Abstract: Davidia involucrata is an endangered ancient relic species. As the flower shape of Davidia involucrata is graceful, it has great application value in gardening. But Davidia involucrata has obstacles in seed and shoot cutting propagation. Therefore, it is urgent to develop the technology of plant tissue culture for establishing a fast propagation system according to the needs of both germplasm conservation and economic application. In this research, explant selection, different concentrations of mercuric chloride disinfectant and basal culture media were explored. The results showed that spring bud and semi-lignified branches with bud were the best explants, and 75% alcohol disinfection for 10 s and followed by 0.1% HgCl2 disinfection for 10 min could meet the need, and the WPM basal media had the best effect. The results built the bud proliferation technique system, but the rooting of cultured seedlings need to be further studied.