Abstract:

Fungal disease is one of the important obstacles for high and stable yield of rice. In order to study the occurrence of fungi in rice seeds, the phylogenetic analysis and functional evaluation of a fungus isolated from rice seeds were carried out. On the basis of morphological identification, the target fungal sequence was amplified by PCR with ITS1/ITS4, and the phylogenetic tree was constructed by neighbor-joining (NJ) and analyzing the sequence. The morphological characteristics of strain S-51 were very similar to those of Microdochium nivale in the literature, and the sequence alignment showed that the target fungi were similar to M. nivale, and the similarity rate was 100%. M. nivale and M. musae had the closest genetic relationship on the same branch as the strain of snow mold leaf blight. Sequence analysis and phylogenetic tree construction based on ITS fragments and combined with morphological characteristics could identify S-51 strain isolated from rice seeds as M. nivale.

Key words: Rice Seed, Microdochium nivale, ITS, Taxonomic Status, Species Identification