Effects of Different Humic Acid Contents in Substrates on Growth and Root Morphological Characteristics of Tobacco Seedling

doi:10.11923/j.issn.2095-4050.cjas2020-0183

Abstract

Abstract:

To explore the application effects of humic acid on tobacco seedling, different amounts of humic acid (0, 0.5, 1.0, 1.5, 2.0 g/plant) were added to the substrate and the growth and morphological characteristics of tobacco seedlings were studied with the method of tray seedling culture. The results showed that the tobacco seedling rate, strong seedling index, biomass, plant height, stem diameter, leaf length and width increased first and then decreased with the increase of humic acid in the substrate, and reached the maximum in the treatment of 1.0 to 1.5 g/plant. And the seedling rate and the strong seedling index reached 92.3% and 1.05, increased by 3.6 percentage points and 16.7% compared with those of the control, separately. In addition, humic acid in the substrate improved the root surface area, average diameter, root volume and the total length of roots at all levels. According to the comprehensive comparison, humic acid could effectively improve the root morphological characteristics and the overall quality of tobacco seedlings, and the optimal amount of humic acid added in the substrate is 1.0-1.5 g/plant (the total content of pure humic acid is 28.8%-34.4%).

Key words: humic acid, substrate, tobacco seedling, strong seedling index, root morphology

CLC Number:

S572

FAN Jun, WANG Rui, XU Dabing, TAN Jun. Effects of Different Humic Acid Contents in Substrates on Growth and Root Morphological Characteristics of Tobacco Seedling[J]. Journal of Agriculture, 2022, 12(7): 45-49.

Figures/Tables 6

References 25

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处理	株高/cm	茎粗/cm	叶数/片	叶长/cm	叶宽/cm
T₁	20.5±0.8a	3.90±0.21b	5.0±0.0a	15.1±0.1b	7.25±0.35ab
T₂	21.4±0.2a	4.35±0.21ab	5.0±0.0a	15.9±0.2a	7.45±0.07ab
T₃	21.6±1.4a	4.55±0.64a	5.0±0.0a	15.5±0.7ab	7.75±0.35a
T₄	20.0±0.9a	4.05±0.07b	5.0±0.0a	15.3±0.4ab	7.05±0.35b
T₅	17.5±1.3b	3.25±0.07c	5.0±0.0a	13.5±0.7c	6.55±0.07c

处理	株高/cm	茎粗/cm	叶数/片	叶长/cm	叶宽/cm
T₁	20.5±0.8a	3.90±0.21b	5.0±0.0a	15.1±0.1b	7.25±0.35ab
T₂	21.4±0.2a	4.35±0.21ab	5.0±0.0a	15.9±0.2a	7.45±0.07ab
T₃	21.6±1.4a	4.55±0.64a	5.0±0.0a	15.5±0.7ab	7.75±0.35a
T₄	20.0±0.9a	4.05±0.07b	5.0±0.0a	15.3±0.4ab	7.05±0.35b
T₅	17.5±1.3b	3.25±0.07c	5.0±0.0a	13.5±0.7c	6.55±0.07c

处理	总根长度/cm	总表面积/cm²	平均直径/mm	根系体积/cm³	根尖末端数/个
T₁	226.7±43.6a	47.17±14.09a	0.653±0.089ab	0.789±0.312b	793.0±73.0bc
T₂	233.3±27.3a	48.23±8.71a	0.626±0.119ab	0.770±0.114b	712.3±39.5c
T₃	258.8±25.1a	49.95±8.39a	0.610±0.044b	0.771±0.183b	683.3±41.5c
T₄	277.8±62.7a	62.08±9.09a	0.722±0.070a	1.110±0.078a	911.0±115.5ab
T₅	205.7±57.1a	46.74±17.98a	0.707±0.110ab	0.856±0.410ab	1042.3±39.5a

处理	总根长度/cm	总表面积/cm²	平均直径/mm	根系体积/cm³	根尖末端数/个
T₁	226.7±43.6a	47.17±14.09a	0.653±0.089ab	0.789±0.312b	793.0±73.0bc
T₂	233.3±27.3a	48.23±8.71a	0.626±0.119ab	0.770±0.114b	712.3±39.5c
T₃	258.8±25.1a	49.95±8.39a	0.610±0.044b	0.771±0.183b	683.3±41.5c
T₄	277.8±62.7a	62.08±9.09a	0.722±0.070a	1.110±0.078a	911.0±115.5ab
T₅	205.7±57.1a	46.74±17.98a	0.707±0.110ab	0.856±0.410ab	1042.3±39.5a

处理	L≤0.5 mm	0.5 mm<L≤1.0 mm	1.0 mm<L≤2.0 mm	L>2.0 mm
T₁	129.9±16.9a	23.4±8.3b	7.6±2.3ab	6.1±1.8a
T₂	143.1±46.5a	22.3±8.8b	5.7±1.9b	7.2±1.2a
T₃	155.5±93.3a	37.9±7.8a	8.4±3.1a	8.7±4.7a
T₄	171.3±20.5a	35.9±9.2a	10.3±3.9a	7.8±3.3a
T₅	189.6±14.7a	32.2±3.4a	9.8±1.3a	7.2±1.1a