Comparison of the Toxicity of Four Anthranilic Diamide Insecticides to Different Phase of Spodoptera frugiperda

doi:10.11923/j.issn.2095-4050.cjas2023-0021

Abstract

Abstract:

In order to compare the sensitivity of four anthranilic diamide insecticides against different phase of Spodoptera frugiperda, egg-dip, leaf-dip and poison nutrient solution-feeding methods were used to test the toxicity of chlorantraniliprole, flubendiamide, tetrachlorantraniliprole and cyantraniliprole on eggs, 3^rd-larvae and adults. The results indicated that there were no ovicidal activities in the four anthranilic diamide insecticides, but could significantly reduce the survival rates of neonate larvae. The sensitivity of tetrachlorantraniliprole to 3^rd instar larvae was the highest, the LC₅₀ was 0.12 mg/L, followed by chlorantraniliprole, flubendiamide, which were 33.83, 5.21, 2.24 times of the toxicity of cyantraniliprole, respectively. The most sensitive to adults was chlorantraniliprole, which was 6.20 mg/L, followed by tetrachlorantraniliprole and cyantraniliprole, and the least sensitive was flubendiamide, which was 25.96 mg/L. The sensitivity of S. frugiperda in different phase to four anthranilic diamide insecticides had been tested by using three methods, which would provide basic data for the chemical control and resistance management of S. frugiperda.

Key words: Spodoptera frugiperda, chlorantraniliprole, flubendiamide, tetrachlorantraniliprole, cyantraniliprole, toxicity

ZHOU Chao, ZHANG Yong, LIANG Chao, MA Chong, YANG Lina, REN Hong. Comparison of the Toxicity of Four Anthranilic Diamide Insecticides to Different Phase of Spodoptera frugiperda[J]. Journal of Agriculture, 2023, 13(12): 34-38.

