瑞安陶山甘蔗国家地理标志产地土壤结构与微生物群落分析

doi:10.11923/j.issn.2095-4050.cjas2024-0222

农学学报 ›› 2026, Vol. 16 ›› Issue (3): 73-79.doi: 10.11923/j.issn.2095-4050.cjas2024-0222

• 土壤肥料资源环境生态 • 上一篇下一篇

瑞安陶山甘蔗国家地理标志产地土壤结构与微生物群落分析

叶挺云¹(), 麻理亚¹, 卢升高²()

¹ 瑞安市农业农村发展中心，浙江瑞安 325200
² 浙江大学环境与资源学院，杭州 310058

收稿日期:2024-12-12 修回日期:2025-05-14 出版日期:2026-03-20 发布日期:2026-03-19
通讯作者:
卢升高。男，1962年出生，浙江东阳人，教授，博士，主要从事土壤结构与土壤健康研究。通信地址：310058 浙江省杭州市西湖区余杭塘路866号，Tel：0571-88982061，E-mail：lusg@zju.edu.cn。
作者简介:
叶挺云，男，1975年出生，浙江瑞安人，高级农艺师，本科，主要从事土壤肥料和病虫害防治等方面的研究推广。通信地址：325200 浙江省瑞安市东山街道天瑞路，Tel：0577-65812199，E-mail：715040949@qq.com。
基金资助:
国家自然科学基金项目“典型土壤团聚体的多尺度三维孔隙结构和有机碳物理保护机制”(41977005)

Analysis of Soil Structure and Microbial Community of Sugarcane National Geographical Indication Production Area in Taoshan of Ruian

YE Tingyun¹(), MA Liya¹, LU Shenggao²()

¹ Ruian Agricultural and Rural Development Center, Ruian, Zhejiang 325200
² College of Environmental and Resources, Zhejiang University, Hangzhou 310058

Received:2024-12-12 Revised:2025-05-14 Online:2026-03-20 Published:2026-03-19

摘要/Abstract

摘要：

以瑞安“陶山甘蔗”国家地理标志农产品产地为研究区，分析长期甘蔗种植土壤的土壤结构和微生物群落特征。项目利用湿筛法测定土壤水稳性团聚体组成和稳定性，Miseq高通量测序测定土壤微生物群落多样性，分析土壤结构与微生物丰度和多样性的关系。根据土壤>0.25 mm水稳性团聚体含量、团聚体平均重量直径(MWD)和平均几何直径(GMD)，将甘蔗土壤结构稳定性分为高(HS)、中(MS)和低(LS) 3个等级，HS土壤的pH和有机质含量显著高于MS和LS，而不同结构土壤的速效磷、碱解氮和速效钾无显著差异。HS土壤的微生物碳(MBC)和氮(MBN)含量显著增高。微生物群落结构分析表明：土壤结构显著影响微生物的OTU数量、α多样性指数和群落组成，HS土壤的稳定性团聚体和较高含量的有机质为微生物提供适宜的微生境。土壤结构也影响微生物优势菌属的丰度，冗余分析表明土壤结构通过改变土壤理化性质影响土壤微生物优势属的丰度，良好的土壤结构能够为微生物提供更加适宜的生境。研究结果初步揭示了长期甘蔗种植土壤的结构与微生物群落特征及其相互关系，为研究调节甘蔗土壤结构，优化微生物多样性及功能，增强土壤健康功能提供了借鉴，对长期甘蔗种植土壤的管理和持续生产提供了科学依据。

关键词: 土壤结构, 土壤团聚体稳定性, 微生物群落结构, 甘蔗, 国家地理标志农产品产地

Abstract:

The soil structure and microbial community characteristics of long-term sugarcane cultivation in the national geographical indication agricultural product origin of ‘Taoshan sugarcane’ in Ruian, Zhejiang Province were analyzed. This project used wet-screening method to determine the composition and stability of soil water-stable aggregates, and Miseq high-throughput sequencing was adopted to measure soil microbial community, and then the relationship between soil structure and microbial abundance and diversity was explored. Based on the content of >0.25 mm water-stable aggregate, mean weight diameter (MWD) and geometric mean diameter (GMD) of aggregate, the structural stability of sugarcane soil was divided into three levels: high (HS), medium (MS) and low (LS). The pH and organic matter content of HS soil were significantly higher than those of MS and LS soils, while there was no significant difference in available phosphorus, hydrolyzed nitrogen and available potassium among soils with different structures. The HS soil had significantly higher microbial carbon (MBC) and nitrogen (MBN) content relative to MS and LS soils. The analysis of microbial community structure showed that soil structure significantly affected the OTU quantity, alpha diversity index, and community composition of microorganisms. The HS soil with higher contents of stable aggregate and organic matter provided a suitable microbial habitat for microorganisms. Soil structure also affected the abundance of dominant microbial genera. Redundancy analysis (RDA) showed that soil structure affected the abundance of dominant microbial genera by altering soil physicochemical properties. A good soil structure could provide a more suitable habitat for microorganisms. The research results had preliminarily revealed the soil structure and microbial community characteristics of long-term sugarcane plantation soil, as well as their interrelationships. This provided a reference for studying the regulation of sugarcane soil structure, optimization of microbial diversity and function in order to enhance soil health function. This result also provided scientific basis for the management and sustainable utilization of long-term sugarcane plantation soil.

