MRP Protein Family of Foxtail Millet: Analysis of Sequence Characteristics, Molecular Evolution and Expression Pattern

doi:10.11923/j.issn.2095-4050.cjas2020-0059

Abstract

Abstract:

The aims are to study the gene structure and expression patterns of folate transport family members, and establish the foundation for exploring the molecular mechanism of folate transport in foxtail millet. Bioinformatics analysis of MRP family members was carried out with Phytozome, Clustal X, MEGA7.0 and other online tools and software. Based on the transcriptome sequencing data of foxtail millet, the expression patterns of foxtail millet MRP family members were analyzed. The results show that there are 21 foxtail millet MRP protein family members, most of which are distributed on chromosome 7, with 8 genes; 17 MRP proteins are alkaline and 4 proteins are acidic, and they are all hydrophilic proteins according to the hydrophobic value. The expression of SiMRP7 and SiMRP12 genes and the total folate content in foxtail millet tissue have a synergistic decrease trend, and these two proteins might play a regulatory role in the accumulation in foxtail millet. This study further identifies folate transport-related proteins, and could provide a theoretical basis for future research on folate metabolism pathway and gene mining.

Key words: Foxtail Millet, MRP Protein Family, Folate of Foxtail Millet, Bioinformatics Analysis, Expression Pattern

CLC Number:

S515

Gao Hao, Man Xiaxia, Sun Zhaoxia, Han Yuanhuai, Li Hongying, Hou Siyu, Guo Shujin. MRP Protein Family of Foxtail Millet: Analysis of Sequence Characteristics, Molecular Evolution and Expression Pattern[J]. Journal of Agriculture, 2021, 11(5): 74-82.

Figures/Tables 10

References 26

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基因名称	基因ID	基因序列长度/bp	氨基酸(aa)	等电点/PI	不稳定系数	平均疏水性
SiMRP1	Seita.1G055500	9385	1244	7.91	40.54	0.045
SiMRP2	Seita.1G055700	6343	1250	6.78	42.35	0.102
SiMRP3	Seita.1G190900	6878	1240	8.66	37.64	0.106
SiMRP4	Seita.1G191000	4984	1260	6.62	40.66	0.129
SiMRP5	Seita.1G191100	6138	635	8.54	38.26	0.137
SiMRP6	Seita.1G191200	4413	1214	8.59	40.46	0.136
SiMRP7	Seita.1G280600	11191	1257	9.32	39.36	0.118
SiMRP8	Seita.2G374400	5307	1259	8.34	42.34	0.072
SiMRP9	Seita.4G047900	15870	1465	7.46	41.88	0.276
SiMRP10	Seita.5G163300	17930	1609	7.18	38.02	0.180
SiMRP11	Seita.5G185300	9254	1447	8.25	38.46	0.179
SiMRP12	Seita.6G253500	7029	1355	8.91	38.00	0.099
SiMRP13	Seita.7G039700	8783	1530	5.86	37.72	0.211
SiMRP14	Seita.7G039900	7753	1544	5.38	37.76	0.161
SiMRP15	Seita.7G043600	9352	1521	8.91	36.72	0.078
SiMRP16	Seita.7G076700	7041	1539	8.69	38.90	0.028
SiMRP17	Seita.7G076800	7212	1534	8.46	40.60	0.026
SiMRP18	Seita.7G123300	6915	1258	8.56	36.32	0.155
SiMRP19	Seita.7G216700	8049	1603	8.31	42.73	0.044
SiMRP20	Seita.7G258700	6595	1264	8.46	35.65	0.127
SiMRP21	Seita.9G543800	6977	1507	8.48	45.28	0.175
SvNAC205	Sevir.9G576300.1.			9	55615117-55617062

