Journal of Agriculture ›› 2020, Vol. 10 ›› Issue (5): 31-35.doi: 10.11923/j.issn.2095-4050.cjas20190600089
Special Issue: 小麦
• Research article • Previous Articles Next Articles
Zhou Huafei1(), Wu Jiawen2, Yang Hongfu1, Chen Hongzhou1, Shu Zhaolin1, Rao Bin1, Zhu Jianfei1, Yao Kebing1()
Received:
2019-06-21
Revised:
2019-07-10
Online:
2020-05-15
Published:
2020-05-16
CLC Number:
Zhou Huafei, Wu Jiawen, Yang Hongfu, Chen Hongzhou, Shu Zhaolin, Rao Bin, Zhu Jianfei, Yao Kebing. Fusarium spp. in Jiangsu: Species Detection and Resistance Analysis[J]. Journal of Agriculture, 2020, 10(5): 31-35.
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URL: http://nxxb.caass.org.cn/EN/10.11923/j.issn.2095-4050.cjas20190600089
病原菌 | 扩增条件 | 灵敏度 | 引物序列(5?-3?) |
---|---|---|---|
F. graminearum | 62℃ 60 min | 100 | F3:GTCCAATCCACTCCATCCTC[ |
B3:CGGTCTTCTCGAGAGGTTCA | |||
FIP:GTGTGTGGGATGGTGGCACTACAAGTCAAATCACCCATGCG | |||
BIP:CTTTCGTCTCCCGGCACAATGGTCTTGTCCCAACGATGAGGA | |||
LB:CCCGCTCAGAAGAATTCTTCC | |||
F. asiaticum | 63℃ 60 min | 70 | F3:GAGACTGGTAATCAAGGTGATGA[ |
B3:CGTACAATTCCTGAACCAGATGA | |||
FIP:GTGATCAGGAAGAGGTTCCCCATCGACGGATATGGTTTGGACGCTT | |||
BIP:GCTGATCGCCATCCTGATGGGGGCTTATGAGCCAGATTGAGAA | |||
LB:GCCGCTAGTGATGCTTGGCTTA |
病原菌 | 扩增条件 | 灵敏度 | 引物序列(5?-3?) |
---|---|---|---|
F. graminearum | 62℃ 60 min | 100 | F3:GTCCAATCCACTCCATCCTC[ |
B3:CGGTCTTCTCGAGAGGTTCA | |||
FIP:GTGTGTGGGATGGTGGCACTACAAGTCAAATCACCCATGCG | |||
BIP:CTTTCGTCTCCCGGCACAATGGTCTTGTCCCAACGATGAGGA | |||
LB:CCCGCTCAGAAGAATTCTTCC | |||
F. asiaticum | 63℃ 60 min | 70 | F3:GAGACTGGTAATCAAGGTGATGA[ |
B3:CGTACAATTCCTGAACCAGATGA | |||
FIP:GTGATCAGGAAGAGGTTCCCCATCGACGGATATGGTTTGGACGCTT | |||
BIP:GCTGATCGCCATCCTGATGGGGGCTTATGAGCCAGATTGAGAA | |||
LB:GCCGCTAGTGATGCTTGGCTTA |
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