F Type and Other Male Sterile Lines of Wheat: Molecular Identification of Cytoplasmic Types

doi:10.11923/j.issn.2095-4050.cjas2021-0143

Abstract

Abstract:

In order to identify the difference of cytoplasmic types between F type and other male sterile lines of wheat, the experiment was conducted with F type sterile line (FA1-1, FA1-2, FA2-1), F type maintainer line, the T, K and V type CMS lines (A) and the common maintainer line (B) ‘Ji5418’ as the materials, and 23 pairs of chloroplasts and 21 pairs of mitochondrial SSR markers were used for cluster analysis. The results showed that the cytoplasm of FA and T, K, V and B male sterile lines belonged to different categories. The cytoplasmic genetic similarity coefficient between FA and Chinese Spring (CS) was 1.00, and the cytoplasmic genetic similarity coefficient between FA and FB was also 1.00, indicating that FA might be a cytoplasmic male sterile line of common wheat. The fertility of F type male sterile line might be controlled by nuclear genes, which is different from the classical cytoplasmic-nuclear interaction male sterile principle.

Key words: wheat, F type male sterile line, chloroplast, mitochondria, SSR

CLC Number:

S336

REN Wenbin, WANG Qian, WU Cuicui, XIE Sangang. F Type and Other Male Sterile Lines of Wheat: Molecular Identification of Cytoplasmic Types[J]. Journal of Agriculture, 2022, 12(12): 1-5.

Figures/Tables 4

References 24

[1]	李峰, 曹丽蓉, 刘培园, 等. 小麦品种渝麦13号细胞质类型的分子标记鉴定[J]. 中国农业大学学报, 2018, 23(10):1-7.
[2]	GUPTA P K, BALYAN H S, GAHLAUT V, et al. Hybrid wheat: past, present and future[J]. Theoretical and applied genetics, 2019, 132:2463-2483. doi: 10.1007/s00122-019-03397-y pmid: 31321476
[3]	MUKAI Y, TSUNEWAKI K. Basic studies on hybrid wheat breeding[J]. Theoretical and applied genetics, 1979, 54(4):153-160. doi: 10.1007/BF00263045 URL
[4]	李传友, 孙兰珍. 普通小麦T型、V型和K型细胞质雄性不育系花粉败育机理的细胞学研究[J]. 华北农学报, 1996, 11(2):1-8. doi: 10.7668/hbnxb.1996.02.001
[5]	汪月, 张艳霞, 蔺艳丽, 等. 小麦Ven型胞质雄性不育植株与可育植株花药蛋白质组差异研究[J]. 西北植物学报, 2016, 36(6):1135-1145.
[6]	李罗江, 茹振刚, 高庆荣, 等. BNS小麦的雄性不育性及其温光特性[J]. 中国农业科学, 2009, 42(9):3019-3027.
[7]	孙辉, 张立平, 陈兆波, 等. BS型小麦光温敏雄性不育系光合特性研究[J]. 麦类作物报, 2020, 40(1):86-95.
[8]	倪飞. 太谷核不育基因MS2基因的图位克隆和功能解析[D]. 泰安: 山东农业大学, 2017.
[9]	张政值. 太谷核不育小麦(Triticum aestivum)雄性不育相关基因的克隆研究[D]. 南京: 南京农业大学, 2003.
[10]	柳娜, 杨文雄, 王世红, 等. 化学杀雄剂SQ-1诱导春小麦雄性不育的效果和对农艺性状的影响[J]. 作物杂志, 2014(1):23-25.
[11]	赵昌平. 中国杂交小麦研究现状与趋势[J]. 中国农业科技导报, 2010, 12(2):5-8.
[12]	CHEN Z W, NAN ZHAO N, LI S S, et al. Plant mitochondrial genome evolution and cytoplasmic male sterility[J]. Critical reviews in plant sciences, 2017, 36(1):55-69. doi: 10.1080/07352689.2017.1327762 URL
[13]	巴青松, 张改生, 李桂萍, 等. 小麦线粒体与细胞质雄性不育关系的研究进展[J]. 麦类作物学报, 2015, 35(1):16-21.
[14]	季道藩. 遗传学[M].中国农业出版社, 2000.
[15]	翁群珠. 几类小麦雄性不育系线粒体DNA的AFLP标记及SCAR转化[D]. 杨凌: 西北农林科技大学, 2014.
[16]	苏宁, 何兆峰, 欧平和, 等. 小麦属植物叶绿体基因组结构的比较分析[J]. 麦类作物报, 2020, 40(1):55-64.
[17]	温辉芹, 马惠英, 裴自友, 等. F型小麦不育系育性与杂种优势研究[J]. 山西农业科学, 2017, 45(8):1219-1224.
[18]	赵卜, 张向展, 郑炜君, 等. 型小麦雄性不育系小孢子发育的细胞学观察[J]. 麦类作物学报, 2015, 35(7):918-925.
[19]	陈林, 吴迪, 白文明, 等. F型小麦雄性不育系测恢研究及杂种优势分析[J]. 麦类作物学报, 2016, 36(4):420-425.
[20]	ISHII T, MORI N, OGIHARA Y. Evaluation of allelic diversity at chloroplast microsatellite loci among common wheat and its ancestral species[J]. TAG. Theoretical and applied genetics, 2001, 103:896-904. doi: 10.1007/s001220100715 URL
[21]	ISHII T, TAKAHASHI C, IKEDA N, et al. Mitochondrial microsatellite variability in common wheat and its ancestral species[J]. Genes & genetic systems, 2006, 81:211-214.
[22]	NEI M, LI W H. Mathematical model for studying genetic variation in term of restriction endonucleases[J]. Proceedings of the National Academy of science USA, 1979, 76:5269-5273. doi: 10.1073/pnas.76.10.5269 URL
[23]	ANDERSON J A, CHURCHILL G A, AUTRIQUE J E, et al. Optimizing parental selection for genetic linkage maps[J]. Genome, 1993, 36:181-186. doi: 10.1139/g93-024 pmid: 18469981
[24]	袁凯, 张婷, 史晓芳, 等. K、V、T型小麦细胞质雄性不育系叶绿体DNA的SSR分析及RuBP羧化酶活性比较[J]. 西北植物学报, 2019, 39(3):472-479.

引物名称	等位位点数	PIC	引物名称	等位位点数	PIC
WCt2	2	0.172	WCt13	3	0.490
WCt3	2	0.172	WCt15	2	0.408
WCt4	3	0.431	WMt8	2	0.361
WCt10	2	0.172	WMt11	2	0.444
WCt11	3	0.431	WMt15	3	0.490

引物名称	等位位点数	PIC	引物名称	等位位点数	PIC
WCt2	2	0.172	WCt13	3	0.490
WCt3	2	0.172	WCt15	2	0.408
WCt4	3	0.431	WMt8	2	0.361
WCt10	2	0.172	WMt11	2	0.444
WCt11	3	0.431	WMt15	3	0.490