Abstract: In order to improve the quality of apple and its nutritional value, this study continuously measured the antioxidant activity and aging related indicators of the fresh detached adult leaves from Fuji apple trees every week in one month. The leaves were cultured in vitro with bottoms soaked in the solution of the same concentration of 1 mg/L kinetin, zeatin and 6-benzyl adenine. The results showed that: the total antioxidant activity of the leaves was improved by all three treatments, as well as the content of chlorophyll and the protein of the leaves. However, the content of argentine and proline declined. The kinetin treatment had the most obvious effects on improving the antioxidant activity and chlorophyll content. The zeatin treatment had the most significant effects on increasing the content of protein and reducing the content of amino acids. The 6-benzyl adenine maintained a medium-level impact. The increase of total antioxidant activity and antiaging effects through applying different plant cytokinin provided theoretical foundation for the production of functional healthy apples of rich antioxidant activity materials.