Welcome to Journal of Agriculture,

Journal of Agriculture ›› 2020, Vol. 10 ›› Issue (7): 19-23.doi: 10.11923/j.issn.2095-4050.cjas19030017

Special Issue: 生物技术 小麦

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Activity of α-amylase in Wheat Seed Germination at Different Altitudes in Qinghai: Comparison

Wang Li1,2, Quan Youjuan1,3, Li Xiang1,3, Liu Demei1,4,5, Chen Zhiguo1,4,5()   

  1. 1Northwest Institute of Plateau Biology, CAS, Xining 810008, Qinghai, China
    2Qinghai University, Xining 810016, Qinghai, China
    3University of Chinese Academy of Science, Beijing 100049, China
    4Key Laboratory of Adaptation and Evolution of Plateau Blota, CAS, Xining 810008, Qinghai, China
    5Qinghai Provincial Key Laboratory of Crop Molecular Breeding, Xining 810008, Qinghai, China
  • Received:2019-03-14 Online:2020-07-20 Published:2020-07-16

Abstract:

The purpose is to explore the reason why the germination rate of spring wheat in Xiangride Area, Dulan County (in high altitude Qaidam Basin) was significantly lower than that of Ledu Area (low altitude) in the East of Qinghai. In this experiment, four wheat varieties from the two areas were used. The TP germination paper method was used to simulate field germination environment by artificial climate chamber. The α-amylase activity was measured and compared during the 7th day germination period. The germination rate of wheat seeds in low altitude area was significantly higher than that in high altitude area, the α-amylase activity of seeds of the four varieties in Ledu Area was significantly higher than that in Xiangride Area, which could be one of the reasons that the germination rate of wheat seed in Xiangride area was lower than that in Ledu area. The germination rate of wheat seeds is affected by a series of factors, such as moisture content, grain maturation stage and storage condition. At the same time, a series of enzymes are needed in wheat germination, among which α-amylase plays an important role in wheat germination.

Key words: Qinghai Province, Wheat, Germination Rate, α-amylase, Enzyme Activity

CLC Number: