Comparison Study of 6 Kinds of Tomato Leaf Epidermis Slice Techniques

doi:10.11923/j.issn.2095-4050.cjas2021-0093

Abstract

Abstract:

This paper adopted six kinds of leaf epidermis slice techniques to treat lower epidermis of tomato leaves considering their structural characteristics, and compared the preparation process and observation effect of different techniques. The results show that tomato leaves are thin, the epidermis is single-layer cell with abundant epidermal hairs, and the lower epidermis is adjacent to the loose spongy tissue. Static characteristics, such as dynamic behavior of stomatal size, density and stomatal aperture, could be observed by skin peeling method and cellophane tape sticking method. Due to the softness of tomato leaves, the scraping method is extremely demanding to operate, and dissociation method is time-consuming and has poor effect, both the techniques are not suitable for the slice production of tomato leaf epidermis. Tearable nail oil blotting method could obtain the imprint of stomatal index and stomatal aperture, while the leaves would be damaged certainly. Silica gel-nail polish two-stage blotting method has no damage to the leaves, the same part of the same leaf could be imprinted continuously without scathe, and in this way, the dynamic behavior like stomatal movement and stomatal development could be observed continuously. The results could provide a basis for selecting suitable tomato leaf epidermis preparation methods for different research purposes.

Key words: Tomato, Leaf Epidermis Slice Techniques, Stoma, Microscopic Observation, Method Comparison and Improvement

CLC Number:

Q94-3

SONG Yanbo, YANG Yunhao, DUAN Pengjun, WANG Jiahao, WEI Siqing, ZHANG Yalong. Comparison Study of 6 Kinds of Tomato Leaf Epidermis Slice Techniques[J]. Journal of Agriculture, 2022, 12(1): 45-52.

Figures/Tables 6

References 27

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方法	时间/个	难度	取样面积占叶面积百分数/%	定位性	清晰度	气孔/表皮毛真实性	气孔密度/开度与指数观测
表皮撕取法	0.5~2	+++	0.9~2.5	++	++	+++	+++
透明胶带法	3~6	+	>90	+++	+++	+++	+++
指甲油印迹法	30	+	>90	+++	++	+	++
硅胶-指甲油印迹法	40	+	>90	+++	++	+	++
解离法	40	++	0.5~1.5	+	++	++	++
刮片法	0.5~2	+++	0.7~2.0	++	+	+++	+

方法	时间/个	难度	取样面积占叶面积百分数/%	定位性	清晰度	气孔/表皮毛真实性	气孔密度/开度与指数观测
表皮撕取法	0.5~2	+++	0.9~2.5	++	++	+++	+++
透明胶带法	3~6	+	>90	+++	+++	+++	+++
指甲油印迹法	30	+	>90	+++	++	+	++
硅胶-指甲油印迹法	40	+	>90	+++	++	+	++
解离法	40	++	0.5~1.5	+	++	++	++
刮片法	0.5~2	+++	0.7~2.0	++	+	+++	+