Quality Grading Standard of Malania oleifera Seedlings

doi:10.11923/j.issn.2095-4050.cjas2023-0038

Abstract

Abstract:

Quality grading standards for Malania oleifera seedlings in line with the actual production needs is formulated, which can provide reference for M. oleifera seedling breeding and standardized production. 20 batches of M. oleifera seedlings were randomly selected from the main production area of M. oleifera in Funing County, Yunnan Province. 11 indicators were measured, including fresh weight, plant height, leaf number, leaf length, leaf width, ground diameter, main root length, main root diameter, lateral root number, chlorophyll content, and nitrogen content. The data were analyzed by using principal component and K-value clustering analysis, and then combined with actual production to determine the grading standards of M. oleifera seedlings. M. oleifera seedlings were divided into three levels with determining plant height, main root length, and ground diameter as the grading standards. Grade I seedlings: plant height≥ 33.33 cm, main root length≥ 8.00 cm, ground diameter≥ 0.23 mm; Grade II seedlings: 33.33 cm> plant height≥ 27.77 cm, 8.00 cm> main root length ≥ 7.90 cm, 0.23 mm> ground diameter≥ 0.21 mm; Grade III seedlings: 27.77 cm> plant height≥ 21.29 cm, 7.90 cm> main root length≥ 6.41 cm, 0.21 mm> ground diameter≥ 0.19 mm. In this study, the formulation of quality standards for seedlings is coincident with actual production and can provide a theoretical basis for the production of M. oleifera seedlings.

Key words: Malania oleifera, seedlings, quality grading standard, principal component analysis, cluster analysis

LI Ling, YANG Qingsong, CHEN Ding, PENG Cuixian, SUN Hongwei, ZHAO Dawei, TAO Yonghong. Quality Grading Standard of Malania oleifera Seedlings[J]. Journal of Agriculture, 2024, 14(1): 59-64.

Figures/Tables 8

References 19

[1]	唐婷范. 蒜头果有效成分及其生物活性研究[D]. 南宁: 广西大学, 2013.
[2]	王性炎, 樊金栓, 王姝清. 中国含神经酸植物开发利用研究[J]. 中国油脂, 2006, 31(3):3.
[3]	XU C Q, LIU H, ZHOU S S, et al. Genome sequence of Malania oleifera, a tree with great value for nervonic acid production[J]. Gigascience, 2019, 8(2):giy164.
[4]	LI Q, CHEN J, YU X ZH, et al. A mini review of nervonic acid: Source, production, and biological functions[J]. Food chemistry, 2019, 301(7):125286. doi: 10.1016/j.foodchem.2019.125286 URL
[5]	LEWKOWICZ N, PIATEK P, NAMIECINSKA M, et al. Naturally occurring nervonic acid ester improves myelin synthesis by human oligodendrocytes[J]. Cells, 2019, 786(8):2-17.
[6]	王性炎, 谢胜菊, 王高红. 中国富含神经酸的元宝枫籽油应用研究现状及前景[J]. 中国油脂, 2018, 43(12):4.
[7]	KASAI N, MIZUSHINA Y, SUGAWARA F, et al. Three-dimensional structural model analysis of the binding site of an inhibitor, nervonic acid, of both DNA polymerase beta and HIV-1 reverse transcriptase[J]. Journal of biochemistry, 2002, 132(5):819-828. doi: 10.1093/oxfordjournals.jbchem.a003292 pmid: 12417034
[8]	TANAKA K, SHIMIZU T, OHTSUKA Y, et al. Early dietary treatments with Lorenzo's oil and docosahexaenoic acid for neurological development in a case with Zellweger syndrome[J]. Brain & development, 2007, 29(9):586-589.
[9]	王建民, 胡晓凯, 王建林, 等. 生物活性物质神经酸钙的分离提取纯化生产工艺及其在治疗老年痴呆症中的应用[P]. 中国专利, CN101991564A,2011-03-30.
[10]	侯镜德, 陈至善. 神经酸与脑健康[M]. 北京: 中国科学技术出版社, 2006:3-10.
[11]	YAMAZAKI Y, KONDO K, MAEBA R, et al. The proportion of nervonic acid in serum lipids is associated with serum plasmalogen levels and metabolic syndrome[J]. Journal of oleo science, 2014, 63(5):527. pmid: 24770479
[12]	蒋水元, 李虹, 黄夕洋, 等. 两面针苗木分级标准的研究[J]. 福建林业科技, 2010, 37(4):4.
[13]	付玉嫔, 祁荣频, 李玉媛. 榉树容器苗苗木分级与种源研究[J]. 广西林业科学, 2005, 34(3):5.
[14]	江军, 邱苗苗, 李石华, 等. 赣南八角苗木分级标准研究[J]. 福建林业科技, 2016(2):177-180.
[15]	刘键锺, 曾建荣, 卢昌华, 等. 白木香苗木质量分级标准研究[J]. 种子, 2021, 40(5):110.
[16]	李勇鹏, 景跃波, 卯吉华, 等. 蒜头果半寄生特性研究[J]. 西南林业科学, 2019, 48(4):1-6.
[17]	INOUE Y, GUÉRIF M, BARET F, et al. Simple and robust methods for remote sensing of canopy chlorophyll content: A comparative analysis of hyperspectral data for different types of vegetation[J]. Plant cell environ, 2016, 39(12):2609-2623. doi: 10.1111/pce.v39.12 URL
[18]	THOMIDIS T, ZIOZIOU E, KOUNDOURAS S, et al. Effect of prohexadione-Ca on leaf chlorophyll content, gas exchange, berry size and composition, wine quality and disease susceptibility in Vitis vinifera L. cv Xinomavro[J]. Scientia horticulturae, 2018, 238:369-374. doi: 10.1016/j.scienta.2018.05.008 URL
[19]	赵乌英嘎, 红梅, 德海山, 等. 长期不同施氮水平对草原植物群落结构的影响[J]. 西北植物学报, 2020, 40(1):141-149.

