Effects of Base Fertilizer Added Microbial Agent and Seedling-raising Fertilizer on Growth and Nutrient Accumulation of Flue-cured Tobacco

doi:10.11923/j.issn.2095-4050.cjas2023-0183

Abstract

Abstract:

To clarify the effect of adding microbial agents in base fertilizer and urea in seedling-raising fertilizer on promoting early growth and rapid development of paddy-tobacco, ‘Yunyan 87’ was used as material, the two-factor experiment was designed by two application methods of base fertilizer with or without adding microbial agent, and three application methods of a single application of seedling-raising fertilizer for tobacco, a single application of urea, seedling-raising fertilizer for tobacco and urea mixed application. The effects of microbial agent, seedling-raising fertilizer and their interaction on root systems, agronomic traits, SPAD value, dry matter accumulation and nitrogen, phosphorus and potassium accumulation of flue-cured tobacco were studied. The results showed that the addition of microbial agent and seedling-raising fertilizer combined with urea was better than the single application of seedling-raising fertilizer and base fertilizer, root activity, root total length, surface area, average diameter, volume and root tip number were increased by 28.13%, 5.41%, 1.30%, 17.23%, 24.63% and 0.41%, respectively. Plant height, stem girth, number of leaves and maximum leaf area were increased by 10.06%, 2.30%, 18.75% and 37.26%, respectively. Total dry matter, nitrogen, phosphorus and potassium accumulation were increased by 27.18%, 37.21%, 36.57% and 23.97%, respectively. The addition of microbial agent and seedling-raising fertilizer combined with urea in the base fertilizer could improve the root activity of flue-cured tobacco, promote the root development of flue-cured tobacco, improve agronomic properties, facilitate the accumulation of flue-cured tobacco dry matter and nitrogen, phosphorus and potassium nutrients, promote the early growth and rapid development of flue-cured tobacco, and lay a foundation for the high quality and high yield of ‘Yunyan 87’ in tobacco-rice rotation area.

Key words: microbial agent, seedling-raising fertilizer, tobacco growth, dry accumulation and distribution, nutrient accumulation

PENG Yingchun, TAN Zhipeng, JIANG Zhimin, WU Chujie, WU Wenxin, ZONG Yi, LI Sijun, WEN Yongcai, CHENG Mingzhu, XIA Bing, DENG Xiaohua. Effects of Base Fertilizer Added Microbial Agent and Seedling-raising Fertilizer on Growth and Nutrient Accumulation of Flue-cured Tobacco[J]. Journal of Agriculture, 2024, 14(8): 38-43.

