Welcome to Journal of Agriculture,

Journal of Agriculture ›› 2022, Vol. 12 ›› Issue (1): 60-64.doi: 10.11923/j.issn.2095-4050.cjas20200200029

Special Issue: 生物技术 园艺

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Study on Tissue Culture and Rapid Propagation of Tilia amurensis

LI Qinghua1(), WANG Yinhua1(), KONG Yuguang2, YAO Junxiu1, LU Yizeng3, YAN Liping1, WU Dejun1(), REN Fei1, GE Wenhua4   

  1. 1Shandong Academy of Forestry Sciences/Shandong Key Laboratory of Forest Tree Genetic Improvement, Jinan 250014, Shandong, China
    2Shandong Territorial and Spatial Planning Institute, Jinan 250014, Shandong, China
    3Shandong Forest Germplasm Resources Center, Jinan 250014, Shandong, China
    4Dashawa Forest Farm of Rizhao, Rizhao 276825, Shandong, China
  • Received:2020-02-20 Revised:2021-01-20 Online:2022-01-20 Published:2022-02-24
  • Contact: WANG Yinhua,WU Dejun E-mail:jinanlinye@163.com;273068070@qq.com;sdlky412@163.com

Abstract:

To explore the establishment of tissue culture and rapid propagation system of Tilia amurensis, the effects of basic medium, plant growth regulator concentration and ratio on tissue culture and rapid propagation of T. amurensis were studied. The results show that 1/2MS medium is the most suitable medium for proliferation, and the proliferation coefficient could reach 13.5 when 0.05 mg/L 6-BA and 0.03 mg/L IBA are added. MS medium is the most suitable medium for strong seedling and rooting, the best medium for strong seedling is MS+ 0.1 mg/L 6-BA+ 0.1 mg/L IBA+ 0.03 mg/L GA3, with an average seedling height of 5.15 cm. The best rooting medium is MS+ 1.0 mg/L 6-BA+ 0.05 mg/L NAA, the rooting rate is 93.3%, and the number of roots is 5.7.

Key words: Tilia amurensis, Tissue Culture, Medium for Strong Seedling, Medium for Rooting, Vegetative Propagation, Rapid Propagation

CLC Number: