Fusarium spp. in Jiangsu: Species Detection and Resistance Analysis

doi:10.11923/j.issn.2095-4050.cjas20190600089

Abstract

Abstract:

The study aims to detect the species composition of Fusarium spp. in Jiangsu and the resistance severity to traditional fungicides. The ascospores of Fusarium spp. from Liyang, Tongzhou and Yancheng in Jiangsu Province were used as materials to identify its dominant species and detect the resistance to carbendazim, tebuconazole and prochloraz. The results showed that Fusarium asiaticum was detected in all Fusarium spp. strains in Jiangsu, it was the absolute dominant species; the proportion of Fusarium graminearum was the highest (14%) in Liyang, the lowest in Tongzhou (8%), the average proportion was 11.33%, significantly lower than the proportion of F. asiaticum; the resistance frequency of Fusarium spp. to carbendazim in Jiangsu was between 26.3% and 54.5%; no Fusarium spp. strain resistant to tebuconazole and prochloraz was detected. In summary, the dominant species of Fusarium spp. in Jiangsu is F. asiaticum, and it has developed strong resistance to single carbendazim, and no resistance to tebuconazole and prochloraz.

Key words: Jiangsu, Fusarium spp., Ascospore, Dominant Specie, Resistance to Drug

CLC Number:

S432.1

Zhou Huafei, Wu Jiawen, Yang Hongfu, Chen Hongzhou, Shu Zhaolin, Rao Bin, Zhu Jianfei, Yao Kebing. Fusarium spp. in Jiangsu: Species Detection and Resistance Analysis[J]. Journal of Agriculture, 2020, 10(5): 31-35.

Figures/Tables 4

References 45

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病原菌	扩增条件	灵敏度	引物序列(5?-3?)
F. graminearum	62℃ 60 min	100	F3：GTCCAATCCACTCCATCCTC^[30]
			B3：CGGTCTTCTCGAGAGGTTCA
			FIP：GTGTGTGGGATGGTGGCACTACAAGTCAAATCACCCATGCG
			BIP：CTTTCGTCTCCCGGCACAATGGTCTTGTCCCAACGATGAGGA
			LB：CCCGCTCAGAAGAATTCTTCC
F. asiaticum	63℃ 60 min	70	F3：GAGACTGGTAATCAAGGTGATGA^[31]
			B3：CGTACAATTCCTGAACCAGATGA
			FIP：GTGATCAGGAAGAGGTTCCCCATCGACGGATATGGTTTGGACGCTT
			BIP：GCTGATCGCCATCCTGATGGGGGCTTATGAGCCAGATTGAGAA
			LB：GCCGCTAGTGATGCTTGGCTTA

病原菌	扩增条件	灵敏度	引物序列(5?-3?)
F. graminearum	62℃ 60 min	100	F3：GTCCAATCCACTCCATCCTC^[30]
			B3：CGGTCTTCTCGAGAGGTTCA
			FIP：GTGTGTGGGATGGTGGCACTACAAGTCAAATCACCCATGCG
			BIP：CTTTCGTCTCCCGGCACAATGGTCTTGTCCCAACGATGAGGA
			LB：CCCGCTCAGAAGAATTCTTCC
F. asiaticum	63℃ 60 min	70	F3：GAGACTGGTAATCAAGGTGATGA^[31]
			B3：CGTACAATTCCTGAACCAGATGA
			FIP：GTGATCAGGAAGAGGTTCCCCATCGACGGATATGGTTTGGACGCTT
			BIP：GCTGATCGCCATCCTGATGGGGGCTTATGAGCCAGATTGAGAA
			LB：GCCGCTAGTGATGCTTGGCTTA

取样地点	亚细亚镰孢占比/%	禾谷镰孢占比/%	优势种群
江苏通州	100	8	亚细亚镰孢
江苏盐城	100	12	亚细亚镰孢
江苏溧阳	100	14	亚细亚镰孢

取样地点	亚细亚镰孢占比/%	禾谷镰孢占比/%	优势种群
江苏通州	100	8	亚细亚镰孢
江苏盐城	100	12	亚细亚镰孢
江苏溧阳	100	14	亚细亚镰孢

取样地点	多菌灵5 μg/mL	多菌灵10 μg/mL
江苏通州	54.5	14.2
江苏盐城	26.3	3.3
江苏溧阳	34.8	4.8
平均值	38.5	7.4