Welcome to Journal of Agriculture,

Journal of Agriculture ›› 2021, Vol. 11 ›› Issue (8): 14-21.doi: 10.11923/j.issn.2095-4050.cjas20200200027

Special Issue: 生物技术 小麦

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Genetic Transformation System of Different Genotypes of Buckwheat: A Preliminary Study

Wang Xinfang1(), Feng Jinfang1, Hou Siyu1, Feng Jinhua1, Du Wei1, Feng Hongmei1, Han Yuanhuai1, Liu Longlong2, Ma Mingchuan2, Wang Junzhen3, Sun Zhaoxia1, Li Hongying1()   

  1. 1Agricultural College of Shanxi Agricultural University, Taigu 030801, Shanxi, China
    2Institute of Germplasm Resources, Shanxi Academy of Agriculture Science, Taiyuan 030031, Shanxi, China
    3Liangshan Xichang Institute of Agricultural Science, Xichang 615000, Sichuan, China
  • Received:2020-02-18 Revised:2020-03-22 Online:2021-08-20 Published:2021-08-26
  • Contact: Li Hongying E-mail:3123676106@qq.com;HONGYING1964@163.com

Abstract:

The aim is to utilize the basis of genetic transformation to break the bottleneck of buckwheat hybridization and germplasm innovation, and improve the research level of buckwheat. In this experiment, common buckwheat ‘PI647061’ and tartary buckwheat ‘ZNQ152’ were used as materials, and the hypocotyls were cultured by orthogonal experiment. The callus was infected by agrobacterium-mediated transformation, and the temporary expression of GUS gene was observed by histochemical staining. The results showed that MS + 2.0 mg/L 2,4-D + 1.0 mg/L 6-BA could make the calli ratio of hypocotyls reach more than 90%. In MS + 0.5 mg/L NAA + 1.0 mg/L 6-BA, hypocotyls of ten species of buckwheat had the highest differentiation rates, and the highest regeneration rate was 52.6%. The calli were infected by agrobacterium tumefaciens LBA4404, which carried the genetic transformation vector pRI201-AN-GUS. And the regenerated seedlings were obtained after selective culture, and then the blue spots of GUS gene expression were successfully observed in the leaves. The positive rate of transformation was 1.23%. This study provides a theoretical and practical basis for the exploration of the transformation of buckwheat excellent genes and the establishment of an effective genetic regeneration system.

Key words: Buckwheat, Plant Hormones, Callus, Orthogonal Experiment, Genetic Transformation

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