批次	鲜重/g	株高/cm	叶片数/个	叶长/cm	叶宽/cm	地径/mm
1	0.718±0.198	29.950±5.002	12±2.330	11.413±0.933	4.438±0.540	0.205±0.026
2	0.794±0.182	31.263±3.373	12±1.847	10.450±0.840	4.625±0.477	0.224±0.059
3	0.890±0.174	33.325±4.281	12±1.808	11.363±0.980	4.775±0.373	0.242±0.040
4	0.766±0.135	31.713±3.074	10±2.376	11.138±0.534	4.688±0.248	0.228±0.032
5	0.726±0.110	28.450±5.334	10±3.162	10.713±1.352	4.663±0.585	0.234±0.040
6	0.725±0.110	30.975±5.143	10±2.435	11.438±1.330	4.638±0.585	0.228±0.036
7	0.754±0.204	31.044±5.044	10±2.333	10.089±1.622	4.456±0.422	0.210±0.029
8	0.678±0.168	26.500±4.900	9±1.394	9.811±0.874	4.489±0.352	0.195±0.034
9	0.537±0.192	21.711±7.298	9±1.054	10.133±1.291	4.044±0.737	0.201±0.025
10	0.766±0.222	28.578±5.757	10±2.062	10.778±0.938	4.467±0.534	0.214±0.032
11	0.751±0.167	29.488±4.989	8±1.923	10.988±1.331	4.613±0.356	0.218±0.044
12	0.688±0.206	29.922±5.284	11±1.753	9.900±1.296	4.478±0.466	0.211±0.043
13	0.632±0.138	26.067±3.352	9±1.616	9.867±1.395	4.456±0.500	0.198±0.012
批次	鲜重/g	株高/cm	叶片数/个	叶长/cm	叶宽/cm	地径/mm
14	0.720±0.149	28.688±3.993	9±2.997	10.863±1.855	4.875±0.396	0.201±0.017
15	0.626±0.105	28.113±2.645	9±2.417	10.000±1.038	4.413±0.402	0.197±0.022
16	0.777±0.144	30.078±2.151	8±2.333	10.378±1.859	4.667±0.477	0.224±0.031
17	0.760±0.238	29.688±4.464	9±2.138	9.625±2.275^Be	4.688±0.629	0.232±0.054
18	0.719±0.199	29.100±3.195	8±2.028	10.200±1.598	4.467±0.682	0.209±0.033
19	0.739±0.129	28.467±3.878	10±2.603	9.800±1.635	4.367±0.495	0.207±0.036
20	0.698±0.128	28.488±3.681	9±1.069	9.638±0.941	4.525±0.545	0.222±0.040
批次	主根长/cm	主根根粗/mm	侧根数/个	叶绿素含量/SPAD	氮含量/(mg/g)
1	8.150±1.416	0.390±0.071	10±3.207	45.936±4.327	14.223±1.389
2	8.575±2.148	0.411±0.082	8±1.512	47.111±3.480	14.659±1.055
3	10.388±3.047	0.376±0.055	7±1.553	44.580±4.712	13.900±1.433
4	8.975±2.095	0.450±0.043	8±1.408	45.775±4.175	14.279±1.252
5	7.363±3.087	0.460±0.039	7±2.26	49.059±6.084	15.276±1.842
6	7.938±1.626	0.408±0.059	6±2.121	50.029±6.043	15.549±1.805
7	8.756±2.158	0.389±0.111	9±2.386	49.772±4.123	15.441±1.265
8	7.000±2.254	0.420±0.078	7±1.900	47.597±5.478	14.822±1.638
9	5.956±2.280	0.332±0.036	6±2.236	52.071±4.809	16.159±1.441
10	6.733±1.899	0.422±0.065	7±1.965	50.726±1.858	15.797±0.518
11	8.313±2.198	0.384±0.057	8±2.264	50.953±4.241	15.845±1.244
12	9.156±3.405	0.353±0.053	8±2.421	48.014±5.902	15.293±1.566
13	7.144±2.925	0.380±0.105	7±1.900	45.890±3.092	14.381±1.012
14	8.263±2.667	0.371±0.057	8±3.292	50.748±5.647	15.773±1.693
15	7.925±2.330	0.354±0.056	8±2.726	48.096±4.491	14.976±1.344
16	8.300±2.119	0.432±0.065	10±2.000	48.799±3.321	15.163±0.976
17	5.363±1.705	0.468±0.088	7±2.696	50.755±5.304	15.776±1.589
18	5.522±2.446	0.444±0.039	8±3.154	53.886±5.255	16.601±1.547
19	6.978±2.631	0.398±0.087	8±2.906	45.996±4.312	14.343±1.298
20	7.188±1.539	0.422±0.060	6±2.264	46.444±12.079	15.654±1.017

