Identification of Codonopsis pilosula Crown Rot Disease and Its Sensitivity to Fungicides

doi:10.11923/j.issn.2095-4050.cjas2020-0052

Abstract

Abstract:

To clarify the pathogen, pathogenicity and dominant pathogen species of Codonopsis pilosula crown rot disease and their sensitivity to different fungicides, the pathogen was isolated by tissue isolation method and identified according to its culture traits, morphology and molecular biological characteristics. Meanwhile, the sensitivity of different fungicides was determined by mycelium growth inhibition method. The results showed that the disease pathogens were identified as Fusarium oxysporum, F. sonali and F. equiseti. The dominant species was F. oxysporum. Pathogenicity testing showed that the three species could cause C. pilosula crown rot disease, F. oxysporum had the strongest pathogenicity, and its disease rate and disease index had a significant difference from those of the other two. Laboratory toxicity test of different fungicides to the mycelia growth of the three species showed that difenoconazole fungicide had the strongest inhibition activity, and the three Fusarium species had the highest sensitivity to pyraclostrobin fungicide.

Key words: Codonopsis pilosula, Crown Rot Disease, Fusarium spp., Pathogen Identification, Fungicides, Sensitivity Testing

CLC Number:

S436.429

Qi Yonghong, Li Minquan, Cao Sufang, Jiang Jingjing, Li Xueping, Li Qingqing, Xu Meirong, Li Jiping. Identification of Codonopsis pilosula Crown Rot Disease and Its Sensitivity to Fungicides[J]. Journal of Agriculture, 2021, 11(2): 74-78.

Figures/Tables 4

References 25

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镰孢菌	接种数	发病率/%	病情指数
尖孢镰孢菌	25	92.0	45.2a
茄病镰孢菌	25	88.0	21.2b
木贼镰孢菌	25	80.0	17.5b
CK	25	0.0	0.0c

镰孢菌	接种数	发病率/%	病情指数
尖孢镰孢菌	25	92.0	45.2a
茄病镰孢菌	25	88.0	21.2b
木贼镰孢菌	25	80.0	17.5b
CK	25	0.0	0.0c

真菌	杀菌剂	毒力回归方程	EC₅₀/ (μg/mL)	相关系数(r)
尖孢镰孢菌	苯醚甲环唑	y=2.5348+1.1085x	167.39	0.9970
	丙环唑	y=2.3749+1.0447x	325.72	0.9742
	代森锰锌	y=2.0356+1.0980x	500.99	0.9764
	吡唑醚菌酯	y=1.6043+1.3486x	329.60	0.9965
	戊唑醇	y=2.1997+1.1430x	281.74	0.9833
茄病镰孢菌	苯醚甲环唑	y=3.4822+0.3781x	55.24	0.9941
	丙环唑	y=3.4575+0.6907x	171.05	0.9886
	代森锰锌	y=2.8249+0.8293x	419.46	0.9914
	吡唑醚菌酯	y=3.3318+0.8891x	75.21	0.9955
	戊唑醇	y=2.9935+0.9932x	104.81	0.9934
木贼镰孢菌	苯醚甲环唑	y=3.6599+0.8312x	40.93	0.9971
	丙环唑	y=3.1556+0.8572x	141.83	0.9880
	代森锰锌	y=3.3398+0.7003x	234.81	0.9852
	吡唑醚菌酯	y=1.8224+1.3759x	203.89	0.9705
	戊唑醇	y=3.1090+1.0058x	75.88	0.9642

真菌	杀菌剂	毒力回归方程	EC₅₀/ (μg/mL)	相关系数(r)
尖孢镰孢菌	苯醚甲环唑	y=2.5348+1.1085x	167.39	0.9970
	丙环唑	y=2.3749+1.0447x	325.72	0.9742
	代森锰锌	y=2.0356+1.0980x	500.99	0.9764
	吡唑醚菌酯	y=1.6043+1.3486x	329.60	0.9965
	戊唑醇	y=2.1997+1.1430x	281.74	0.9833
茄病镰孢菌	苯醚甲环唑	y=3.4822+0.3781x	55.24	0.9941
	丙环唑	y=3.4575+0.6907x	171.05	0.9886
	代森锰锌	y=2.8249+0.8293x	419.46	0.9914
	吡唑醚菌酯	y=3.3318+0.8891x	75.21	0.9955
	戊唑醇	y=2.9935+0.9932x	104.81	0.9934
木贼镰孢菌	苯醚甲环唑	y=3.6599+0.8312x	40.93	0.9971
	丙环唑	y=3.1556+0.8572x	141.83	0.9880
	代森锰锌	y=3.3398+0.7003x	234.81	0.9852
	吡唑醚菌酯	y=1.8224+1.3759x	203.89	0.9705
	戊唑醇	y=3.1090+1.0058x	75.88	0.9642

杀菌剂	平均b值	杀菌剂	平均b值
苯醚甲环唑	0.77b	吡唑醚菌酯	1.20a
丙环唑	0.86b	戊唑醇	1.05ab
代森锰锌	0.88b