length, containing 1167 bp opening reading frame (ORF), which encoded 389 amino acids, the predicted CHS protein’s molecular formula was C1899H3043N505O562S18, whose relative molecular mass was 42.518 kDa and isoelectric point was 6.12. The CHS protein’s instability index was computed to be 37.45, which classified the protein as stable. And The CHS protein had no signal peptide and splice site. Characteristic prediction results indicated that the secondary structure of CHS protein mainly included 42.16%α -helix, 29.05% random coil, 10.54%β -sheet and 18.25% extended chain. Phylogenetic analysis indicated that the cloned CHS gene shared the closest homology with the CHS from Rosa chinensis and Fragaria×ananassa, and had farther homology with other plants. The CHS gene was cloned for the first time, this study obtained its bioinformatics information, proved the importance of CHS gene in flavonoids biosynthesis, provided a theoretical basis for improving Rosa rugosa’s color.