Effects of Bacterial Fertilizer Combined with Amino Acid Water-soluble Fertilizer on Rhizosphere Soil Micro-ecological Environment and Growth of Tobacco Plants

doi:10.11923/j.issn.2095-4050.cjas2025-0012

Abstract

Abstract:

To investigate the effects of bacterial fertilizer and amino water-soluble fertilizer combined application on rhizosphere soil and growth of tobacco plants under the condition of chemical fertilizer control, a field experiment was conducted to study the effects of bacterial fertilizer combined with amino water-soluble fertilizer on tobacco plant growth, rhizosphere soil physical and chemical properties, enzyme activity, bacterial community structure and diversity under the condition of 10% nitrogen reduction, with conventional fertilization as control (A0B1). The results showed that compared with A0B1, under the condition of 10% nitrogen reduction, bacterial fertilizer combined with amino water-soluble fertilizer showed various effects. (1) The contents of organic matter, alkali-hydrolyzable nitrogen and available P and the activity of catalase and urease in rhizosphere soil increased, and the activity of nitrate reductase decreased. (2) The diversity and relative abundance of bacteria and fungi in rhizosphere soil were increased, the relative abundance of specific bacteria, fungi and dominant bacteria (Chloroflexi, Acidobacteria and Gemmatimonadetes) increased, and the relative abundance of dominant bacteria (Actinobacteria and Entorrhizomycota) decreased. (3) Redundancy analysis showed that soil pH was the key environmental factor positively correlated with the abundance of bacterial and fungal communities, and available P was negatively which correlated with the relative abundance of bacterial communities. (4) This combined application method promoted the growth and development of tobacco and leaf opening. In general, under the condition of 10% nitrogen reduction, the application of bacterial fertilizer combined with amino soluble fertilizer can improve the rhizosphere soil nutrient level, improve the rhizosphere micro-ecological environment, and promote the growth and development of tobacco plants, the overall effect of bacterial fertilizer+75.0 g/hm² amino water-soluble fertilizer treatment(A1B3) is better.

Key words: high-throughput sequencing, flue-cured tobacco, amino water-soluble fertilizer, biodiversity, rhizosphere soil

YANG Qigang, HU Ronghua, WANG Zheng, ZHAO Dongjie, AO Jincheng. Effects of Bacterial Fertilizer Combined with Amino Acid Water-soluble Fertilizer on Rhizosphere Soil Micro-ecological Environment and Growth of Tobacco Plants[J]. Journal of Agriculture, 2025, 15(11): 49-57.

Figures/Tables 7

References 29

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处理	pH	有机碳/(g/kg)	有机质/(g/kg)	碱解氮/(mg/kg)	速效磷/(mg/kg)	速效钾/(mg/kg)
A0B1	6.6±0.2 ab	18.3±0.6 C	31.5±1.1 C	65.9±1.8 ab	41.7±8.8 c	343.7±8.0 a
A0B2	6.7±0.1 ab	21.2±1.7 B	36.5±3.0 B	50.8±13.6 b	48.2±4.6 c	314.5±26.6 b
A0B3	6.8±0.0 a	21.2±1.2 B	36.6±2.0 B	58.9±14.1 ab	70.0±3.6 b	302.9±9.9 b
A1B1	6.8±0.0 a	22.0±1.2 B	38.0±2.0 B	75.3±18.8 ab	71.6±5.5 b	290.0±3.4 b
A1B2	6.5±0.2 b	24.8±1.3 A	42.7±2.3 A	72.9±4.8 ab	84.4±4.7 a	318.6±15.7 ab
A1B3	6.8±0.1ab	26.0±0.1 A	44.8±0.2 A	78.8±6.3 a	88.2±3.2 a	302.5±6.3 b

处理	pH	有机碳/(g/kg)	有机质/(g/kg)	碱解氮/(mg/kg)	速效磷/(mg/kg)	速效钾/(mg/kg)
A0B1	6.6±0.2 ab	18.3±0.6 C	31.5±1.1 C	65.9±1.8 ab	41.7±8.8 c	343.7±8.0 a
A0B2	6.7±0.1 ab	21.2±1.7 B	36.5±3.0 B	50.8±13.6 b	48.2±4.6 c	314.5±26.6 b
A0B3	6.8±0.0 a	21.2±1.2 B	36.6±2.0 B	58.9±14.1 ab	70.0±3.6 b	302.9±9.9 b
A1B1	6.8±0.0 a	22.0±1.2 B	38.0±2.0 B	75.3±18.8 ab	71.6±5.5 b	290.0±3.4 b
A1B2	6.5±0.2 b	24.8±1.3 A	42.7±2.3 A	72.9±4.8 ab	84.4±4.7 a	318.6±15.7 ab
A1B3	6.8±0.1ab	26.0±0.1 A	44.8±0.2 A	78.8±6.3 a	88.2±3.2 a	302.5±6.3 b

