超表达StCYS1对马铃薯生长发育及酶促褐变的影响

doi:10.11923/j.issn.2095-4050.cjas20200300067

农学学报 ›› 2020, Vol. 10 ›› Issue (6): 86-93.doi: 10.11923/j.issn.2095-4050.cjas20200300067

所属专题：生物技术；马铃薯

• 农业工程/农业机械/生物技术/食品科学 • 上一篇下一篇

超表达StCYS1对马铃薯生长发育及酶促褐变的影响

刘敏敏¹, 李广存², 刘士扬¹, 董田田¹, 王庆国¹(), 刘佩¹

¹ 山东农业大学食品科学与工程学院/山东省高校食品加工技术与质量控制重点实验室,山东泰安 271018
² 中国农业科学院蔬菜花卉研究所,北京 100081

收稿日期:2020-03-18 出版日期:2020-06-20 发布日期:2020-07-09
通讯作者:
王庆国,男,1965年出生,山东青州人,教授,硕士,主要从事果品蔬菜贮藏及加工理论与技术研究。通信地址：271018 山东省泰安市泰山区岱宗大街61号山东农业大学食品科学与工程学院,E-mail： wqgyyy@126.com。
作者简介:
刘敏敏,女,1994年出生,山东潍坊人,硕士,主要从事果蔬采后贮藏与加工研究。通信地址：271018 山东省泰安市泰山区岱宗大街61号山东农业大学食品科学与工程学院,E-mail：530465553@qq.com
基金资助:
山东省现代农业产业技术体系“薯类产业创新团队建设”(SDAIT-16-11); 山东省自然科学基金面上项目“鲜切马铃薯褐变相关基因的筛选及功能分析”(ZR2013CM02); 山东省泰山产业领军人才工程“系列安全果蔬保鲜剂创制及应用技术研究”(LYNJ201702); 国家自然科学基金青年基金项目“马铃薯半胱氨酸蛋白酶抑制剂CPI的转录调控机理研究”(31901752)

Effects of Overexpression of StCYS1 on Potato Growth and Development and Enzymatic Browning

Liu Minmin¹, Li Guangcun², Liu Shiyang¹, Dong Tiantian¹, Wang Qingguo¹(), Liu Pei¹

¹ College of Food Science and Engineering, Shandong Agricultural University/ Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, Tai’an 271018, Shandong, China
² Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2020-03-18 Online:2020-06-20 Published:2020-07-09

摘要/Abstract

摘要：

为了探究半胱氨酸蛋白酶抑制子基因StCYS1对马铃薯植株生长发育及块茎酶促褐变的影响,本研究以超表达StCYS1和野生型二倍体马铃薯‘MDS’植株为材料,对植株光合指标、根系发育、产量及块茎褐变情况、PPO活性、总抗氧化能力和游离氨基酸含量进行测定。本研究发现,与野生型马铃薯植株相比,超表达株系的光合能力显著提高,根系更加发达,产量更高。与野生型相比,超表达株系块茎的酶促褐变减轻,PPO活性升高,游离脯氨酸含量和总抗氧化能力显著提高,游离酪氨酸含量下降。试验结果表明,超表达StCYS1株系显著提高了马铃薯植株的光合作用,促进了根系和植株发育,进而提高了马铃薯块茎的产量,超表达StCYS1有效提高了马铃薯块茎的抗氧化能力,降低了游离酪氨酸含量,显著抑制了马铃薯的酶促褐变。

关键词: 马铃薯, StCYS1, 超表达, 生长发育, 酶促褐变

Abstract:

To explore the effect of cysteine protease inhibitor gene StCYS1 on the growth and development of potato plants and the enzymatic browning of tubers, StCYS1 overexpressed and wild type ‘MDS’ diploid potato plants were used as materials to study the photosynthetic index, root development, yield, browning of tubers after harvest, PPO activity, total antioxidant capacity and free amino acid content. Compared with wild-type potato plants, the photosynthetic capacity of overexpression lines was significantly improved, the root system was more developed and the yield was higher. Compared with the wild type, the overexpression lines showed less enzymatic browning, higher PPO activity, higher free proline content and total antioxidant capacity, and lower free tyrosine content. The results show that the overexpression of StCYS1 could significantly increase the photosynthesis of potato plants, promote the development of roots and plants, and then increase the yield of potato tubers. The overexpression of StCYS1 effectively improves the antioxidant capacity of potato tubers, reduces the content of free tyrosine, and significantly inhibits the enzymatic browning of potatoes.