Figures/Tables 4

References 23

[1]	邓小华, 肖汉乾. 烤烟生产氮肥减施增效理论与模式[M]. 北京: 中国农业科学技术出版社, 2023.
[2]	张阳, 王新月, 谢会雅, 等. 烤烟生长对水溶性追肥配施促根剂的响应[J]. 核农学报, 2023, 37(5):1030-1039. doi: 10.11869/j.issn.1000-8551.2023.05.1030
[3]	曹健, 邓小华. 郴州浓香型特色优质烟叶生产与开发[M]. 长沙: 湖南科学技术出版社, 2016.
[4]	罗井清, 邓小华, 陈金, 等. 凹形双垄侧覆膜对耕层水热环境和上部烟叶生长及质量的影响[J]. 土壤, 2019, 51(5):1013-1019.
[5]	龚嘉, 郑重谊, 刘勇军, 等. 粉垄深耕结合开沟排水对土壤养分和烤烟生长发育的影响[J]. 西北农林科技大学学报(自然科学版), 2022, 50(2):75-81+89.
[6]	彭润润, 李举旭, 郑好, 等. 增施不同促根剂对皖南烤烟生长和品质的影响[J]. 西北农林科技大学学报(自然科学版), 2022(5):1-10.
[7]	黄琼慧, 肖汉乾, 肖艳松, 等. 稻茬烤烟减氮配施腐植酸碳肥的效应[J]. 湖南农业大学学报(自然科学版), 2022, 48(5):520-527.
[8]	齐永杰, 徐文兵, 邓小华, 等. 根区施用不同生物有机肥对稻茬烤烟生长和产质量的影响[J]. 烟草科技, 2018, 51(5):24-31.
[9]	徐赛, 张锦韬, 盘文政, 等. 微生物菌剂不同施肥方法对烤烟生长及烟叶产质量的影响[J]. 安徽农业科学, 2022, 50(21):166-169.
[10]	刘东阳, 江连强, 陈树鸿, 等. 微生物菌剂与不同有机肥协同作用对烟草根结线虫病的防控效果研究[J]. 植物医生, 2021, 34(2):23-28.
[11]	黄琼慧, 张阳, 谢会雅, 等. 水溶性追肥配施促根剂对烤烟产量和质量的影响[J]. 安徽农业大学学报, 2023, 50(2):219-227.
[12]	陈治锋, 肖汉乾, 邓小华, 等. 促根减氮施肥模式对烤烟产量和品质的影响[J]. 湖南农业大学学报(自然科学版), 2023, 49(1):12-17.
[13]	侯建林, 李思军, 何铭钰, 等. 不同施肥模式对稻茬烤烟生长和干物质积累及产质量的影响[J]. 中国农学通报, 2022, 38(33):39-43. doi: 10.11924/j.issn.1000-6850.casb2021-0720
[14]	闫鼎, 蔡宪杰, 宋明健, 等. 昭阳烟区不同配方的肥料减施技术对烤烟的影响[J]. 贵州农业科学, 2021, 49(9):53-58.
[15]	邓小华, 邓永晟, 刘勇军, 等. 垂直深旋耕配施改土物料改良酸性土壤并提高烟草种植效益研究[J]. 中国烟草学报, 2021, 27(1):64-73.
[16]	李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2003:167-169.
[17]	孙阳阳, 靳志伟, 黄明迪, 等. SPAD值与鲜烟叶成熟度及烤后烟叶质量的关系[J]. 中国烟草科学, 2016, 37(2):42-46.
[18]	邓全, 江连强, 拓阳阳, 等. 微生物菌剂与不同有机肥对烟草青枯病的协同防控效果研究[J]. 植物医生, 2021, 34(3):13-18
[19]	张瑞福, 颜春荣, 张楠, 等. 微生物肥料研究及其在耕地质量提升中的应用前景[J]. 中国农业科技导报, 2013, 15(5):8-16.
[20]	张亮, 王明霞, 张薇, 等. 外生菌根真菌对土壤钾的活化作用[J]. 微生物学报, 2014, 54(7):786-792.
[21]	柳太卫, 杨承, 李正, 等. 不同提苗措施对烤烟大田生长及品质的影响[J]. 河南农业科学, 2012, 41(7):50-53.
[22]	白建保, 李军民, 刘卫华, 等. 施氮量与移栽时穴施化肥种类对中烟100产质量的影响[J]. 烟草科技, 2005(10):33-35.
[23]	沈宏, 曹志洪. 饼肥与尿素配施对烤烟生物性状及某些生理指标的影响[J]. 土壤肥料, 1998(6):14-16.

