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农学学报

农学学报 ›› 2012, Vol. 2 ›› Issue (5): 24-28.

所属专题： 生物技术； 玉米

• 农艺科学 作物遗传育种 生理生化 • 上一篇    下一篇

抑制玉米幼胚愈伤组织褐化的研究

宋钦虎 郭新梅 裴玉贺 宋希云   

  • 收稿日期:2011-11-07 修回日期:2012-02-24 出版日期:2012-05-20 发布日期:2012-05-20
  • 基金资助:

    青岛市支撑，山东省良种产业化工程，山东省泰山学者项目

Primary Study on Reducing Browning of Callus Induced from Maize Immature Embryo

  • Received:2011-11-07 Revised:2012-02-24 Online:2012-05-20 Published:2012-05-20

PDF (PC)

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摘要：

褐化是植物组织培养中一种常见的问题，直接影响组织培养能否成功。为了控制植物组织培养中的褐化现象，选取‘丹黄25’、P138、H21 3个玉米自交系幼胚为外植体，着重探讨胚龄，2,4-D、甘露醇、L-脯氨酸和AgNO3浓度对玉米幼胚愈伤组织褐化的影响。结果表明，选取授粉11天的‘丹黄25’幼胚、13天的P138和H21幼胚接种到诱导培养基中，待长出愈伤后转移到添加2 mg/L 2,4-D、10 g/L甘露醇、10 mg/L L-脯氨酸、10 mg/L硝酸银的继代培养基上，愈伤组织褐化率显著降低，表明培养基的种类及添加物对愈伤组织的生长有重要影响。

关键词: 甜高粱, 甜高粱, 铅, 镉, 生长, 重金属含量

Abstract:

Material browning is a universal phenomenon in plant-tissue culture, and it plays a vital role in plant-tissue culture. To control the browing of plant-tissue culture, the immature embryos of three maize inbred lines (‘Danhuang25’, P138, H21) were used in the experiment. Embryo age, 2,4-D concentration, mannitol concentration, L-proline and silver nitrate concentration on the browning of maize calli were studied. The results showed that the embryos selected from ‘Danhuang 25’ (11-day after self-pollination), P138 and H21 (13-day after self-pollination) were inoculated into induction medium and then their callus browning could be significantly inhibited by addition of 2 mg/L 2,4-D, 10 g/L mannitol, 10 mg/L L-proline, 10 mg/L silver nitrate in subculture medium, indicate that the type of medium and additives have a major impact on callus growth.