Abstract: To provide a technological basis for the high-yield cultivation of‘Koshihikari-S’, the effects of different applying patterns and amounts of fertilizer-N on growth and yield of‘Koshihikari-S’were researched to find out the reasonable pattern and suitable amount of fertilizer-N application. The experiment was done with a randomized block design with the Japanese high quality rice variety‘Koshihikari-S’as the material. There were 9 treatments in total with three replications: 2 kinds of applying patterns, 5 levels of applying amounts. The results showed that: for the new applying pattern of fertilizer-N (1:1:1:1), the yield (6935.7 kg/hm2) was the highest when fertilizer-N was applied for 202.5 kg/hm2 totally; for the traditional applying pattern of fertilizer-N (5:2:3:0), the yield (6534.3 kg/hm2) was the highest when fertilizer-N was applied for 135 kg/hm2 cultivated with the new pattern had less amount of growth in the early period, but grew steadily in the later period, thereby resisted lodging better; prolonged the functional period of leaves and increased the percentage of available tillers, seed setting rate and 1000-grain weight.‘Koshihikari-S’cultivated with the traditional pattern had the advantage of growing early and quickly thus increased spikes number, but had easy lodging and premature, and the percentage of available tillers, seed setting rate and 1000-grain weight reduced too. In conclusion, the reasonable applying pattern of fertilizer-N for the high-yield cultivation of‘Koshihikari-S’was: base fertilizer: tiller fertilizer : panicle fertilizer: grain fertilizer=1:1:1:1, the suitable applying amount of fertilizer-N was about 200 kg/hm2; the reasonable applying pattern of fertilizer- N for the mid- low- yield cultivation of‘Koshihikari- S’was: base fertilizer : tiller fertilizer : panicle fertilizer : grain fertilizer=5:2:3:0, the suitable applying amount of fertilizer-N was about 140 kg/hm2.