Figures/Tables 3

References 28

[1]	中华人民共和国国家统计局. 主要农作物播种面积年度数据[EB/OL]. https://data.stats.gov.cn/easyquery.htm?cn=C01.2023-04-18/2023-04-18.
[2]	中华人民共和国国家统计局. 主要农作物产品产量年度数据[EB/OL]. https://data.stats.gov.cn/easyquery.htm?cn=C01.2023-04-18/2023-04-18.
[3]	王芹芹, 崔丽, 王立, 等. 草地贪夜蛾对杀虫剂的抗性研究进展[J]. 农药学学报, 2019, 21(4):401-408.
[4]	TODD E L, POOLE R W. Keys and illustrations for the armyworm moths of the noctuid genus Spodoptera Guenée from the Western Hemisphere[J]. Annals of the entomological society of America, 1980, 73(6):722-738. doi: 10.1093/aesa/73.6.722 URL
[5]	郭井菲, 赵建周, 何康来, 等. 警惕危险性害虫草地贪夜蛾入侵中国[J]. 植物保护, 2018, 44(6):1-10.
[6]	姜玉英, 刘杰, 朱晓明. 草地贪夜蛾侵入我国的发生动态和未来趋势分析[J]. 中国植保导刊, 2019, 39(2):33-35.
[7]	姜玉英, 刘杰, 谢茂昌, 等. 2019年我国草地贪夜蛾扩散为害规律观测[J]. 植物保护, 2019, 45(6):10-19.
[8]	CHAPMAN D, PURSE B V, ROY H E, et al. Global trade networks determine the distribution of invasive non-native species[J]. Global ecology biogeography, 2017, 26(8):907-917. doi: 10.1111/geb.2017.26.issue-8 URL
[9]	COCK M J W, BESEH P K, BUDDIE A G, et al. Molecular methods to detect Spodoptera frugiperda in Ghana, and implications for monitoring the spread of invasive species in developing countries[J]. Scientific reports, 2017, 7(1):4103. doi: 10.1038/s41598-017-04238-y
[10]	太红坤, 郭井菲, 张峰, 等. 草地贪夜蛾在云南冬季甜玉米上的生物学习性及为害状观察[J]. 植物保护, 2019, 45(5):91-95.
[11]	SATTELLE DAVID B, CORDOVA DANIEL, CHEEK TIMOTHY R. Insect ryanodine receptors: Molecular targets for novel pest control chemicals[J]. Invertebrate neuroscience, 2008, 8(3):107-119. doi: 10.1007/s10158-008-0076-4 pmid: 18696132
[12]	李秀霞, 梁沛, 高希武. 昆虫对双酰胺类杀虫剂抗性机制研究进展[J]. 植物保护学报, 2015, 42(4):481-487.
[13]	赵平, 严秋旭, 李新, 等. 双酰胺类杀虫剂的现状与展望[J]. 农药科学与管理, 2015, 36(11):23-29.
[14]	陈琴, 涂红, 邰安琪, 等. 双酰胺类杀虫化合物的研究进展[J]. 精细化工中间体, 2016, 46(2):11-16.
[15]	张琳, 张紫溪, 陈秀, 等. 双酰胺类农药生物活性、生态毒性及残留行为研究进展[J]. 农药学学报, 2023, 25(2):295-309.
[16]	郑雪松, 赖添财, 时立波, 等. 双酰胺类杀虫剂应用现状[J]. 农药, 2012, 51(8):554-557,580.
[17]	陈小平, 柳爱平, 关少飞, 等. 双酰胺类杀虫剂及其应用市场与防治刺吸式口器害虫前景[J]. 世界农药, 2021, 43(11):1-12.
[18]	李子园, 戴钎萱, 邝昭琅, 等. 3种人工饲料对草地贪夜蛾生长发育及繁殖力的影响[J]. 环境昆虫学报, 2019, 41(6):1147-1154.
[19]	张友军, 王光锋, 吴青君, 等. 多杀菌素对不同发育阶段甜菜夜蛾的毒力及其体内超氧化物歧化酶、过氧化氢酶和过氧化物酶的影响[J]. 农药学学报, 2003, 5(3):31-38.
[20]	刘永杰, 沈晋良. 甜菜夜蛾对氯氟氰菊酯抗性的表皮穿透机理[J]. 昆虫学报, 2003, 46(3):288-291.
[21]	鲁艳辉, 田俊策, 郑许松, 等. 室内测定6种化学杀虫剂对草地贪夜蛾幼虫的毒力[J]. 环境昆虫学报, 2020, 42(2):329-334.
[22]	王欢欢, 吕圣兰, 赵瑞, 等. 草地贪夜蛾幼虫对常用杀虫剂相对敏感基线的建立[J]. 昆虫学报, 2021, 64(12):1427-1432.
[23]	符伟, 张才建, 瞿玉国, 等. 氯虫苯甲酰胺与甲维盐复配对玉米田草地贪夜蛾的防效[J]. 湖南农业科学, 2022, 447(12):55-58.
[24]	尹艳琼, 张红梅, 李永川, 等. 8种杀虫剂对云南不同区域草地贪夜蛾种群的室内毒力测定[J]. 植物保护, 2019, 45(6):70-74.
[25]	陈澄宇, 赵云霞, 唐艺婷, 等. 7种杀虫剂对不同地区草地贪夜蛾田间种群的毒力测定[J]. 现代农药, 2022, 21(3):64-68.
[26]	杨帆, 望勇, 骆海波, 等. 13种化学杀虫剂对草地贪夜蛾的室内杀卵活性和田间防效[J]. 江苏农业科学, 2022, 50(8):37-42.
[27]	谢显彪, 沈云峰, 杨祚斌, 等. 6种药剂拌沙撒芯对干旱地区玉米草地贪夜蛾的防治效果[J]. 现代农药, 2022, 21(6):62-65.
[28]	徐永伟, 张如意, 杨翠丽, 等. 几种杀虫剂无人机喷雾对玉米田草地贪夜蛾的防治效果试验与分析[C]. 河南省植物保护学会,河南省昆虫学会,河南省植物病理学会.河南省植物保护学会第十二次、河南省昆虫学会第十一次、河南省植物病理学会第六次会员代表大会暨学术讨论会论文集.

药剂	浓度/(mg/L)	孵化率/%	初孵幼虫存活率/%
氯虫苯甲酰胺 Chlorantraniliprole	10	92.88aA	10.03cD
	2	90.84aA	17.16cCD
	0.4	91.54aA	30.24bBC
	0.08	92.43aA	43.70bB
	0.016	91.08aA	65.45aA
	0	93.19aA	74.72aA
氟苯虫酰胺 Flubendiamide	10	91.33aA	24.79eD
	2	92.29aA	35.71dCD
	0.4	91.48aA	46.16cBC
	0.08	90.67aA	59.18bB
	0.016	91.40aA	72.72aA
	0	93.25aA	73.46aA
四氯虫酰胺 Tetrachlorantraniliprole	10	91.53aA	1.81dD
	2	90.94aA	3.70dD
	0.4	93.23aA	19.05cC
	0.08	92.27aA	30.08cC
	0.016	92.48aA	49.49bB
	0	92.38aA	72.27aA
溴氰虫酰胺 Cyantraniliprole	10	91.34aA	21.72eD
	2	92.79aA	39.58dC
	0.4	93.05aA	52.77cB
	0.08	90.56aA	62.11bAB
	0.016	90.08aA	69.50abA
	0	92.48aA	73.02aA

