Abstract: To further optimize the industrial cultivation condition of Pleurotus eryngii, and provide suitable species for factory production, 16 Pleurotus eryngii cultivars from different places were used as materials. The mycelium growth, the rate of mycelium growth and the index of mycelium growth was compared, and the growth characteristics of different parent hypha species were studied. The results showed that the mycelium growth of three cultivars, No.4 ‘( apricot farmers No.6’), No.8 ‘( Pleurotus eryngii No.79’), No.15 ‘( Ao Kun’), was uniform dense and well, and the average growth rate was fast, 0.77 cm/d, which was higher than those of other cultivars. The index of mycelium growth was the largest, up to 3.86. Therefore, considering the characteristics of mycelium growth, No.4 (apricot farmers No.6), No.8 (Pleurotus eryngii No.79) and No.15 (Ao kun) could be used as excellent strains of Pleurotus eryngii cultivation.