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农学学报 ›› 2015, Vol. 5 ›› Issue (12): 94-98.doi: 10.11923/j.issn.2095-4050.cjas15050009

所属专题: 生物技术

• 畜牧 兽医 水产 • 上一篇    下一篇

6种紫花苜蓿越冬前后几个抗寒生理指标变化研究

申晓慧,姜成,冯鹏,李如来,李建东,郑海燕   

  1. 黑龙江省农业科学院 佳木斯分院,佳木斯大学生命科学学院,黑龙江省农业科学院 佳木斯分院,黑龙江省农业科学院 佳木斯分院,沈阳农业大学农学院,黑龙江省农业科学院 佳木斯分院
  • 收稿日期:2015-05-08 修回日期:2015-07-13 接受日期:2015-07-24 出版日期:2016-01-07 发布日期:2016-01-07
  • 通讯作者: 冯鹏 E-mail:15904540498
  • 基金资助:
    黑龙江省博士后基金“紫花苜蓿抗寒关键技术及机制研究”(LBH-Z12239)。

Research on Changing of Several Cold Resistance Physiological Indexes of 6 kinds of Alfalfa before and after Overwintering

  • Received:2015-05-08 Revised:2015-07-13 Accepted:2015-07-24 Online:2016-01-07 Published:2016-01-07

摘要: 研究北方高寒地区对紫花苜蓿越冬生理生化特性的影响,以期为当地紫花苜蓿推广应用提供理论依据。以越冬前后苜蓿根系为试验材料,于2013 年10 月15 日、10 月30 日、11 月15 日、2014 年3 月30日、4 月15 日、4 月30 日挖根采样,-80℃冰箱保存,并于2014 年春进行越冬率调查。通过测定6 种紫花苜蓿在高寒地区越冬前后可溶性糖、全氮、可溶性蛋白和游离脯氨酸含量,对不同紫花苜蓿品种抗寒生理特性进行了比较研究。结果表明:‘公农1 号’和‘草原3 号’的可溶性糖、可溶性蛋白及游离脯氨酸含量 在越冬最冷月(11 月)含量高于其他品种,其次是‘龙牧806’和‘Wega7F’,均具有较高的抗寒能力,而‘敖汉’和‘WL319 HQ’的各项指标略低,抗寒能力较弱。供试的6 种紫花苜蓿在佳木斯地区整个越冬期间其抵抗严寒能力大小为:‘公农1号’>‘草原3号’>‘龙牧806’>‘Wega7F’>‘敖汉’>‘WL319 HQ’。

关键词: 水稻, 水稻, 种植密度, 农艺性状, 产量

Abstract: This research was conducted to study the effect of the north cold area on the physiological and biochemical characters of alfalfa during overwntering in order to provide theoretical basis for wide application of alfalfa. The roots of alfalfa before and after winter were used as materials. The roots were dug on 15th October, 30th October and 15th November in 2013 and 30th March, 15th April and 30th April in 2014, and then preserved in -80℃ refrigerator. Overwintering rate was surveyed in the spring of 2014. Based on analysis of main quality indexes, including soluble sugar content, soluble protein content, total nitrogen and free praline content, effects of physicochemical indexes of roots of different kinds of alfalfa were studied. The result showed that the content of soluble sugar and soluble protein, free praline of roots of‘Gongnong No.1’and‘Caoyuan No.3’were the highest among all the alfalfa varieties in the coldest month (November), followed by‘Longmu 806’and ‘Wega7F’, which had higher cold resistance ability. However, every index of roots of‘Aohan’and‘WL319 HQ’were lower than that of other varieties, their ability of cold resistance was relatively poor. Therefore, the ability of cold resistance of the six alfalfa varieties was in an order as:‘Gongnong No.1’>‘Caoyuan No.3’> ‘Longmu 806’>‘Wega7F ‘Aohan’>‘WL319 HQ’.

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