Abstract: To understand the change of lignin content and endocarp lignin deposition process in different periods of different cultivars,‘Jinsixiaozao’and‘Jinsiwuhexiaozao’were used as the test materials, the lignin content in jujube fruit was measured by the Klason method and the distribution of lignin was observed by color reaction between the phloroglucinol and lignin for the purpose of researching the dynamic changes of lignin deposition in jujube fruit. The results showed that: in jujube fruit the endocarp lignin deposition process was from the base, after the lignification of medial peel was completed, the lignification was achieved from the inside to the outside until gradually accomplished; during 15-25 days after flowering, the lignin content in ‘Jinsixiaozao’and‘Jinsiwuhexiaozao’fruit was basically the same, and starting from the 40 days after flowering, the lignin content in‘Jinsixiaozao’fruit began to rise and exceeded the lignin content in ‘Jinsiwuhexiaozao’fruit; the change of the lignin content in‘Jinsiwuhexiaozao’fruit was smooth within all fruit development period, and that in‘Jinsixiaozao’fruit increased obviously during 15-75 days, after 75 days the lignin content showed a leap in growth and increased more significantly. There are significant differences in the process of lignification between the two cultivars of jujube in the experiment, so they can be used for related research on jujube fruit endocarp lignification.