批次	鲜重/g	株高/cm	叶片数/个	叶长/cm	叶宽/cm	地径/mm
1	0.718±0.198	29.950±5.002	12±2.330	11.413±0.933	4.438±0.540	0.205±0.026
2	0.794±0.182	31.263±3.373	12±1.847	10.450±0.840	4.625±0.477	0.224±0.059
3	0.890±0.174	33.325±4.281	12±1.808	11.363±0.980	4.775±0.373	0.242±0.040
4	0.766±0.135	31.713±3.074	10±2.376	11.138±0.534	4.688±0.248	0.228±0.032
5	0.726±0.110	28.450±5.334	10±3.162	10.713±1.352	4.663±0.585	0.234±0.040
6	0.725±0.110	30.975±5.143	10±2.435	11.438±1.330	4.638±0.585	0.228±0.036
7	0.754±0.204	31.044±5.044	10±2.333	10.089±1.622	4.456±0.422	0.210±0.029
8	0.678±0.168	26.500±4.900	9±1.394	9.811±0.874	4.489±0.352	0.195±0.034
9	0.537±0.192	21.711±7.298	9±1.054	10.133±1.291	4.044±0.737	0.201±0.025
10	0.766±0.222	28.578±5.757	10±2.062	10.778±0.938	4.467±0.534	0.214±0.032
11	0.751±0.167	29.488±4.989	8±1.923	10.988±1.331	4.613±0.356	0.218±0.044
12	0.688±0.206	29.922±5.284	11±1.753	9.900±1.296	4.478±0.466	0.211±0.043
13	0.632±0.138	26.067±3.352	9±1.616	9.867±1.395	4.456±0.500	0.198±0.012
批次	鲜重/g	株高/cm	叶片数/个	叶长/cm	叶宽/cm	地径/mm
14	0.720±0.149	28.688±3.993	9±2.997	10.863±1.855	4.875±0.396	0.201±0.017
15	0.626±0.105	28.113±2.645	9±2.417	10.000±1.038	4.413±0.402	0.197±0.022
16	0.777±0.144	30.078±2.151	8±2.333	10.378±1.859	4.667±0.477	0.224±0.031
17	0.760±0.238	29.688±4.464	9±2.138	9.625±2.275^Be	4.688±0.629	0.232±0.054
18	0.719±0.199	29.100±3.195	8±2.028	10.200±1.598	4.467±0.682	0.209±0.033
19	0.739±0.129	28.467±3.878	10±2.603	9.800±1.635	4.367±0.495	0.207±0.036
20	0.698±0.128	28.488±3.681	9±1.069	9.638±0.941	4.525±0.545	0.222±0.040
批次	主根长/cm	主根根粗/mm	侧根数/个	叶绿素含量/SPAD	氮含量/(mg/g)
1	8.150±1.416	0.390±0.071	10±3.207	45.936±4.327	14.223±1.389
2	8.575±2.148	0.411±0.082	8±1.512	47.111±3.480	14.659±1.055
3	10.388±3.047	0.376±0.055	7±1.553	44.580±4.712	13.900±1.433
4	8.975±2.095	0.450±0.043	8±1.408	45.775±4.175	14.279±1.252
5	7.363±3.087	0.460±0.039	7±2.26	49.059±6.084	15.276±1.842
6	7.938±1.626	0.408±0.059	6±2.121	50.029±6.043	15.549±1.805
7	8.756±2.158	0.389±0.111	9±2.386	49.772±4.123	15.441±1.265
8	7.000±2.254	0.420±0.078	7±1.900	47.597±5.478	14.822±1.638
9	5.956±2.280	0.332±0.036	6±2.236	52.071±4.809	16.159±1.441
10	6.733±1.899	0.422±0.065	7±1.965	50.726±1.858	15.797±0.518
11	8.313±2.198	0.384±0.057	8±2.264	50.953±4.241	15.845±1.244
12	9.156±3.405	0.353±0.053	8±2.421	48.014±5.902	15.293±1.566
13	7.144±2.925	0.380±0.105	7±1.900	45.890±3.092	14.381±1.012
14	8.263±2.667	0.371±0.057	8±3.292	50.748±5.647	15.773±1.693
15	7.925±2.330	0.354±0.056	8±2.726	48.096±4.491	14.976±1.344
16	8.300±2.119	0.432±0.065	10±2.000	48.799±3.321	15.163±0.976
17	5.363±1.705	0.468±0.088	7±2.696	50.755±5.304	15.776±1.589
18	5.522±2.446	0.444±0.039	8±3.154	53.886±5.255	16.601±1.547
19	6.978±2.631	0.398±0.087	8±2.906	45.996±4.312	14.343±1.298
20	7.188±1.539	0.422±0.060	6±2.264	46.444±12.079	15.654±1.017