因子	根系活性/[mg/(g·h)]	根总长度/cm	根表面积/cm²	根平均直径/mm	根体积/cm³	根尖数
A₁	0.40±0.10a	9460.23±163.53a	3755.61±83.46a	7.98±2.40a	127.38±3.34a	29087.50±694.25a
A₂	0.34±0.05b	8777.51±121.67b	3340.02±84.66b	7.48±3.07a	102.03±2.32b	25849.67±623.24b
B₁	0.36±0.11a	8941.19±110.21b	3441.07±146.79b	7.11±2.52b	91.53±3.11c	26060.33±994.46b
B₂	0.31±0.03b	8754.74±191.49c	3450.85±69.38b	7.91±3.29b	117.51±31.67b	25884.33±673.50a
B₃	0.37±0.07a	10292.94±167.15a	3895.07±77.82a	9.15±2.33a	132.87±3.35a	31404.67±600.23a
A₁B₁	0.39±0.15a	6204.86±204.97c	2303.85±35.99c	6.82±0.09c	80.48±1.10c	17681.67±584.10d
A₁B₂	0.30±0.01b	10751.22±187.03b	4239.84±40.06a	10.91±0.19a	136.34±2.29a	32238.67±590.25b
A₁B₃	0.41±0.01a	11255.27±206.06a	4333.85±36.56a	11.02±0.12a	140.32±2.04a	34579.00±142.66a
A₂B₁	0.32±0.02b	10677.53±85.76b	4278.29±71.52a	9.40±0.31b	112.59±1.96b	34439.00±630.71a
A₂B₂	0.32±0.04b	6758.27±117.57c	2661.85±48.73c	6.90±0.08c	88.67±2.93c	19530.00±305.09d
A₂B₃	0.33±0.06b	9330.62±162.32b	3656.29±57.11b	10.27±0.2a	135.41±2.12a	28230.33±932.32c

因子	根系活性/[mg/(g·h)]	根总长度/cm	根表面积/cm²	根平均直径/mm	根体积/cm³	根尖数
A₁	0.40±0.10a	9460.23±163.53a	3755.61±83.46a	7.98±2.40a	127.38±3.34a	29087.50±694.25a
A₂	0.34±0.05b	8777.51±121.67b	3340.02±84.66b	7.48±3.07a	102.03±2.32b	25849.67±623.24b
B₁	0.36±0.11a	8941.19±110.21b	3441.07±146.79b	7.11±2.52b	91.53±3.11c	26060.33±994.46b
B₂	0.31±0.03b	8754.74±191.49c	3450.85±69.38b	7.91±3.29b	117.51±31.67b	25884.33±673.50a
B₃	0.37±0.07a	10292.94±167.15a	3895.07±77.82a	9.15±2.33a	132.87±3.35a	31404.67±600.23a
A₁B₁	0.39±0.15a	6204.86±204.97c	2303.85±35.99c	6.82±0.09c	80.48±1.10c	17681.67±584.10d
A₁B₂	0.30±0.01b	10751.22±187.03b	4239.84±40.06a	10.91±0.19a	136.34±2.29a	32238.67±590.25b
A₁B₃	0.41±0.01a	11255.27±206.06a	4333.85±36.56a	11.02±0.12a	140.32±2.04a	34579.00±142.66a
A₂B₁	0.32±0.02b	10677.53±85.76b	4278.29±71.52a	9.40±0.31b	112.59±1.96b	34439.00±630.71a
A₂B₂	0.32±0.04b	6758.27±117.57c	2661.85±48.73c	6.90±0.08c	88.67±2.93c	19530.00±305.09d
A₂B₃	0.33±0.06b	9330.62±162.32b	3656.29±57.11b	10.27±0.2a	135.41±2.12a	28230.33±932.32c

因子	SPAD值	株高/cm	茎围/cm	叶片数/片	最大叶面积/cm²
A₁	29.34±2.71a	101.20±7.25a	8.93±0.46a	18.50±1.09a	1326.29±108.72a
A₂	27.87±2.93a	91.25±3.06b	8.85±0.22a	17.25±1.14a	1082.59±188.69b
B₁	28.32±1.82a	99.32±9.95a	9.05±0.48a	17.33±1.51a	1144.46±175.76b
B₂	27.28±0.55a	98.65±6.70a	8.78±0.45a	17.50±1.52a	1156.15±226.99b
B₃	28.67±2.93a	99.58±7.78a	8.78±0.17a	18.17±1.17a	1344.28±89.93a
A₁B₁	29.74±1.14a	107.20±6.97a	9.40±0.46a	18.67±0.58ab	1297.43±11.14b
A₁B₂	27.14±0.81a	104.50±1.50ab	8.63±0.65a	18.00±2.00ab	1349.71±88.22a
A₁B₃	28.40±0.90a	100.63±6.33b	8.90±0.10a	19.00±1.00a	1360.90±55.00a
A₂B₁	28.89±0.94a	91.43±3.51c	8.70±0.10a	16.00±0.00b	991.50±83.13c
A₂B₂	27.41±0.23a	92.80±2.70c	8.93±0.15a	17.00±1.00ab	962.59±92.89c
A₂B₃	27.91±1.68a	98.53±1.17b	8.67±0.15a	17.33±0.58ab	1327.65±127.92a