基因名称	基因ID	基因序列长度/bp	氨基酸(aa)	等电点/PI	不稳定系数	平均疏水性
SiMRP1	Seita.1G055500	9385	1244	7.91	40.54	0.045
SiMRP2	Seita.1G055700	6343	1250	6.78	42.35	0.102
SiMRP3	Seita.1G190900	6878	1240	8.66	37.64	0.106
SiMRP4	Seita.1G191000	4984	1260	6.62	40.66	0.129
SiMRP5	Seita.1G191100	6138	635	8.54	38.26	0.137
SiMRP6	Seita.1G191200	4413	1214	8.59	40.46	0.136
SiMRP7	Seita.1G280600	11191	1257	9.32	39.36	0.118
SiMRP8	Seita.2G374400	5307	1259	8.34	42.34	0.072
SiMRP9	Seita.4G047900	15870	1465	7.46	41.88	0.276
SiMRP10	Seita.5G163300	17930	1609	7.18	38.02	0.180
SiMRP11	Seita.5G185300	9254	1447	8.25	38.46	0.179
SiMRP12	Seita.6G253500	7029	1355	8.91	38.00	0.099
SiMRP13	Seita.7G039700	8783	1530	5.86	37.72	0.211
SiMRP14	Seita.7G039900	7753	1544	5.38	37.76	0.161
SiMRP15	Seita.7G043600	9352	1521	8.91	36.72	0.078
SiMRP16	Seita.7G076700	7041	1539	8.69	38.90	0.028
SiMRP17	Seita.7G076800	7212	1534	8.46	40.60	0.026
SiMRP18	Seita.7G123300	6915	1258	8.56	36.32	0.155
SiMRP19	Seita.7G216700	8049	1603	8.31	42.73	0.044
SiMRP20	Seita.7G258700	6595	1264	8.46	35.65	0.127
SiMRP21	Seita.9G543800	6977	1507	8.48	45.28	0.175
SvNAC205	Sevir.9G576300.1.			9	55615117-55617062

基因名称	α螺旋	延伸链	β折叠	无规则卷曲
SiMRP1	52.33%	17.20%	5.71%	24.76%
SiMRP2	50.56%	17.04%	6.00%	26.40%
SiMRP3	51.05%	17.34%	6.61%	25.00%
SiMRP4	50.48%	16.90%	6.03%	26.59%
SiMRP5	48.98%	18.74%	9.92%	22.36%
SiMRP6	51.57%	17.22%	5.93%	25.29%
SiMRP7	51.79%	16.47%	5.73%	26.01%
SiMRP8	50.28%	17.24%	6.04%	26.45%
SiMRP9	55.70%	13.86%	4.30%	26.14%
SiMRP10	47.36%	17.09%	5.53%	30.02%
SiMRP11	54.32%	14.24%	5.53%	25.92%
SiMRP12	47.90%	15.42%	5.90%	30.77%
SiMRP13	51.31%	15.03%	5.29%	28.37%
SiMRP14	51.75%	15.28%	5.57%	27.40%
SiMRP15	51.74%	14.79%	5.33%	28.14%
SiMRP16	50.68%	15.27%	5.46%	28.59%
SiMRP17	50.26%	15.84%	5.35%	28.55%
SiMRP18	50.56%	16.38%	5.64%	27.42%
SiMRP19	48.66%	15.16%	5.36%	30.82%
SiMRP20	51.27%	16.06%	5.93%	26.74%
SiMRP21	53.28%	14.07%	5.64%	27.01%

基因名称	α螺旋	延伸链	β折叠	无规则卷曲
SiMRP1	52.33%	17.20%	5.71%	24.76%
SiMRP2	50.56%	17.04%	6.00%	26.40%
SiMRP3	51.05%	17.34%	6.61%	25.00%
SiMRP4	50.48%	16.90%	6.03%	26.59%
SiMRP5	48.98%	18.74%	9.92%	22.36%
SiMRP6	51.57%	17.22%	5.93%	25.29%
SiMRP7	51.79%	16.47%	5.73%	26.01%
SiMRP8	50.28%	17.24%	6.04%	26.45%
SiMRP9	55.70%	13.86%	4.30%	26.14%
SiMRP10	47.36%	17.09%	5.53%	30.02%
SiMRP11	54.32%	14.24%	5.53%	25.92%
SiMRP12	47.90%	15.42%	5.90%	30.77%
SiMRP13	51.31%	15.03%	5.29%	28.37%
SiMRP14	51.75%	15.28%	5.57%	27.40%
SiMRP15	51.74%	14.79%	5.33%	28.14%
SiMRP16	50.68%	15.27%	5.46%	28.59%
SiMRP17	50.26%	15.84%	5.35%	28.55%
SiMRP18	50.56%	16.38%	5.64%	27.42%
SiMRP19	48.66%	15.16%	5.36%	30.82%
SiMRP20	51.27%	16.06%	5.93%	26.74%
SiMRP21	53.28%	14.07%	5.64%	27.01%