项目	鲜重/g	株高/cm	叶片数/片	叶长/cm	叶宽/cm	地径/mm	主根长/cm	主根根粗/mm	侧根数/个	叶绿素含量/SPAD	氮含量/(mg/g)
极小值	0.34	13.40	4.00	5.20	3.20	0.11	2.30	0.15	3.00	36.20	11.40
极大值	1.15	42.00	16.00	13.60	5.80	0.32	17.10	0.59	14.00	61.80	19.10
均值	0.72	29.02	9.64	10.41	4.54	0.21	7.68	0.40	7.64	48.83	15.20
标准差	0.1752	4.8907	2.2782	1.4147	0.5069	0.0361	2.5457	0.0742	2.4821	4.9694	1.4808
变异系数/%	24.27	16.85	23.63	13.59	11.18	16.84	33.16	18.42	32.49	10.18	9.74

项目	鲜重/g	株高/cm	叶片数/片	叶长/cm	叶宽/cm	地径/mm	主根长/cm	主根根粗/mm	侧根数/个	叶绿素含量/SPAD	氮含量/(mg/g)
极小值	0.34	13.40	4.00	5.20	3.20	0.11	2.30	0.15	3.00	36.20	11.40
极大值	1.15	42.00	16.00	13.60	5.80	0.32	17.10	0.59	14.00	61.80	19.10
均值	0.72	29.02	9.64	10.41	4.54	0.21	7.68	0.40	7.64	48.83	15.20
标准差	0.1752	4.8907	2.2782	1.4147	0.5069	0.0361	2.5457	0.0742	2.4821	4.9694	1.4808
变异系数/%	24.27	16.85	23.63	13.59	11.18	16.84	33.16	18.42	32.49	10.18	9.74

	鲜重	株高	叶片数	叶长	叶宽	地径	主根长	主根根粗	侧根数	叶绿素含量	氮含量
鲜重	1.000
株高	0.878	1.000
叶片数	0.419	0.487	1.000
叶长	0.448	0.473	0.410	1.000
叶宽	0.719	0.706	0.096	0.422	1.000
地径	0.724	0.652	0.297	0.400	0.584	1.000
主根长	0.498	0.645	0.603	0.512	0.447	0.302	1.000
主根根粗	0.443	0.333	-0.182	0.000	0.434	0.525	-0.314	1.000
侧根数	0.278	0.375	0.120	0.159	0.131	-0.145	0.346	0.008	1.000
叶绿素含量	-0.263	-0.319	-0.586	-0.077	-0.154	-0.150	-0.559	0.073	-0.115	1.000
氮含量	-0.320	-0.352	-0.634	-0.221	-0.168	-0.118	-0.575	0.078	-0.260	0.930	1.000