Effects of Overexpression of StCYS1 on Potato Growth and Development and Enzymatic Browning

doi:10.11923/j.issn.2095-4050.cjas20200300067

Abstract

Abstract:

To explore the effect of cysteine protease inhibitor gene StCYS1 on the growth and development of potato plants and the enzymatic browning of tubers, StCYS1 overexpressed and wild type ‘MDS’ diploid potato plants were used as materials to study the photosynthetic index, root development, yield, browning of tubers after harvest, PPO activity, total antioxidant capacity and free amino acid content. Compared with wild-type potato plants, the photosynthetic capacity of overexpression lines was significantly improved, the root system was more developed and the yield was higher. Compared with the wild type, the overexpression lines showed less enzymatic browning, higher PPO activity, higher free proline content and total antioxidant capacity, and lower free tyrosine content. The results show that the overexpression of StCYS1 could significantly increase the photosynthesis of potato plants, promote the development of roots and plants, and then increase the yield of potato tubers. The overexpression of StCYS1 effectively improves the antioxidant capacity of potato tubers, reduces the content of free tyrosine, and significantly inhibits the enzymatic browning of potatoes.

Key words: Potato, StCYS1, Overexpression, Growth and Development, Enzymatic Browning

CLC Number:

S532

Liu Minmin, Li Guangcun, Liu Shiyang, Dong Tiantian, Wang Qingguo, Liu Pei. Effects of Overexpression of StCYS1 on Potato Growth and Development and Enzymatic Browning[J]. Journal of Agriculture, 2020, 10(6): 86-93.

Figures/Tables 9

References 32

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株系	总根长/cm	总表面积/cm²	平均直径/mm	总体积/cm³
WT	504.40±14.73d	156.43±4.32d	0.65±0.05c	2.18±0.97c
OE1	1003.43±12.06b	213.71±3.48b	0.76±0.06b	3.67±0.33b
OE2	1221.05±19.31a	292.25±8.33a	0.87±0.03a	6.87±0.91a
OE3	831.87±18.56c	199.30±7.29c	0.64±0.02c	3.73±0.28b

株系	总根长/cm	总表面积/cm²	平均直径/mm	总体积/cm³
WT	504.40±14.73d	156.43±4.32d	0.65±0.05c	2.18±0.97c
OE1	1003.43±12.06b	213.71±3.48b	0.76±0.06b	3.67±0.33b
OE2	1221.05±19.31a	292.25±8.33a	0.87±0.03a	6.87±0.91a
OE3	831.87±18.56c	199.30±7.29c	0.64±0.02c	3.73±0.28b

株系	单株薯重/g	单株结薯数量/个	最大单薯质量/g
WT	9.67±1.41d	8.80±0.74d	4.60
OE1	14.25±0.91b	23.33±1.55a	6.81
OE2	19.63±1.63a	17.86±1.47b	11.48
OE3	10.93±0.74c	10.33±0.34c	7.04

株系	单株薯重/g	单株结薯数量/个	最大单薯质量/g
WT	9.67±1.41d	8.80±0.74d	4.60
OE1	14.25±0.91b	23.33±1.55a	6.81
OE2	19.63±1.63a	17.86±1.47b	11.48
OE3	10.93±0.74c	10.33±0.34c	7.04

差异氨基酸种类	WT	OE1	OE2	OE3
天冬氨酸(Asp)	0.37±0.014b	0.41±0.020a	0.42±0.018a	0.42±0.008a
苏氨酸(Thr)	0.04±0.006c	0.13±0.003a	0.15±0.003a	0.10±0.009b
脯氨酸(Pro)	0.19±0.001c	0.25±0.002b	0.28±0.015a	0.28±0.011a
亮氨酸(Leu)	0.037±0.001c	0.043±0.001a	0.040±0.002b	0.041±0.002b
甘氨酸(Gly)	0.11±0.003a	0.04±0.001c	0.04±0.001c	0.06±0.002b
组氨酸(His)	0.055±0.002a	0.048±0.003b	0.051±0.001b	0.050±0.000b
酪氨酸(Tyr)	0.11±0.005a	0.03±0.002c	0.03±0.001bc	0.04±0.001b
缬氨酸(Val)	0.040±0.000a	0.010±0.001c	0.012±0.000b	0.012±0.001b
蛋氨酸(Met)	0.10±0.007a	0.03±0.002c	0.04±0.001b	0.03±0.003c
异亮氨酸(Ile)	0.06±0.005a	0.02±0.000c	0.03±0.001b	0.03±0.000b
苯丙氨酸(Phe)	0.08±0.011a	0.04±0.004c	0.05±0.003b	0.04±0.002c