药剂	浓度/(mg/L)	孵化率/%	初孵幼虫存活率/%
氯虫苯甲酰胺 Chlorantraniliprole	10	92.88aA	10.03cD
	2	90.84aA	17.16cCD
	0.4	91.54aA	30.24bBC
	0.08	92.43aA	43.70bB
	0.016	91.08aA	65.45aA
	0	93.19aA	74.72aA
氟苯虫酰胺 Flubendiamide	10	91.33aA	24.79eD
	2	92.29aA	35.71dCD
	0.4	91.48aA	46.16cBC
	0.08	90.67aA	59.18bB
	0.016	91.40aA	72.72aA
	0	93.25aA	73.46aA
四氯虫酰胺 Tetrachlorantraniliprole	10	91.53aA	1.81dD
	2	90.94aA	3.70dD
	0.4	93.23aA	19.05cC
	0.08	92.27aA	30.08cC
	0.016	92.48aA	49.49bB
	0	92.38aA	72.27aA
溴氰虫酰胺 Cyantraniliprole	10	91.34aA	21.72eD
	2	92.79aA	39.58dC
	0.4	93.05aA	52.77cB
	0.08	90.56aA	62.11bAB
	0.016	90.08aA	69.50abA
	0	92.48aA	73.02aA

药剂	调查时间/h	斜率(±SE)	LC₅₀(95%CL)/(mg/L)	R₁	R₂	R₃
氯虫苯甲酰胺Chlorantraniliprole	24	0.41±0.04	1213.39(338.44~4350.33)	3.57	-	-
	48	0.29±0.03	10.83(5.02~23.39)	-	5.22	-
	72	0.39±0.05	0.78(0.34~1.75)	-	-	5.21
氟苯虫酰胺Flubendiamide	24	0.48±0.02	1123.86(666.78~1894.26)	3.85	-	-
	48	0.36±0.04	30.80(10.04~94.45)	-	1.84	-
	72	0.48±0.01	1.81(1.54~2.13)	-	-	2.24
四氯虫酰胺Tetrachlorantraniliprole	24	0.49±0.05	4327.27(974.57~19213.53)	1.00	-	-
	48	0.37±0.02	1.12(0.83~1.52)	-	50.48	-
	72	0.48±0.07	0.12(0.04~0.34)	-	-	33.83
溴氰虫酰胺Cyantraniliprole	24	0.60±0.04	402.63(194.04~835.44)	10.75	-	-
	48	0.58±0.03	56.54(30.71~104.10)	-	1.00	-
	72	0.65±0.04	4.06(2.70~6.12)	-	-	1.00

药剂	调查时间/h	斜率(±SE)	LC₅₀(95%CL)/(mg/L)	R₁	R₂	R₃
氯虫苯甲酰胺Chlorantraniliprole	24	0.41±0.04	1213.39(338.44~4350.33)	3.57	-	-
	48	0.29±0.03	10.83(5.02~23.39)	-	5.22	-
	72	0.39±0.05	0.78(0.34~1.75)	-	-	5.21
氟苯虫酰胺Flubendiamide	24	0.48±0.02	1123.86(666.78~1894.26)	3.85	-	-
	48	0.36±0.04	30.80(10.04~94.45)	-	1.84	-
	72	0.48±0.01	1.81(1.54~2.13)	-	-	2.24
四氯虫酰胺Tetrachlorantraniliprole	24	0.49±0.05	4327.27(974.57~19213.53)	1.00	-	-
	48	0.37±0.02	1.12(0.83~1.52)	-	50.48	-
	72	0.48±0.07	0.12(0.04~0.34)	-	-	33.83
溴氰虫酰胺Cyantraniliprole	24	0.60±0.04	402.63(194.04~835.44)	10.75	-	-
	48	0.58±0.03	56.54(30.71~104.10)	-	1.00	-
	72	0.65±0.04	4.06(2.70~6.12)	-	-	1.00

药剂	斜率(±SE)	LC₅₀(95%CL)/(mg/L)	R₄
氯虫苯甲酰胺 Chlorantraniliprole	0.63±0.02	6.20(5.22~7.37)	4.19
氟苯虫酰胺 Flubendiamide	0.57±0.01	25.96(23.19~29.05)	1.00
四氯虫酰胺 Tetrachlorantraniliprole	0.61±0.03	6.28(4.15~9.52)	4.13
溴氰虫酰胺 Cyantraniliprole	0.60±0.01	20.75(18.49~23.29)	1.25