Abstract: In order to analyze the differences on chilling tolerance of different Lagerstroemia indica L. varieties, the conductometry method , chlorophyll fluorescence method and NMT method were employed to measure various chilling tolerance parameters and their variation regularities in seven Lagerstroemia indica L.varieties. Through comparing with the field observation to research the effect of low temperature on chilling tolerance parameters of Lagerstroemia indica L. seedlings and the relationship between different appraisal methods and the chilling tolerance of different Lagerstroemia indica L.varieties.