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农学学报 ›› 2020, Vol. 10 ›› Issue (6): 15-21.doi: 10.11923/j.issn.2095-4050.cjas20191200302

所属专题: 生物技术 马铃薯

• 农艺科学/生理生化 • 上一篇    下一篇

不同光周期处理对马铃薯组培苗生长和生理生化特性的影响

马永珍1, 王芳1,2,3,4,5(), 王舰1,2,3,4,5   

  1. 1 青海大学,西宁 810016
    2 青海省农林科学院,西宁 810016
    3 青海大学省部共建三江源生态与高原农牧业国家重点实验室,西宁 810016
    4 青藏高原生物技术教育部重点实验室,西宁 810016
    5 青海省马铃薯育种重点实验室,西宁 810016
  • 收稿日期:2019-12-19 出版日期:2020-06-20 发布日期:2020-07-09
  • 通讯作者:
    王芳,女,1971年出生,陕西西安人,副研究员,硕士,主要从事马铃薯遗传育种、马铃薯种质资源创新、马铃薯脱毒种薯生产及相关研究等工作。通信地址:810016 青海省西宁市城北区宁大路251号青海大学,Tel:13997048865,E-mail:
  • 作者简介:

    马永珍,男,1994年出生,甘肃天水人,在读硕士研究生,研究方向:马铃薯遗传育种。通信地址:810016 青海省西宁市城北区宁大路251号青海大学,Tel:18997075771,E-mail:1773655102@qq.com

  • 基金资助:
    国家自然科学基金(31660417); 现代农业产业技术体系(CARS-9); 专用型马铃薯产业高质量发展关键技术研发与示范(2019-NK-A1)

Light Cycle Treatments: Effects on the Growth and Physiological and Biochemical Characteristics of Potato Tissue Culture Seedling

Ma Yongzhen1, Wang Fang1,2,3,4,5(), Wang Jian1,2,3,4,5   

  1. 1 Qinghai University, Xining 810016, Qinghai, China
    2 Qinghai Academy of Agriculture and Forestry Sciences, Xining 810016, Qinghai, China
    3 State Key Laboratory of Agriculture and Animal Husbandry in San Jiang Yuan Ecology and Plateau, Xining 810016, Qinghai, China
    4 Key Laboratory of Biotechnology Ministry of Education of Qinghai-Tibet Plateau, Xining 810016, Qinghai, China
    5 Key Laboratory of Potato Breeding, Xining 810016, Qinghai, China
  • Received:2019-12-19 Online:2020-06-20 Published:2020-07-09

摘要:

为了研究不同品种马铃薯组培苗生长和生理生化特性对光周期处理的响应,以‘国外2号’、‘闽薯1号’以及‘青薯9号’马铃薯组培苗为试验材料,设置8 h/d、12 h/d和16 h/d三种光周期处理,培养35天时测定并分析生长和生理指标数据。结果表明:(1)通过对叶片数、鲜重、茎粗、茎节数、根数、干重、根长、株高8项生长指标综合分析得出,‘国外2号’、‘闽薯1号’、和‘青薯9号’都随着光周期的延长综合生长性能有所增加,在16 h/d光照处理下,3个品种的综合生长性能均达到最优。(2)16 h/d光照处理下可以显著促进‘国外2号’马铃薯可溶性蛋白的合成,在8 h/d光照处理下可以显著促进‘闽薯1号’和‘青薯9号’马铃薯可溶性蛋白的合成。由于‘国外2号’和‘青薯9号’马铃薯两种氧化酶类对光周期不太敏感,SOD与CAT活性没有呈现一定的规律性。‘闽薯1号’马铃薯在12 h/d光照处理下SOD活性最高,MDA含量积累最少。

关键词: 光周期, 马铃薯, 组培苗, 生长指标, 生理生化指标

Abstract:

To investigate the response of the growth and physiological and biochemical characteristics of potato tissue culture seedlings of different varieties to photoperiod treatments, ‘Guowai No.2’, ‘Minshu No.1’ and ‘Qingshu No.9’ potato tissue culture seedlings were used as the experimental materials. Three photoperiod treatments of 8 h/d, 12 h/d and 16 h/d were set up, and the growth and physiological index data were measured and analyzed for 35 days of culture. The results showed that: (1) based on the comprehensive analysis of eight growth indicators of leaf number, fresh weight, stem thickness, stem node number, root number, dry weight, root length and plant height, the comprehensive growth performance of ‘Guowai No.2’, ‘Minshu No.1’ and ‘Qingshu No.9’ all increased with the extension of the photoperiod, under the 16 h/d light treatment, the comprehensive growth performance of the three varieties reached the optimal; (2) 16 h/d light treatment could significantly promote the soluble protein synthesis of ‘Guowai No.2’, and 8 h/d light treatment could significantly promote the soluble protein synthesis of ‘Minshu No.1’ and ‘Qingshu No.9’, the activities of SOD and CAT did not show certain regularity due to the fact that the two kinds of oxidase in ‘Guowai No. 2’ and ‘Qingshu No.9’ were not sensitive to photoperiod. For ‘Minshu No.1’, the activity of SOD was the highest and the accumulation of MDA was the least under 12 h/d light.

Key words: Photoperiod, Potato, Tissue Culture Seedling, Growth Index, Physiological and Biochemical Index

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