Abstract:

A long-term fertilizer experiment was performed to investigate the effect of organic fertilizer application (oilcake manure and rice straw) on soil bacterial community diversity and structure. The results showed that soil bacterial diversity increased by the combined application of organic (oilcake manure or rice straw) and chemical fertilizers compared to only chemical fertilizers application, with rice straw returning exhibiting the highest efficacy. Meanwhile, the combined application of organic and chemical fertilizers significantly altered soil bacterial community structures. Specifically, the treatment of combining rice straw returning and chemical fertilizers exhibited twice as much unique operational taxonomic units (OTUs) compared to other treatments. At the taxonomic level of bacterial community family, there was an increase in relative abundance of Hydrogenophilaceae and Intrasporangiaceae in response to the combined use of rice straw and chemical fertilizers. In conclusion, employing a combination of organic and chemical fertilizers can effectively maintain soil bacterial diversity while modifying bacterial community structure. The combined application of rice straw returning and chemical fertilizers is a feasible management strategy for preserving soil bacterial diversity in tobacco fields, thereby improving both soil structure and fertilizer conservation capacity.

Key words: tobacco field, organic materials, soil, bacterial community, microbial structure, fertilizer cake, straw, soil bacterial diversity, tobacco field conservation, impact effects