Callus Induction and Plant Regeneration from Young Leaf Segments of Sorghum bicolor

doi:10.11923/j.issn.2095-4050.cjas18020007

Abstract

Abstract: To establish a sorghum tissue culture system with young leaf segments as explants, eight sorghum restorer lines commonly used in breeding are selected, and young leaf segments are used as explants for callus induction and plant regeneration. The effect of 2,4-D concentration on primary callus induction is studied. The callus induction and differentiation frequency are recorded, and the callus morphology is also observed. The results showed that 2,4-D was the key factor inducing callus of young leaves. No callus could be produced in medium without 2,4-D. The suitable 2,4-D concentration was 2 mg/L. Genotype had a significant effect on callus quality and morphology, and the callus induction and differentiation rate of these eight genotypes were very different. Three genotypes,‘Sanchisan’,‘R111’and‘8643 bian’, were selected after screening for their performance of higher callus induction rate, excellent morphology and higher differentiation rate.

CLC Number:

S514

References

[1] Masteller V J,Holden D J.The growth and organ formation from Callus tissue of sorghum.Plant Physiology,1970,45:362-364.
[2] Gamborg O L,Shyluk J P,Brar D S,et al.Morphogenesis and plant regeneration from callus of immature embryos of sorghum.Plant Science Letters,1977,10(1):67-74.
[3] Bhaskaran S,Smith R H.Control of Morphogenesis in Sorghum by 2,4-Dichlorophenoxyacetic Acid and Cytokinins.Annals of Botany,1989,64(2):217-224.
[4] Kishore N S, Visarada K B R S, Lakshmi Y A, et al. In vitro culture methods in sorghum with shoot tip as the explant material. Plant Cell Reports, 2006, 25: 174–182.
[5] Jogeswar G, Ranadheer D, Anjaiah V, et al. High frequency somatic embryogenesis and regeneration in different genotypes of Sorghum bicolor (L.) Moench from immature inflorescence explants. In Vitro Cellular and Developmental Biology-Plant, 2007, 43: 159–166.
[6] Kumaravadivel N, Rangasamy S R S . Plant regeneration from sorghum anther cultures and field evaluation of progeny. Plant Cell Reports, 1994, 13: 286-290.
[7] 郭建华．高粱幼胚小盾片愈伤组织的诱导及其再生植株性状的变异分析．辽宁农业科学,1989(3):7-14．
[8] 白志良,王良群,郑丽萍,等.高粱不同外植体离体培养.华北农学报,1995,10(1)：60-63.
[9] Wernicke W,Brettell R I S.Morphogenesis from cultured leaf tissue of Sorghum bicolor-culture initiation.Protoplasma,1982,111:19-27.
[10] Wernicke W,Potrykus I,Thomas E.Morphogenesis from cultured leaf tissue of Sorghum bicolor-The morphogenetic pathways.Protoplasma,1982,111:53-62.
[11] 韩福光,张颖.高粱不同外植体愈伤组织诱导的研究.辽宁农业科学,1993,1:45-48.
[12] Ahmadabadi M,Ruf S,Bock R．A leaf-based regeneration and transformation system for maize(Zea mays L.).Transgenic Research,2007,16(4):437-448.
[13] 王梓全,姜丽丽,曹士亮,等.玉米叶基部愈伤组织诱导与植株再生.作物杂志,2012,(6)：65-68.
[14] 季丽丽,关淑艳,颜雪芬,等.玉米自交系幼胚愈伤组织诱导的条件优化.作物杂志,2011,(2)：60-64.
[15] 黄璐,卫志明.不同基因型玉米的再生能力和胚性与非胚性愈伤组织DNA的差异.植物生理学报,1999,25(4):332-338.
[16] 林国梁,李佳春,王兴珍,等.2,4-D和干燥处理对小麦成熟胚愈伤组织诱导与分化的影响.麦类作物学报,2012,32(5):858-862.
[17] 王良群,白志良,王呈祥,等.高粱不同外植体再生苗分化培养的研究,杂粮作物,2004,24(5):262-263.
[18] 陈靖,董浩,马海珍,等.玉米幼胚和源于幼苗的幼嫩叶段愈伤诱导及其植株再生的比较.中国农业科学,2011,44(17):3676-3682.
[19] 李玉静,陈彦龙,王玲玲,等.2,4-D和6-BA对水稻愈伤组织培养力的影响.河北师范大学学报(自然科学版),2005,29(4)：395-399.
[20] 刘燕蓉,张万军.不同生态型的11个柳枝稷品种组织培养反应评价.草地学报,2014,22(3)：579-585.
[21] 刘宣雨,王青云,刘树君,等.高粱遗传转化研究进展.植物学报,2011,46(2):216-223.
[22] 朱莉,郎志宏,李桂英,等.高粱遗传转化研究进展.生物技术通报,2011,(1)：1-8.