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农学学报

农学学报 ›› 2015, Vol. 5 ›› Issue (9): 110-116.doi: 10.11923/j.issn.2095-4050.cjas14110017

所属专题： 生物技术

• 畜牧 兽医 水产 • 上一篇    下一篇

升温对不同管足颜色中间球海胆家系和红、白管足中间球海胆免疫相关酶活性及MDA含量影响的初步研究

丁文君,丁君,张伟杰,丁玉龙,何鹏   

  1. 大连海洋大学,大连海洋大学,大连海洋大学,大连海洋大学,大连海洋大学
  • 收稿日期:2014-11-26 修回日期:2015-03-07 接受日期:2015-03-18 出版日期:2015-09-28 发布日期:2015-09-28
  • 通讯作者: 丁君 E-mail:mengnvsuibian@163.com
  • 基金资助:
    国家“863”计划重大项目(2012AA10A412)；辽宁省农业攻关及成果产业化项目(201401549)

A preliminary study of rising temperature on immune-related enzyme and MDA content in family of sea urchins(Strongylocentrotus intermedius) with different color tube feet and red, white Strongylocentrotus intermedius

  • Received:2014-11-26 Revised:2015-03-07 Accepted:2015-03-18 Online:2015-09-28 Published:2015-09-28

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摘要： 为研究升温对不同管足颜色中间球海胆家系和红、白管足中间球海胆免疫相关酶活性及MDA含量的影响,实验设置不同温度梯度(15、20、22、24及26℃),分别对不同管足颜色海胆家系和红、白管足海胆体腔液中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、溶菌酶(LZM)及丙二醛(MDA)含量进行测定。结果显示：(1)随着水温升高,RR、RW、WR和WW家系中间球海胆SOD、CAT、POD、LZM活性均呈先升高后降低的趋势。SOD活性范围为2.39 ~4.36U/ml、2.79 ~5.17U/ml、2.71 ~4.97U/ml、3.66 ~5.69U/ml；CAT活性范围为63.02 ~112.76U/ml、62.47 ~169.37U/ml、66.40 ~140.77U/ml、72.29 ~149.20U/ml；POD活性范围为1.65 ~14.59U/ml、0.89 ~13.88U/ml、2.08 ~14.68U/ml、2.49 ~15.62U/ml；LZM活性范围为9.82 ~109.23U/ml、12.10 ~110.37U/ml、7.82 ~112.16U/ml、11.18 ~107.83U/ml；RR、RW、WR家系中间球海胆MDA含量呈先降低后升高的趋势,含量范围为0.97 ~2.34nmol/ml、0.82 ~2.40nmol/ml、0.96 ~2.23nmol/ml,WW家系中间球海胆MDA含量呈缓慢升高的趋势,含量范围为1.55 ~2.60nmol/ml。(2) 红、白管足中间球海胆SOD 、CAT、POD、LZM活性也均呈先升高后降低的趋势,红管足海胆MDA含量呈先降低后升高的趋势,白管足海胆MDA含量则呈升高的趋势。(3) 随着水温升高,RR家系海胆SOD活性对升温胁迫响应早于WW家系海胆；RW家系海胆CAT活性高于WW家系海胆；RR家系海胆MDA含量低于WW家系海胆MDA含量；红管足海胆SOD、POD活性均高于白管足海胆,其中POD活性变化差异显著(P<0.05)；26℃时,红管足海胆MDA含量显著低于白管足海胆MDA含量 (P<0.05)。以上结果表明,以红管足海胆为亲本培育的海胆家系对升温响应更灵敏、更耐高温。

关键词: 水溶肥, 水溶肥, 草莓, 产量, 可溶性固形物, 病害

Abstract: The experiment studied the change of immune-related enzyme and MDA content in rising temperature. The activities of SOD, CAT, POD, LZM and the content of MDA in family of sea urchins (Strongylocentrotus intermedius) with different color tube feet were analyzed. The result show that:(1)In rising temperature, the change of SOD,CAT,POD,LZM were trend to increased and to decreased in RR, RW, WR, WW family of sea urchins, the activities of SOD were 2.39 ~4.36U/ml, 2.79 ~5.17U/ml, 2.71 ~4.97U/ml,3.66 ~5.69U/ml respectively; the activities of CAT were 63.02 ~112.76U/ml, 62.47 ~169.37U/ml, 66.40 ~140.77U/ml, 72.29 ~149.20U/ml respectively; the activities of POD were 1.65 ~14.59U/ml, 0.89 ~13.88U/ml, 2.08 ~14.68U/ml, 2.49 ~15.62U/ml respectively; the activities of LZM were 9.82 ~109.23U/ml, 12.10 ~110.37U/ml, 7.82 ~112.16U/ml, 11.18 ~107.83U/ml respectively; the activity of MDA first reduced and then increased in RR, RW and WR family, the content of MDA were 0.97 ~2.34nmol/ml, 0.82 ~2.40nmol/ml, 0.96 ~2.23nmol/ml, the activity of MDA in WW family was trend to increased, the content of MDA was 1.55 ~2.60nmol/ml. (2)The change of SOD,CAT,POD,LZM were trend to increased and to decreased in red and white tube feet S. intermedius, the activity of MDA first reduced and then increased in red tube feet sea urchins, and increased in white tube feet sea urchins. (3) In rising temperature, the activity of SOD, CAT in RR family was more sensitive than WW family, the content of MDA in RR family was lower than in WW family; the activity of SOD, POD in red tube feet sea urchins was more sensitive than in white tube feet sea urchins(P<0.05); in 26℃ the activity of POD had significant difference(P<0.05). These result revealed that family of sea urchins presented in the aspect temperature sensitively and adaptability strongly whose foster parents were the red tube feet sea urchins.