To explore the coupling effect of water and fertilizer integration technology on mulberry orchards in rain-fed areas of hot and dry valleys, fresh mulberries in Yanbian County were studied. The study area was divided into four types: slope rain-fed area (DN), channel rain-fed area (WN), slope fertilization area (DF) and channel fertilization area (WF). The differences in fruit length, fruit diameter, volume, weight, density, fruit shape index and sugar content of mulberries in four types of regions were compared. The results showed that: (1) fertilization could affect the phenological characteristics of mulberry fruits and accelerate mulberry maturation; (2) fertilization, micro-topography, integrated fertilization and micro-topography had significant effects on fruit length, fruit diameter, volume and fruit shape index of fresh mulberry; (3) maturity could significantly affect fruit length, diameter and volume of fresh mulberry, but had no significant effect on fruit shape index of fresh mulberry; (4) in DF and WF, the higher the maturity, the higher the density and quality of mulberry; in DN, the density and quality of over-mature mulberry were significantly higher than those of just-ripe and full-ripe mulberry; however, in WN, the density and quality of mature mulberry were significantly higher than those of over-ripe and just-ripe mulberry; (5) the highest average sugar content was over-mature mulberry in WF, with an average sugar content of 18.01%, while the lowest was just mature mulberry in WN, with an average sugar content of 11.76%; (6) the differences of commercial characters of mulberry in DN and WN were mainly the differences of shape and sugar content; mulberry in WN had lower sugar content and larger size than that in DN; (7) there was no significant difference in commercial traits between mulberries in WF and DF. These results reveal the internal mechanism of water and nutrient supply regulating the commodity characters of mulberry, that is, water supply can promote the growth and weight of fresh mulberry, and fertilization can make fresh mulberry sweeter.