因子	SPAD值	株高/cm	茎围/cm	叶片数/片	最大叶面积/cm²
A₁	29.34±2.71a	101.20±7.25a	8.93±0.46a	18.50±1.09a	1326.29±108.72a
A₂	27.87±2.93a	91.25±3.06b	8.85±0.22a	17.25±1.14a	1082.59±188.69b
B₁	28.32±1.82a	99.32±9.95a	9.05±0.48a	17.33±1.51a	1144.46±175.76b
B₂	27.28±0.55a	98.65±6.70a	8.78±0.45a	17.50±1.52a	1156.15±226.99b
B₃	28.67±2.93a	99.58±7.78a	8.78±0.17a	18.17±1.17a	1344.28±89.93a
A₁B₁	29.74±1.14a	107.20±6.97a	9.40±0.46a	18.67±0.58ab	1297.43±11.14b
A₁B₂	27.14±0.81a	104.50±1.50ab	8.63±0.65a	18.00±2.00ab	1349.71±88.22a
A₁B₃	28.40±0.90a	100.63±6.33b	8.90±0.10a	19.00±1.00a	1360.90±55.00a
A₂B₁	28.89±0.94a	91.43±3.51c	8.70±0.10a	16.00±0.00b	991.50±83.13c
A₂B₂	27.41±0.23a	92.80±2.70c	8.93±0.15a	17.00±1.00ab	962.59±92.89c
A₂B₃	27.91±1.68a	98.53±1.17b	8.67±0.15a	17.33±0.58ab	1327.65±127.92a

因子	干物质积累总量/(g/株)	干物质量积累量/(g/株)			干物质分配比例/(%)
因子	干物质积累总量/(g/株)	根	茎	叶	根	茎	叶
A₁	160.86±15.27a	39.85±4.69a	42.06±6.88a	78.94±3.01a	24.77±4.19a	26.15±4.89a	49.07±1.02a
A₂	146.06±6.74b	35.36±1.93b	37.51±3.18b	73.19±2.13b	24.21±3.17a	25.68±3.83a	50.11±1.18a
B₁	145.63±9.13b	36.12±2.36b	39.82±5.24b	69.69±2.98b	24.80±1.01a	27.34±0.88	47.85±2.34a
B₂	163.76±11.92a	39.89±3.71a	45.58±5.07a	78.29±4.60a	24.36±2.76a	27.83±3.26aa	47.81±3.82a
B₃	161.23±15.57a	39.87±5.77a	40.71±0.91b	80.65±3.44a	24.73±2.38a	25.25±3.01a	50.02±1.34a
A₁B₁	153.55±2.88b	36.67±1.71b	44.58±0.56b	72.30±1.04c	23.88±1.28a	29.03±1.62a	47.09±0.83a
A₁B₂	173.92±3.07a	43.03±1.67a	50.18±0.63a	80.71±1.27b	24.74±1.07a	28.85±1.06a	46.41±1.09a
A₁B₃	175.14±4.27a	45.01±1.51a	41.43±0.52b	88.70±3.49a	25.70±0.52a	23.66±4.95a	50.65±1.40a
A₂B₁	137.71±3.46c	35.57±3.17b	35.06±0.44c	67.08±0.79d	25.83±1.09a	25.46±2.87a	48.71±0.99a
A₂B₂	153.59±5.98b	36.75±1.42b	40.97±0.51b	75.87±5.81c	23.93±0.99a	26.67±1.86a	49.40±0.84a
A₂B₃	147.31±2.70bc	34.73±1.25b	39.99±0.50b	72.60±4.00c	23.58±1.05a	27.15±3.84a	49.28±0.88a