Key words: soil structure, soil aggregate stability, microbial community structure, sugarcane, national geographical indication production area

叶挺云, 麻理亚, 卢升高. 瑞安陶山甘蔗国家地理标志产地土壤结构与微生物群落分析[J]. 农学学报, 2026, 16(3): 73-79.

YE Tingyun, MA Liya, LU Shenggao. Analysis of Soil Structure and Microbial Community of Sugarcane National Geographical Indication Production Area in Taoshan of Ruian[J]. Journal of Agriculture, 2026, 16(3): 73-79.

图/表 7

参考文献 18

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指标	LS	MS	HS
WSA/%	65.92±10.76 c	86.06±5.24 b	93.61EMBED Word.Document.8 EMBED Excel.Sheet.8 EMBED Excel.Sheet.8 ±5.46 a
MWD/mm	1.36±0.36 c	2.57±0.45 b	3.84±0.49 a
GMD/mm	0.68±0.29 c	1.5±0.42 b	2.85±0.75 a

指标	LS	MS	HS
WSA/%	65.92±10.76 c	86.06±5.24 b	93.61EMBED Word.Document.8 EMBED Excel.Sheet.8 EMBED Excel.Sheet.8 ±5.46 a
MWD/mm	1.36±0.36 c	2.57±0.45 b	3.84±0.49 a
GMD/mm	0.68±0.29 c	1.5±0.42 b	2.85±0.75 a

指标	LS	MS	HS
pH	4.18±0.56 b	4.60±0.61 a	4.73±0.48 a
有机质/(g/kg)	17.33±5.7 b	18.47±7.98 b	22.26±6.09 a
速效磷/(mg/kg)	4.63±1.83 a	5.38±2.83 a	5.26±2 a
碱解氮/(mg/kg)	216.36±52 a	181.27±33.92 a	278.13±45.52 a
速效钾/(mg/kg)	91.44±55.53 a	117.69±79.92 a	84.43±49.25 a
砂粒/%	40.11±15.41 a	37.17±10.17 a	30.38±13.74 b
粉粒/%	40.6±11.76 a	37.56±9.72 a	47.4±13.56 a
黏粒/%	19.29±3.65 b	25.27±10.07 a	22.22±9.02 ab
土壤含水率/%	22.72±1.32 b	26.43±4.74 a	29.15±7.9 a
田间持水量/%	31.74±5.42 a	33.69±4.01 a	32.83±4.96 a
土壤容重/(g/cm³)	1.33±0.16 a	1.29±0.06 a	1.31±0.18 a

指标	LS	MS	HS
pH	4.18±0.56 b	4.60±0.61 a	4.73±0.48 a
有机质/(g/kg)	17.33±5.7 b	18.47±7.98 b	22.26±6.09 a
速效磷/(mg/kg)	4.63±1.83 a	5.38±2.83 a	5.26±2 a
碱解氮/(mg/kg)	216.36±52 a	181.27±33.92 a	278.13±45.52 a
速效钾/(mg/kg)	91.44±55.53 a	117.69±79.92 a	84.43±49.25 a
砂粒/%	40.11±15.41 a	37.17±10.17 a	30.38±13.74 b
粉粒/%	40.6±11.76 a	37.56±9.72 a	47.4±13.56 a
黏粒/%	19.29±3.65 b	25.27±10.07 a	22.22±9.02 ab
土壤含水率/%	22.72±1.32 b	26.43±4.74 a	29.15±7.9 a
田间持水量/%	31.74±5.42 a	33.69±4.01 a	32.83±4.96 a
土壤容重/(g/cm³)	1.33±0.16 a	1.29±0.06 a	1.31±0.18 a

		LS	MS	HS
真菌	Chao1	880.48±126 a	824.20±206 ab	710.63±200 b
	Shannon	4.74±0.6 b	5.09±0.7 a	5.01±0.63 a
	Simpson	0.11±0.04 a	0.09±0.04 b	0.08±0.04 b
细菌	Chao1	5462.00±946.86 a	5135.86±126.40 b	5401.94±797.12 a
	Shannon	8.83±0.92 b	9.04±0.79 a	9.30±0.6 a
	Simpson	0.019±0.006 a	0.012±0.004 b	0.008±0.004 b

瑞安陶山甘蔗国家地理标志产地土壤结构与微生物群落分析

Analysis of Soil Structure and Microbial Community of Sugarcane National Geographical Indication Production Area in Taoshan of Ruian

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