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农学学报

农学学报 ›› 2021, Vol. 11 ›› Issue (6): 25-28.doi: 10.11923/j.issn.2095-4050.cjas20200300045

所属专题： 生物技术； 农业气象

• 农艺科学/生理生化 • 上一篇    下一篇

PEG模拟干旱胁迫对聚合草叶片生理特性的影响

刘永华(), 李鲜花, 崔凡, 刘翠英()   

  1. 榆林学院生命科学学院,陕西榆林 719000
  • 收稿日期:2020-03-04 修回日期:2020-04-22 出版日期:2021-06-20 发布日期:2021-06-28
  • 通讯作者: 刘翠英 E-mail:liuyonghuaa@126.com;zzj0405@163.com
  • 作者简介:刘永华,男,1978年出生,山西阳泉人,副教授,博士,主要从事植物保护与育种研究。通信地址：719000 榆林学院生命科学院,Tel：0912-3891342,E-mail： liuyonghuaa@126.com。
  • 基金资助:
    陕西省生态恢复与重建实验室开放项目“榆神府煤矿废弃地生态修复植物种的筛选及优化配置研究”(KFXM-201803);榆林市科技局科技攻关项目“榆林市乡土观赏植物调查及园林绿化应用研究”(2018-cxy-2)

PEG Simulating Drought Stress: Effect on Physiological Characteristics of Symphytum officinale Leaves

Liu Yonghua(), Li Xianhua, Cui Fan, Liu Cuiying()   

  1. Life and Science College, Yulin University, Yulin 719000, Shaanxi, China
  • Received:2020-03-04 Revised:2020-04-22 Online:2021-06-20 Published:2021-06-28
  • Contact: Liu Cuiying E-mail:liuyonghuaa@126.com;zzj0405@163.com

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摘要：

为研究PEG模拟干旱胁迫环境下聚合草叶片的生理变化,设置不同浓度的PEG溶液(0%、5%、10%、15%、20%、25%)进行处理,对其可溶性糖含量、可溶性蛋白含量、游离脯氨酸含量、过氧化物酶活性、超氧化物歧化酶活性进行测定。结果表明,随着PEG浓度(0%~25%)的增加,可溶性糖含量、可溶性蛋白质含量、POD活性、SOD活性均呈现先上升后下降的趋势。当PEG浓度上升到10%时,POD和SOD活性达到最高值,分别为270.3 U/(g·min)、1167.7 U/g;可溶性糖和可溶性蛋白质含量达到最高值,分别为1.9 μg/g、236.2 μg/g。游离脯氨酸含量随着PEG浓度的升高表现为先下降后上升的趋势,当浓度为10%时,脯氨酸含量到达最低值20.3 μg/g。研究结果表明聚合草具有一定的抗旱性,在轻度干旱环境下通过调节能力可以缓解自身受到的伤害。

关键词: 聚合草, PEG, 干旱胁迫, 生理特性, 抗旱性

Abstract:

To study the physiological changes of Symphytum officinale leaves under PEG simulating drought stress, different concentrations of PEG (0%, 5%, 10%, 15% 20%, 25%) were set up. The soluble sugar content, soluble protein content, free proline content, peroxidase activity and superoxide dismutase activity were measured. The results showed that with the increase of PEG concentrations (0%~25%), the soluble sugar content, the soluble protein content, POD activity and SOD activity increased first and then decreased. When the PEG concentration increased to 10%, POD activity and SOD activity reached the highest value, 270.3 U/(g·min) and 1167.7 U/g, respectively; the soluble sugar content and the soluble protein content was up to the maximum, 1.9 μg/g, 236.2 μg/g, respectively. The free proline content decreased first and then increased with the increase of PEG concentrations. When the concentration was 10%, the proline content reached the lowest value of 20.3 μg/g. It indicates that Symphytum officinale has a certain drought resistance, and it could alleviate the damage suffered through own adjustment ability in mild drought environment.

Key words: Symphytum officinale, PEG, Drought Stress, Physiological Characteristics, Drought Resistance

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