Sensitivity of Corynespora cassiicola Causing Cucumber Corynespora Spot to Boscalid in Tianjin

doi:10.11923/j.issn.2095-4050.cjas2020-0042

Abstract

Abstract:

To investigate the sensitivity level of Corynespora cassiicola to boscalid, a new carboxamide, 102 isolates of C. cassiicola in Tianjin were evaluated by mycelial growth rate method. The results showed that there existed significant difference in the sensitivity of C. cassiicola. The minimum value of EC₅₀ was 0.29 μg/mL, and the maximum value was 773.33 μg/mL. The total resistance frequency of the 102 strains to boscalid was 78.43%, and the high resistance frequency was 4.9%. The application frequency of boscalid in cucumber production in Tianjin is relatively high, and it has moderate resistance risk, which is not suitable for the control of Corynespora leaf spot in Tianjin.

Key words: Cucumber, Corynespora cassiicola, Boscalid, Sensitivity, Resistance Level

CLC Number:

S482.2

Gao Wei, Yang Lijuan, Wang Yong, Ma Jianfang, Liu Yaquan. Sensitivity of Corynespora cassiicola Causing Cucumber Corynespora Spot to Boscalid in Tianjin[J]. Journal of Agriculture, 2021, 11(9): 20-23.

Figures/Tables 2

References 24

[1]	张自心, 谢学文, 傅俊范, 等. 黄瓜棒孢叶斑病病原学和抗性基因研究进展[J]. 生物技术进展, 2016, 6(3):169-173.
[2]	李晗, 冉茂, 陈海涛, 等. 植物棒孢霉叶斑病的发生及防治研究进展[J]. 植物医生, 2020, 33(1):15-20.
[3]	吴桥, 赵秀香, 吴元华. 辽、冀、鲁黄瓜褐斑病菌(Corynespora cassiicola)培养性状及致病力分化研究[J]. 沈阳农业大学学报, 2017, 48(2):207-212.
[4]	张艳菊, 刘奇, 刘东, 等. 黄瓜棒孢叶斑病菌的毒力分化及AFLP分析[J]. 北方园艺, 2016(16):92-96,98.
[5]	李淑菊, 王惠哲, 杨瑞环, 等. 黄瓜棒孢叶斑病菌基因组ISSR分子指纹分析[J]. 分子植物育种, 2015, 13(11):2531-2536.
[6]	李俊香, 洪霓, 古勤生. 22种杀菌剂对甜瓜靶斑病菌的室内毒力测试[J]. 中国瓜菜, 2018, 31(9):13-16.
[7]	禾丽菲,, 李晓旭, 朱佳美, 等. 不同杀菌剂对黄瓜靶斑病菌的毒力作用特性比较[J]. 农药学学报, 2018, 20(1):25-32.
[8]	温浩, 张桂军, 毕扬, 等. 草莓棒孢叶斑病的病原菌鉴定及其对9种杀菌剂的敏感性[J]. 农药学学报, 2019, 21(3):279-284.
[9]	MacKenzie K J, Xavier K V, Wen A, et al. Widespread QoI Fungicide Resistance Revealed Among Corynespora cassiicola Tomato Isolates in Florida.[J]. Plant disease, 2020, 104(3):893-903. doi: 10.1094/PDIS-03-19-0460-RE pmid: 31891552
[10]	农业农村部农药鉴定所. 中国农药信息网[EB/OL].(2020-04-08). http://www.chinapesticide.gov.cn/.
[11]	张慧丽, 张文, 康永利, 等. 新型烟酰胺类杀菌剂啶酰菌胺的合成[J]. 农药, 2016, 55(7):491-492,503.
[12]	华乃震. SDHI类杀菌剂啶酰菌胺[J]. 世界农药, 2018, 40(5):9-15.
[13]	Ruprecht J, Yankovskaya V, Maklashina E, et al. Structure of Escherichia coli succinate: quinone oxidoreductase with an occupied and empty quinone-binding site[J]. J Biol Chem, 2009, 284:29836-29846. doi: 10.1074/jbc.M109.010058 URL
[14]	于乐祥, 张秀珍. 新型烟酰胺类杀菌剂啶酰菌胺的研究进展[J]. 山东农药信息, 2009(11):21.
[15]	Ishii H, Miyamoto T, Ushio S, et al. Lack of cross-resistance to a novel succinate dehydrogenase inhibitor, fluopyram, in highly boscalid-resistant isolates of Corynespora cassiicola and Podosphaera xanthii[J]. Pest management science, 2011, 67(4):474-482. doi: 10.1002/ps.2092 URL
[16]	Ishii H, Yano K, Date H., et al. Molecular characterization and diagnosis of QoI resistance in cucumber and eggplant fungal pathogens[J]. Phytopathology, 2007, 97:1458-1466. doi: 10.1094/PHYTO-97-11-1458 pmid: 18943516
[17]	Miyamoto T, Ishii H, Seko T, et al. Occurrence of Corynespora cassiicola isolates resistant to boscalid on cucumber in Ibaraki Prefecture, Japan[J]. Plant Pathology, 2009, 58(6):1144-1151. doi: 10.1111/ppa.2009.58.issue-6 URL
[18]	朱发娣. 黄瓜多主棒孢菌(Corynespora cassiicola)对啶酰菌胺的抗性及其机理研究[D]. 北京: 中国农业科学院, 2018.
[19]	Zhu F D, Shi Y X, Xie X W, et al. Occurrence, distribution and characteristics of boscalid-resistant Corynespora cassiicola in China[J]. Plant Disease, 2019, 103:69-76 doi: 10.1094/PDIS-11-17-1760-RE URL
[20]	Miyamoto T, Ishii H, Stammler G, et al. Distribution and molecular characterization of Corynespora cassiicola isolates resistant to boscalid[J]. Plant Pathology, 2010, 59(5):873-881. doi: 10.1111/ppa.2010.59.issue-5 URL
[21]	纪军建, 张小风, 王文桥, 等. 黄瓜褐斑病化学药剂防治研究进展[J]. 河北农业科学, 2010, 14(8):28-31.
[22]	李宝聚, 高苇, 石延霞, 等. 多主棒孢和棒孢叶斑病的研究进展[J]. 植物保护学报, 2012, 39(2):171-176.
[23]	于淑晶, 王满意, 田芳, 等. 黄瓜棒孢叶斑病的防治及抗药性研究进展[J]. 农药, 2014, 53(1):7-11,33.
[24]	白滨, 何苏琴, 荆卓琼, 等. 分离自黄瓜的多主棒孢霉不同表型菌株对杀菌剂的敏感性[J]. 微生物学通报, 2016, 43(2):322-329.