Key words: Potato, StCYS1, Overexpression, Growth and Development, Enzymatic Browning

中图分类号:

S532

刘敏敏, 李广存, 刘士扬, 董田田, 王庆国, 刘佩. 超表达StCYS1对马铃薯生长发育及酶促褐变的影响[J]. 农学学报, 2020, 10(6): 86-93.

Liu Minmin, Li Guangcun, Liu Shiyang, Dong Tiantian, Wang Qingguo, Liu Pei. Effects of Overexpression of StCYS1 on Potato Growth and Development and Enzymatic Browning[J]. Journal of Agriculture, 2020, 10(6): 86-93.

图/表 9

参考文献 32

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株系	总根长/cm	总表面积/cm²	平均直径/mm	总体积/cm³
WT	504.40±14.73d	156.43±4.32d	0.65±0.05c	2.18±0.97c
OE1	1003.43±12.06b	213.71±3.48b	0.76±0.06b	3.67±0.33b
OE2	1221.05±19.31a	292.25±8.33a	0.87±0.03a	6.87±0.91a
OE3	831.87±18.56c	199.30±7.29c	0.64±0.02c	3.73±0.28b

株系	总根长/cm	总表面积/cm²	平均直径/mm	总体积/cm³
WT	504.40±14.73d	156.43±4.32d	0.65±0.05c	2.18±0.97c
OE1	1003.43±12.06b	213.71±3.48b	0.76±0.06b	3.67±0.33b
OE2	1221.05±19.31a	292.25±8.33a	0.87±0.03a	6.87±0.91a
OE3	831.87±18.56c	199.30±7.29c	0.64±0.02c	3.73±0.28b

株系	单株薯重/g	单株结薯数量/个	最大单薯质量/g
WT	9.67±1.41d	8.80±0.74d	4.60
OE1	14.25±0.91b	23.33±1.55a	6.81
OE2	19.63±1.63a	17.86±1.47b	11.48
OE3	10.93±0.74c	10.33±0.34c	7.04

株系	单株薯重/g	单株结薯数量/个	最大单薯质量/g
WT	9.67±1.41d	8.80±0.74d	4.60
OE1	14.25±0.91b	23.33±1.55a	6.81
OE2	19.63±1.63a	17.86±1.47b	11.48
OE3	10.93±0.74c	10.33±0.34c	7.04

差异氨基酸种类	WT	OE1	OE2	OE3
天冬氨酸(Asp)	0.37±0.014b	0.41±0.020a	0.42±0.018a	0.42±0.008a
苏氨酸(Thr)	0.04±0.006c	0.13±0.003a	0.15±0.003a	0.10±0.009b
脯氨酸(Pro)	0.19±0.001c	0.25±0.002b	0.28±0.015a	0.28±0.011a
亮氨酸(Leu)	0.037±0.001c	0.043±0.001a	0.040±0.002b	0.041±0.002b
甘氨酸(Gly)	0.11±0.003a	0.04±0.001c	0.04±0.001c	0.06±0.002b
组氨酸(His)	0.055±0.002a	0.048±0.003b	0.051±0.001b	0.050±0.000b
酪氨酸(Tyr)	0.11±0.005a	0.03±0.002c	0.03±0.001bc	0.04±0.001b
缬氨酸(Val)	0.040±0.000a	0.010±0.001c	0.012±0.000b	0.012±0.001b
蛋氨酸(Met)	0.10±0.007a	0.03±0.002c	0.04±0.001b	0.03±0.003c
异亮氨酸(Ile)	0.06±0.005a	0.02±0.000c	0.03±0.001b	0.03±0.000b
苯丙氨酸(Phe)	0.08±0.011a	0.04±0.004c	0.05±0.003b	0.04±0.002c

超表达StCYS1对马铃薯生长发育及酶促褐变的影响

Effects of Overexpression of StCYS1 on Potato Growth and Development and Enzymatic Browning

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