分类	处理	Sobs指数	Chao指数	Shannon指数	PD指数
细菌	A0B1	1575.7±78.1 B	1686.3±66.6 a	8.9±0.3 b	100.1±4.2 b
	A0B2	1684.0±5.6 A	1739.1±5.8 a	9.3±0.1 a	105.6±1.0 a
	A0B3	1661.0±22.6 A	1735.6±24.9 a	9.3±0.1 a	105.2±0.9 a
	A1B1	1671.0±16.1 A	1743.3±29.2 a	9.3±0.0 a	105.1±1.7 a
	A1B2	1652.3±20.7 A	1743.2±15.0 a	9.1±0.1 ab	103.4±1.8 ab
	A1B3	1671.0±16.3 A	1755.8±14.8 a	9.2±0.0 a	105.2±1.5 a
真菌	A0B1	259.7±46.9 a	292.9±31.9 a	3.1±1.6 a	40.3±6.5 a
	A0B2	294.7±22.4 a	319.9±12.6 a	3.3±1.1 a	44.8±3.1 a
	A0B3	274.0±43.0 a	292.9±37.5 a	3.6±0.8 a	42.7±4.7 a
	A1B1	321.7±10.0 a	338.0±8.8 a	5.3±0.5 a	47.6±2.2 a
	A1B2	285.3±26.7 a	305.7±18.7 a	3.8±1.2 a	43.7±3.6 a
	A1B3	298.0±29.9 a	318.0±42.0 a	3.7±0.4 a	42.3±3.3 a

分类	处理	Sobs指数	Chao指数	Shannon指数	PD指数
细菌	A0B1	1575.7±78.1 B	1686.3±66.6 a	8.9±0.3 b	100.1±4.2 b
	A0B2	1684.0±5.6 A	1739.1±5.8 a	9.3±0.1 a	105.6±1.0 a
	A0B3	1661.0±22.6 A	1735.6±24.9 a	9.3±0.1 a	105.2±0.9 a
	A1B1	1671.0±16.1 A	1743.3±29.2 a	9.3±0.0 a	105.1±1.7 a
	A1B2	1652.3±20.7 A	1743.2±15.0 a	9.1±0.1 ab	103.4±1.8 ab
	A1B3	1671.0±16.3 A	1755.8±14.8 a	9.2±0.0 a	105.2±1.5 a
真菌	A0B1	259.7±46.9 a	292.9±31.9 a	3.1±1.6 a	40.3±6.5 a
	A0B2	294.7±22.4 a	319.9±12.6 a	3.3±1.1 a	44.8±3.1 a
	A0B3	274.0±43.0 a	292.9±37.5 a	3.6±0.8 a	42.7±4.7 a
	A1B1	321.7±10.0 a	338.0±8.8 a	5.3±0.5 a	47.6±2.2 a
	A1B2	285.3±26.7 a	305.7±18.7 a	3.8±1.2 a	43.7±3.6 a
	A1B3	298.0±29.9 a	318.0±42.0 a	3.7±0.4 a	42.3±3.3 a

处理	株高/cm	叶数/片	茎围/cm	最大腰叶		叶面积/cm²	顶叶		叶面积/cm²
处理	株高/cm	叶数/片	茎围/cm	叶长/cm	叶宽/cm	叶面积/cm²	叶长/cm	叶宽/cm	叶面积/cm²
A0B1	111.8±5.7b	18.2 ±0.8 b	11.1±0.7 a	67.0±1.9 b	35.6±4.3 a	1535.5±196.0 a	29.8 ±3.5 b	9.6±2.0 a	187.0±60.8 b
A0B2	115.6±3.4 b	18.8±1.1 b	10.4± 0.8 a	68.8±5.8 ab	35.0±3.9 a	1559.2±277.2 a	30.8±2.9 ab	9.9±1.7 a	196.3±50.1 b
A0B3	114.4 ±3.8 b	18.4±0.8 b	10.4±0.9 a	68.0±3.3 ab	36.0±5.3 a	1575.5 ±248.0 a	30.4±3.1 b	11.4 ±1.6 a	222.1±38.5 ab
A1B1	121.2±2.0 a	19.4±1.1 ab	11.0±0.3 a	71.4±4.7 ab	35.4±4.0 a	1627.0±215.0 a	30.0±2.3 b	11.4 ±0.8 a	220.1±22.9 ab
A1B2	121.6±2.5 a	20.2±0.4 ab	10.0±0.7 a	73.2±3.8 a	36.0±2.9 a	1696.5±194.1 a	31.6 ±4.4 ab	10.4±2.1 a	212.5±58.9 ab
A1B3	124.2±3.2 a	21.0±1.1 a	10.9 ±0.6a	73.2±5.5 a	37.0 ±4.9 a	1743.6 ±283.3 a	34.2±2.0 a	11.8 ±0.9 a	260.4±26.3 a