采样地区	菌株数量	EC₅₀/(μg/mL)		不同抗性水平菌株频率/%				抗性频率/%
采样地区	菌株数量	范围	均值±标准差	敏感(S)	低抗(LR)	中抗(MR)	高抗(HR)	抗性频率/%
西青	18	4.10~773.33	69.99±177.42	16.67	55.56	22.22	5.56	83.33
武清	21	0.35~121.52	42.92±32.55	14.29	38.10	42.86	4.76	85.71
宝坻	13	1.94~482.89	64.22±129.28	30.77	38.46	23.08	7.69	69.23
北辰	8	5.49~92.71	34.98±29.32	25.00	50.00	25.00	0.00	75.00
静海	36	0.29~240.76	42.87±48.88	19.44	50.00	25.00	5.56	80.56
宁河	6	3.56~65.56	18.07±23.73	50.00	33.33	16.67	0.00	50.00
总体情况	102	0.29~773.33	48.31±92.80	21.57	46.08	27.45	4.90	78.43

采样地区	菌株数量	EC₅₀/(μg/mL)		不同抗性水平菌株频率/%				抗性频率/%
采样地区	菌株数量	范围	均值±标准差	敏感(S)	低抗(LR)	中抗(MR)	高抗(HR)	抗性频率/%
西青	18	4.10~773.33	69.99±177.42	16.67	55.56	22.22	5.56	83.33
武清	21	0.35~121.52	42.92±32.55	14.29	38.10	42.86	4.76	85.71
宝坻	13	1.94~482.89	64.22±129.28	30.77	38.46	23.08	7.69	69.23
北辰	8	5.49~92.71	34.98±29.32	25.00	50.00	25.00	0.00	75.00
静海	36	0.29~240.76	42.87±48.88	19.44	50.00	25.00	5.56	80.56
宁河	6	3.56~65.56	18.07±23.73	50.00	33.33	16.67	0.00	50.00
总体情况	102	0.29~773.33	48.31±92.80	21.57	46.08	27.45	4.90	78.43