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农学学报 ›› 2022, Vol. 12 ›› Issue (8): 73-80.doi: 10.11923/j.issn.2095-4050.cjas2021-0127

所属专题: 生物技术 植物保护

• 农业工程/农业机械/生物技术/食品科学 • 上一篇    下一篇

市售农残速测试剂盒对5种有机磷农药的敏感性评价

罗俊霞1(), 赵建波2(), 赵倩倩3(), 张刚4, 王峰2, 刘青5, 张洁2, 潘玲1, 赵利敏2, 马蕾2   

  1. 1郑州市农产品质量检测流通中心,郑州 450006
    2郑州市农业技术推广中心,郑州 450002
    3焦作市农产品质量安全检测中心,河南焦作 454002
    4河南恒晟检测技术有限公司,河南新郑 451100
    5郑州市农业综合行政执法支队,郑州 450002
  • 收稿日期:2021-07-08 修回日期:2021-09-13 出版日期:2022-08-20 发布日期:2022-09-22
  • 通讯作者: 赵建波,赵倩倩 E-mail:71819@163.com;13939021082@139.com;714871827@qq.com
  • 作者简介:罗俊霞,女,1972年出生,本科,高级农艺师,从事农产品质量安全检测与研究工作。通信地址:450006 郑州市淮河西路63号 郑州市农产品质量检测流通中心,E-mail: 71819@163.com
  • 基金资助:
    河南省郑州市农产品质量检测中心建设项目(豫发改农经(2011)1778号);郑州市检测检验体系建设项目(郑农检(2012)12号);郑州市农产品质量安全追溯体系建设项目(郑发改农经[2013]126号)

Sensitivity Evaluation of the Test Kits for the Determination of the Residual Rate of Five Organophosphorus Pesticides in Agricultural Products

LUO Junxia1(), ZHAO Jianbo2(), ZHAO Qianqian3(), ZHANG Gang4, WANG Feng2, LIU Qing5, ZHANG Jie2, PAN Ling1, ZHAO Limin2, MA Lei2   

  1. 1Zhengzhou Agricultural Product Quality Testing and Distribution Center, Zhengzhou 450006, Henan, China
    2 2Zhengzhou Agricultural Technology Extension Centre, Zhengzhou 450002, Henan, China
    3Jiaozuo Agricultural Products Quality and Safety Testing Center, Jiaozuo 454002, Henan, China
    4Henan Hengsheng Testing Technology Co., Ltd., Xinzheng 451100, Henan, China
    5Zhengzhou Agricultural Comprehensive Administrative Law Enforcement Detachment, Zhengzhou 450002, Henan, China
  • Received:2021-07-08 Revised:2021-09-13 Online:2022-08-20 Published:2022-09-22
  • Contact: ZHAO Jianbo,ZHAO Qianqian E-mail:71819@163.com;13939021082@139.com;714871827@qq.com

摘要:

为了对商品化农残速测试剂盒进行评价,全面了解市售农残速测试剂盒的质量,本研究采用市售的6个品牌农残速测试剂盒,在2种情况下(提取液取用2.5 mL和3 mL)对甲拌磷、蝇毒磷、二嗪磷、对硫磷、久效磷进行敏感性测定,根据5种农药在5个浓度水平下的测定结果,绘制6个品牌的试剂盒的抑制率对农药浓度的效应曲线并用抑制率和农药浓度之对数进行线性拟合、计算出相关系数、IC50IC10,根据IC10对各种试剂盒对每种农药的敏感性进行比较并以此判定试剂盒的优劣。结果表明同一种试剂盒对不同的农药、不同的试剂盒对同一种农药的敏感性均不同;抑制率和农药浓度对数相关性较好的试剂盒其相关系数R2分别为0.4835~1(提取液取用2.5 mL)和0.6602~1(提取液取用3 mL),IC10分别为2.01×10-4~3.78 mg/kg(提取液取用2.5 mL)和3.92×10-3~3.36 mg/kg(提取液取用3 mL);6种试剂盒对甲拌磷均不敏感,除了A试剂盒外其他试剂盒在5个浓度水平下抑制率均为0。根据所测试的5种农药,将试剂盒分为3个等次,D试剂盒最优,其次是C和E,第三是A、B和F。建议国标和行标修改50%或者70%的判定阈值。

关键词: 试剂盒, 农药, 半抑制浓度,检出限, 敏感性, 线性范围

Abstract:

To evaluate the commercial test kits for pesticide residue rate and understand their quality, the sensitivity of six brands of pesticide residue rapid test kits sold in market to phorate, phoxim, diazinon, parathion and moncrotophos were determined in two cases (2.5 mL and 3 mL of extract). The effect curves of inhibition rate on pesticide concentration of the kits were plotted according to the determination results of the five pesticides at five concentration levels, the logarithm of inhibition rate and pesticide concentration were used for linear fitting, and the correlation coefficient, IC50 and IC10 were calculated, the sensitivity of various kits to each pesticide were compared according to IC10. Then the advantages and disadvantages of the kits were determined. The sensitivity of the same kit to different pesticides and different kits to the same pesticide were different. The kits with better logarithmic correlation between inhibition rate and pesticide concentration had correlation coefficient R2 of 0.4835-1 (2.5 mL of extract) and 0.6602-1 (3 mL of extract); and IC10 was 2.01×10-4-3.78 mg/kg (2.5 mL of extract) and 3.92×10-3-3.36 mg/kg (3 mL of extract) respectively. The six kits were not sensitive to phorate. Except kit A, the inhibition rates of the other kits at five concentration levels were 0. According to the five pesticides tested, the kits are divided into three grades. D kit is the best, followed by C and E, and the third is A, B and F. It is suggested to modify the judgment threshold of 50% or 70% of the national standard and industrial standard.

Key words: test kit, pesticide, semi-inhibition concentration, detection limit, sensitivity, linear range

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